scholarly journals Detection and variability analyses of CRISPR-like loci in theH. pylorigenome

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6221
Author(s):  
Jerson Alexander García-Zea ◽  
Roberto de la Herrán ◽  
Francisca Robles Rodríguez ◽  
Rafael Navajas-Pérez ◽  
Carmelo Ruiz Rejón
Keyword(s):  
Helicobacter Pylori ◽  
Geographical Area ◽  
Pathogenic Bacterium ◽  
Gene Location ◽  
Phylogenetic Marker ◽  
Genomic Plasticity ◽  
Human Pathogenic Bacterium ◽  
Different Strains ◽  
Genomic Locations

Helicobacter pyloriis a human pathogenic bacterium with a high genomic plasticity. Although the functional CRISPR-Cas system has not been found in its genome, CRISPR-like loci have been recently identified. In this work, 53 genomes from different geographical areas are analyzed for the search and analysis of variability of this type of structure. We confirm the presence of a locus that was previously described in the VlpC gene in al lgenomes, and we characterize new CRISPR-like loci in other genomic locations. By studying the variability and gene location of these loci, the evolution and the possible roles of these sequences are discussed. Additionally, the usefulness of this type of sequences as a phylogenetic marker has been demonstrated, associating the different strains by geographical area.

scholarly journals Detection and variability analyses of CRISPR-like loci in the H. pylori genome

2018 ◽  
Author(s):  
Jerson Alexander Garcia-Zea ◽  
Roberto de la Herrán ◽  
Francisca Robles Rodríguez ◽  
Rafael Navajas-Pérez ◽  
Carmelo Ruiz Rejón
Keyword(s):  
Helicobacter Pylori ◽  
Geographical Area ◽  
Pathogenic Bacterium ◽  
Gene Location ◽  
Phylogenetic Marker ◽  
Genomic Plasticity ◽  
Human Pathogenic Bacterium ◽  
H Pylori ◽  
Different Strains ◽  
Genomic Locations

Helicobacter pylori is a human pathogenic bacterium with a high genomic plasticity. Although the functional CRISPR-Cas system has not been found in its genome, CRISPR like loci have been recently identified. In this work, 53 genomes from different geographical areas are analyzed for the search and analysis of variability of this type of structure. We confirm the presence of a locus that was previously described in the VlpC gene in al lgenomes, and we characterize new CRISPR-like loci in other genomic locations. By studying the variability and gene location of these loci, the evolution and the possible roles of these sequences are discussed. Additionally, the usefulness of this type of sequences as a phylogenetic marker has been demonstrated, associating the different strains by geographical area.

scholarly journals Detection and variability analyses of CRISPR-like loci in the H. pylori genome

2018 ◽  
Author(s):  
Jerson Alexander Garcia-Zea ◽  
Roberto de la Herrán ◽  
Francisca Robles Rodríguez ◽  
Rafael Navajas-Pérez ◽  
Carmelo Ruiz Rejón
Keyword(s):  
Helicobacter Pylori ◽  
Geographical Area ◽  
Pathogenic Bacterium ◽  
Gene Location ◽  
Phylogenetic Marker ◽  
Genomic Plasticity ◽  
Human Pathogenic Bacterium ◽  
H Pylori ◽  
Different Strains ◽  
Genomic Locations

Helicobacter pylori is a human pathogenic bacterium with a high genomic plasticity. Although the functional CRISPR-Cas system has not been found in its genome, CRISPR like loci have been recently identified. In this work, 53 genomes from different geographical areas are analyzed for the search and analysis of variability of this type of structure. We confirm the presence of a locus that was previously described in the VlpC gene in al lgenomes, and we characterize new CRISPR-like loci in other genomic locations. By studying the variability and gene location of these loci, the evolution and the possible roles of these sequences are discussed. Additionally, the usefulness of this type of sequences as a phylogenetic marker has been demonstrated, associating the different strains by geographical area.

Molecular cloning and characterization of a new peptide deformylase from human pathogenic bacterium Helicobacter pylori

2004 ◽  
Vol 319 (4) ◽  
pp. 1292-1298 ◽  
Author(s):  
Cong Han ◽  
Qi Wang ◽  
Lei Dong ◽  
Haifang Sun ◽  
Shuying Peng ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Molecular Cloning ◽  
Pathogenic Bacterium ◽  
Peptide Deformylase ◽  
Human Pathogenic Bacterium

scholarly journals Pathogenic bacterium Helicobacter pylori alters the expression profile of p53 protein isoforms and p53 response to cellular stresses

2012 ◽  
Vol 109 (38) ◽  
pp. E2543-E2550 ◽  
Author(s):  
Jinxiong Wei ◽  
Jennifer Noto ◽  
Elena Zaika ◽  
Judith Romero-Gallo ◽  
Pelayo Correa ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Expression Profile ◽  
P53 Protein ◽  
Pathogenic Bacterium ◽  
Protein Isoforms ◽  
Cellular Damage ◽  
Mongolian Gerbils ◽  
Cellular Stresses ◽  
H Pylori ◽  
P53 Isoforms

The p53 protein plays a central role in the prevention of tumorigenesis. Cellular stresses, such as DNA damage and aberrant oncogene activation, trigger induction of p53 that halts cellular proliferation and allows cells to be repaired. If cellular damage is beyond the capability of the repair mechanisms, p53 induces apoptosis or cell cycle arrest, preventing damaged cells from becoming cancerous. However, emerging evidence suggests that the function of p53 needs to be considered as isoform-specific. Here, we report that the expression profile of p53 can be shifted toward inhibitory p53 isoforms by the pathogenic bacterium Helicobacter pylori, which is known for its strong association with gastric cancer and gastric mucosa-associated lymphoid tissue lymphoma. We found that interaction of H. pylori with gastric epithelial cells, mediated via the cag pathogenicity island, induces N-terminally truncated ∆133p53 and ∆160p53 isoforms in human cells. Induction of an orthologous p53 isoform, ∆153p53, was also found in H. pylori-infected Mongolian gerbils. The p53 isoforms inhibit p53 and p73 activities, induce NF-κB, and increase survival of infected cells. Expression of ∆133p53, in response to H. pylori infection, is regulated by phosphorylation of c-Jun and activation of activator protein-1–dependent transcription. Together, these results provide unique insights into the regulation of p53 protein and may contribute to the understanding of tumorigenesis associated with H. pylori.

scholarly journals Genotypic Profile of the Outer Membrane Proteins BabA and BabB in Clinical Isolates of Helicobacter pylori

2006 ◽  
Vol 74 (7) ◽  
pp. 4375-4378 ◽  
Author(s):  
Jeffrey C. Colbeck ◽  
Lori M. Hansen ◽  
Julie M. Fong ◽  
Jay V. Solnick
Keyword(s):  
Helicobacter Pylori ◽  
Outer Membrane Proteins ◽  
Genotypic Diversity ◽  
Blood Group Antigen ◽  
Antigen Binding ◽  
Abo Blood Group ◽  
Selective Pressures ◽  
Different Strains ◽  
Genotypic Profile ◽  
Over Time

ABSTRACT Helicobacter pylori BabA is the ABO blood group antigen binding adhesin, which has a closely related paralogue (BabB) whose function is unknown. PCR and DNA sequence analysis showed extensive genotypic diversity in babA and babB across different strains, as well as within a strain colonizing an individual patient. We hypothesize that diverse profiles of babA and babB reflect selective pressures for adhesion, which may differ across different hosts and within an individual over time.

Biosynthesis of copper nanoparticles; its characterization and efficacy against human pathogenic bacterium

2016 ◽  
Vol 4 (2) ◽  
pp. 2163-2169 ◽  
Author(s):  
B.H. Patel ◽  
M.Z. Channiwala ◽  
S.B. Chaudhari ◽  
A.A. Mandot
Keyword(s):  
Copper Nanoparticles ◽  
Pathogenic Bacterium ◽  
Human Pathogenic Bacterium

Green Synthesis of Silver Nano-Sols by Leaf Extract of Ocimum sanctum and Their Efficacy Against Human Pathogenic Bacterium

2015 ◽  
Vol 2 (1) ◽  
pp. 39-44 ◽  
Author(s):  
B. H. Patel ◽  
M. Z. Channiwala ◽  
S. B. Chaudhari ◽  
. A. A. Mandot
Keyword(s):  
Green Synthesis ◽  
Leaf Extract ◽  
Pathogenic Bacterium ◽  
Ocimum Sanctum ◽  
Human Pathogenic Bacterium

scholarly journals Serum Antibody Responses to Helicobacter pylori and the cagA Marker in Patients with Mucosa-Associated Lymphoid Tissue Lymphoma

1999 ◽  
Vol 6 (4) ◽  
pp. 633-638 ◽  
Author(s):  
Anne Taupin ◽  
Alessandra Occhialini ◽  
Agnès Ruskone-Fourmestraux ◽  
Jean-Charles Delchier ◽  
Jean-Claude Rambaud ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibody Response ◽  
Malt Lymphoma ◽  
Lymphoid Tissue ◽  
Serum Antibody ◽  
Dot Blot ◽  
Homologous Strain ◽  
H Pylori ◽  
Different Strains ◽  
Dot Blot Analysis

ABSTRACT The lymphoma of the mucosa-associated lymphoid tissue (MALT) of the stomach has been linked to Helicobacter pylori infection, but the mechanisms involved in B-cell proliferation remain elusive. In a search for putative H. pylori-specific monoclonal immunoglobulin production, an H. pylori strain was isolated from 10 patients with MALT lymphoma and used to detect the specific serum antibody response to the homologous strain by immunoblotting. Moreover, the antigenicity of the different strains was compared by using each of the 10 sera. We found that the different strains induced highly variable patterns of systemic immunoglobulin G antibody response, although several bacterial antigens, such as the 60-kDa urease B, were often recognized by the different sera. ThecagA marker was detected in the strains by PCR with specific primers and by dot blot analysis, and the CagA protein was found in the sera of 4 of the 10 patients by immunoblotting. In conclusion, MALT lymphoma patients, like other patients with H. pylori gastritis, exhibit a polymorphic systemic antibody response, despite an apparently similar antigenic profile. The CagA marker of pathogenicity is not associated with this disease.

Evaluation of Apoptosis Induction Using PARP Cleavage on Gastric Adenocarcinoma and Fibroblast Cell Lines by Different Strains of Helicobacter pylori

2007 ◽  
Vol 10 (22) ◽  
pp. 4097-4102 ◽  
Author(s):  
Ali Mojtahedi ◽  
Rasoul Salehi ◽  
Farahtaj Navabakbar ◽  
Hasan Tamizifar ◽  
Hamid tavakoli ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Adenocarcinoma ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Parp Cleavage ◽  
Apoptosis Induction ◽  
Different Strains ◽  
Fibroblast Cell Lines
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