scholarly journals Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Ying Wang ◽  
Thomas S Chaffee ◽  
Rebecca S LaRue ◽  
Danielle N Huggins ◽  
Patrice M Witschen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Mammary Gland ◽  
Mammary Gland Development ◽  
Close Association ◽  
Transcriptional Profiling ◽  
Ecm Remodeling ◽  
Macrophage Depletion ◽  
Single Cell Rna Sequencing ◽  
Gland Development ◽  
Ductal Development

Tissue-resident macrophages in the mammary gland are found in close association with epithelial structures and within the adipose stroma, and are important for mammary gland development and tissue homeostasis. Macrophages have been linked to ductal development in the virgin mammary gland, but less is known regarding the effects of macrophages on the adipose stroma. Using transcriptional profiling and single-cell RNA sequencing approaches, we identify a distinct resident stromal macrophage subpopulation within the mouse nulliparous mammary gland that is characterized by the expression of Lyve-1, a receptor for the extracellular matrix (ECM) component hyaluronan. This subpopulation is enriched in genes associated with ECM remodeling and is specifically associated with hyaluronan-rich regions within the adipose stroma and fibrous capsule of the virgin mammary gland. Furthermore, macrophage depletion leads to enhanced accumulation of hyaluronan-associated ECM in the adipose-associated stroma, indicating that resident macrophages are important for maintaining homeostasis within the nulliparous mammary gland stroma.

scholarly journals The Role of Csmd1 during Mammary Gland Development

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 162
Author(s):  
Samuel J. Burgess ◽  
Hannah Gibbs ◽  
Carmel Toomes ◽  
Patricia L. Coletta ◽  
Sandra M. Bell
Keyword(s):  
Mammary Gland ◽  
Mammary Gland Development ◽  
Expression Patterns ◽  
Development In Vitro ◽  
Duct Development ◽  
Gland Development ◽  
Ductal Development

The Cub Sushi Multiple Domains-1 (CSMD1) protein is a tumour suppressor which has been shown to play a role in regulating human mammary duct development in vitro. CSMD1 knockdown in vitro demonstrated increased cell proliferation, invasion and motility. However, the role of Csmd1 in vivo is poorly characterised when it comes to ductal development and is therefore an area which warrants further exploration. In this study a Csmd1 knockout (KO) mouse model was used to identify the role of Csmd1 in regulating mammary gland development during puberty. Changes in duct development and protein expression patterns were analysed by immunohistochemistry. This study identified increased ductal development during the early stages of puberty in the KO mice, characterised by increased ductal area and terminal end bud number at 6 weeks. Furthermore, increased expression of various proteins (Stat1, Fak, Akt, Slug/Snail and Progesterone receptor) was shown at 4 weeks in the KO mice, followed by lower expression levels from 6 weeks in the KO mice compared to the wild type mice. This study identifies a novel role for Csmd1 in mammary gland development, with Csmd1 KO causing significantly more rapid mammary gland development, suggesting an earlier adult mammary gland formation.

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