Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish

2016 ◽  
pp. 307-320 ◽  
Author(s):  
Michael B. Orger ◽  
Ruben Portugues
Keyword(s):  
Neural Activity ◽  
Whole Brain ◽  
Larval Zebrafish

scholarly journals Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)

2017 ◽  
Author(s):  
Lin Cong ◽  
Zeguan Wang ◽  
Yuming Chai ◽  
Wei Hang ◽  
Chunfeng Shang ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Danio Rerio ◽  
Neural Activity ◽  
Prey Capture ◽  
Brain Dynamics ◽  
3D Tracking ◽  
Whole Brain ◽  
Larval Zebrafish ◽  
Volume Imaging ◽  
Freely Behaving

AbstractThe internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our system through functional imaging of neural activity during visually evoked and prey capture behaviors in larval zebrafish.

scholarly journals Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Lin Cong ◽  
Zeguan Wang ◽  
Yuming Chai ◽  
Wei Hang ◽  
Chunfeng Shang ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Danio Rerio ◽  
Neural Activity ◽  
Prey Capture ◽  
Brain Dynamics ◽  
3D Tracking ◽  
Whole Brain ◽  
Larval Zebrafish ◽  
Volume Imaging ◽  
Freely Behaving

The internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here, we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our system through functional imaging of neural activity during visually evoked and prey capture behaviors in larval zebrafish.

scholarly journals Whole-brain calcium imaging during physiological vestibular stimulation in larval zebrafish

2018 ◽  
Author(s):  
Geoffrey Migault ◽  
Thomas Panier ◽  
Raphaël Candelier ◽  
Georges Debrégeas ◽  
Volker Bormuth
Keyword(s):  
Virtual Environments ◽  
Functional Imaging ◽  
Light Sheet ◽  
Vestibular Stimulation ◽  
Brain Response ◽  
Whole Brain ◽  
Larval Zebrafish ◽  
In Vivo Functional Imaging ◽  
First Time

AbstractDuring in vivo functional imaging, animals are head-fixed and thus deprived from vestibular inputs, which severely hampers the design of naturalistic virtual environments. To overcome this limitation, we developed a miniaturized ultra-stable light-sheet microscope that can be dynamically rotated during imaging along with a head-restrained zebrafish larva. We demonstrate that this system enables whole-brain functional imaging at single-cell resolution under controlled vestibular stimulation. We recorded for the first time the dynamic whole-brain response of a vertebrate to physiological vestibular stimulation. This development largely expands the potential of virtual-reality systems to explore complex multisensory-motor integration in 3D.

scholarly journals Neural dysfunction at the upper thermal limit in the zebrafish

2020 ◽  
Author(s):  
Anna H. Andreassen ◽  
Petter Hall ◽  
Pouya Khatibzadeh ◽  
Fredrik Jutfelt ◽  
Florence Kermen
Keyword(s):  
Heart Rate ◽  
Heat Stress ◽  
High Temperatures ◽  
Neural Activity ◽  
Thermal Tolerance ◽  
Oxygen Availability ◽  
Adult Zebrafish ◽  
Thermal Limit ◽  
Larval Zebrafish ◽  
Upper Thermal Limit

ABSTRACTUnderstanding animal thermal tolerance is crucial to predict how animals will respond to increasingly warmer temperatures, and to mitigate the impact of the climate change on species survival. Yet, the physiological mechanisms underlying animal thermal tolerance are largely unknown. In this study, we developed a method for measuring upper thermal limit (CTmax) in larval zebrafish (Danio rerio) and found that it occurs at similar temperatures as in adult zebrafish. We discovered that CTmax precedes a transient, heat-induced brain-wide depolarization during heat ramping. By monitoring heart rate, we established that cardiac function is sub-optimal during the period where CTmax and brain depolarization occur. In addition, we found that oxygen availability affects both locomotor neural activity and CTmax during a heat stress. The findings of this study suggest that neural impairment due to limited oxygen availability at high temperatures can cause CTmax in zebrafish.HighlightsLarval zebrafish reach their critical thermal limit (CTmax) at similar temperature as adult zebrafishAcute heat stress causes a brain-wide spreading depolarization near the upper thermal limitCTmax precedes brain-wide depolarizationHeart rate declines at high temperatures but is maintained during CTmax and brain depolarizationNeural activity is impaired prior to CTmax and brain-wide depolarizationOxygen availability in the water affects both CTmax and neural activity

scholarly journals Noninvasive recording of the Mauthner neurone action potential in larval zebrafish

1982 ◽  
Vol 101 (1) ◽  
pp. 83-92 ◽  
Author(s):  
J. I. Prugh ◽  
C. B. Kimmel ◽  
W. K. Metcalfe
Keyword(s):  
Neural Activity ◽  
Action Potentials ◽  
Cell Function ◽  
Recording Site ◽  
M Cell ◽  
Larval Zebrafish ◽  
System 2 ◽  
The Central Nervous System ◽  
Freely Behaving ◽  
M Spike

We describe the identification of Mauthner (M-) cell action potentials in an intact zebrafish larva, utilizing recording electrodes located outside the fish: 1. The externally recorded spike occurs at approximately the same time, and its waveform changes with recording site in the same way, as the extracellular M-spike recorded within the central nervous system. 2. The externally recorded M-spike may be readily distinguished from other forms of neural activity. 3. The M-spike can be identified in recordings from unrestrained larvae. This finding permits the direct study of M-cell function in the freely behaving animal.

scholarly journals A brainstem integrator for self-localization and positional homeostasis

2021 ◽  
Author(s):  
En Yang ◽  
Maarten F Zwart ◽  
Mikail Rubinov ◽  
Ben James ◽  
Ziqiang Wei ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Optic Flow ◽  
Inferior Olive ◽  
Brain Regions ◽  
Neural Pathway ◽  
Whole Brain ◽  
Water Currents ◽  
Larval Zebrafish ◽  
Integrate Optic ◽  
As If

To accurately track self-location, animals need to integrate their movements through space. In amniotes, representations of self-location have been found in regions such as the hippocampus. It is unknown whether more ancient brain regions contain such representations and by which pathways they may drive locomotion. Fish displaced by water currents must prevent uncontrolled drift to potentially dangerous areas. We found that larval zebrafish track such movements and can later swim back to their earlier location. Whole-brain functional imaging revealed the circuit enabling this process of positional homeostasis. Position-encoding brainstem neurons integrate optic flow, then bias future swimming to correct for past displacements by modulating inferior olive and cerebellar activity. Manipulation of position-encoding or olivary neurons abolished positional homeostasis or evoked behavior as if animals had experienced positional shifts. These results reveal a multiregional hindbrain circuit in vertebrates for optic flow integration, memory of self-location, and its neural pathway to behavior.

scholarly journals Whole-brain serial-section electron microscopy in larval zebrafish

2017 ◽  
Author(s):  
David Grant Colburn Hildebrand ◽  
Marcelo Cicconet ◽  
Russel Miguel Torres ◽  
Woohyuk Choi ◽  
Tran Minh Quan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Serial Section ◽  
Acquisition Time ◽  
Myelinated Axons ◽  
Whole Brain ◽  
Larval Zebrafish ◽  
Section Electron ◽  
Serial Section Electron Microscopy ◽  
Section Electron Microscopy

Investigating the dense meshwork of wires and synapses that form neuronal circuits is possible with the high resolution of serial-section electron microscopy (ssEM)1. However, the imaging scale required to comprehensively reconstruct axons and dendrites is more than 10 orders of magnitude smaller than the spatial extents occupied by networks of interconnected neurons2—some of which span nearly the entire brain. The difficulties in generating and handling data for relatively large volumes at nanoscale resolution has thus restricted all studies in vertebrates to neuron fragments, thereby hindering investigations of complete circuits. These efforts were transformed by recent advances in computing, sample handling, and imaging techniques1, but examining entire brains at high resolution remains a challenge. Here we present ssEM data for a complete 5.5 days post-fertilisation larval zebrafish brain. Our approach utilizes multiple rounds of targeted imaging at different scales to reduce acquisition time and data management. The resulting dataset can be analysed to reconstruct neuronal processes, allowing us to, for example, survey all the myelinated axons (the projectome). Further, our reconstructions enabled us to investigate the precise projections of neurons and their contralateral counterparts. In particular, we observed that myelinated axons of reticulospinal and lateral line afferent neurons exhibit remarkable bilateral symmetry. Additionally, we found that fasciculated reticulospinal axons maintain the same neighbour relations throughout the extent of their projections. Furthermore, we use the dataset to set the stage for whole-brain comparisons of structure and function by co-registering functional reference atlases and in vivo two-photon fluorescence microscopy data from the same specimen. We provide the complete dataset and reconstructions as an open-access resource for neurobiologists and others interested in the ultrastructure of the larval zebrafish.

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