Author response: Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)

AbstractThe internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our system through functional imaging of neural activity during visually evoked and prey capture behaviors in larval zebrafish.

Download Full-text

Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)

eLife ◽

10.7554/elife.28158 ◽

2017 ◽

Vol 6 ◽

Cited By ~ 82

Author(s):

Lin Cong ◽

Zeguan Wang ◽

Yuming Chai ◽

Wei Hang ◽

Chunfeng Shang ◽

...

Keyword(s):

Functional Imaging ◽

Danio Rerio ◽

Neural Activity ◽

Prey Capture ◽

Brain Dynamics ◽

3D Tracking ◽

Whole Brain ◽

Larval Zebrafish ◽

Volume Imaging ◽

Freely Behaving

The internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here, we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our system through functional imaging of neural activity during visually evoked and prey capture behaviors in larval zebrafish.

Download Full-text

Noninvasive recording of the Mauthner neurone action potential in larval zebrafish

Journal of Experimental Biology ◽

10.1242/jeb.101.1.83 ◽

1982 ◽

Vol 101 (1) ◽

pp. 83-92 ◽

Cited By ~ 2

Author(s):

J. I. Prugh ◽

C. B. Kimmel ◽

W. K. Metcalfe

Keyword(s):

Neural Activity ◽

Action Potentials ◽

Cell Function ◽

Recording Site ◽

M Cell ◽

Larval Zebrafish ◽

System 2 ◽

The Central Nervous System ◽

Freely Behaving ◽

M Spike

We describe the identification of Mauthner (M-) cell action potentials in an intact zebrafish larva, utilizing recording electrodes located outside the fish: 1. The externally recorded spike occurs at approximately the same time, and its waveform changes with recording site in the same way, as the extracellular M-spike recorded within the central nervous system. 2. The externally recorded M-spike may be readily distinguished from other forms of neural activity. 3. The M-spike can be identified in recordings from unrestrained larvae. This finding permits the direct study of M-cell function in the freely behaving animal.

Download Full-text