Prevalence of Campylobacter spp., Salmonella spp., Listeria monocytogenes, and Hepatitis E Virus in Swine Livers Collected at an Abattoir

A study of imported unprepared whole lettuces sampled from supermarkets, greengrocers, shops, and market stalls found that all were of acceptable microbiological quality. Twenty-seven out of 151 (18%) imported lettuce samples had Enterobacteriaceae levels of 104 CFU/g or more. However, these bacteria that constitute part of the natural microflora of unprepared vegetables may also be derived from the soil and/or by poor handling. The pathogens, Salmonella spp., Shigella spp., Campylobacter spp., Escherichia coli O157:H7, Vibrio cholerae, Listeria monocytogenes, and also Escherichia coli, an indicator of fecal contamination, were not detected in any imported lettuces, indicating that hygiene, harvesting, and production practices were good. Imported lettuces with Enterobacteriaceae levels of 104 CFU/g or more varied with type of retail premises and the temperature at which the lettuces were displayed. Samples from greengrocers, shops, and market stalls were more likely to contain Enterobacteriaceae at levels in excess of 104 CFU/g than those from supermarkets.

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Saúde Animal, Biossegurança e Segurança dos alimentos

Archivos de Zootecnia ◽

10.21071/az.v67isupplement.3589 ◽

2018 ◽

Vol 67 (Supplement) ◽

pp. 131-135

Author(s):

R. Potes ◽

M.A. Laranjo ◽

L. Elias

Keyword(s):

Listeria Monocytogenes ◽

Toxoplasma Gondii ◽

Yersinia Enterocolitica ◽

Trichinella Spiralis ◽

Taenia Solium ◽

Salmonella Spp ◽

Campylobacter Spp

Alguns perigos que podem ameaçar a saúde dos consumidores de carne de porco e de produtos processados estão relacionados com a saúde animal ou com a manipulação da matéria-prima desde o abate dos animais, a transformação e até ao consumo. Entre os perigos químicos consideram-se as substâncias com efeitos anabólicos, os resíduos de substâncias medicamentosas e os contaminantes químicos, alguns com origem na produção animal ou produzidos durante o processamento. No que se refere aos perigos biológicos, podem encontrar-se alguns parasitas como Toxoplasma gondii, Trichinella spiralis e Taenia solium e também bactérias como Salmonella spp, Campylobacter spp., Yersinia enterocolitica, Listeria monocytogenes. As características físico-químicas da carne fresca facilitam o crescimento de diversos microrganismos, para além dos mencionados anteriormente, o que a torna altamente perecível. Os diferentes processos de transformação desta matéria-prima, não só permitem obter diferentes alimentos para o consumidor como constituem formas de aumentar a sua vida útil. Entre os processos de transformação incluem-se a secagem, a fermentação e a cura. Em Portugal há uma grande variedade de produtos cárneos tais como Chouriço, Paio, Presunto, Salsichão e Catalão, muitos dos quais são fabricados segundo as técnicas tradicionais das diferentes regiões de onde são originários. Alguns destes produtos têm sido estudados na Universidade de Évora. Apresentaremos alguns resultados que mostram o efeito favorável de vários processos de transformação no controlo de alguns dos perigos biológicos mencionados.

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Occurrence of Zoonotic Bacteria in Retail Game Meat in Japan with Special Reference to Erysipelothrix

Journal of Food Protection ◽

10.4315/0362-028x-60.3.328 ◽

1997 ◽

Vol 60 (3) ◽

pp. 328-331 ◽

Cited By ~ 20

Author(s):

YOKO KANAI ◽

HIDEKI HAYASHIDANI ◽

KEN-ICHI KANEKO ◽

MASUO OGAWA ◽

TOSHIO TAKAHASHI ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Wild Boar ◽

Yersinia Enterocolitica ◽

The Other ◽

Salmonella Spp ◽

Serotype 1 ◽

Meat Samples ◽

Zoonotic Bacteria ◽

Highly Virulent ◽

Campylobacter Spp

From December 1993 to March 1994, a total of 93 wild boar and 30 deer meat samples were purchased from 13 retail shops and examined for the presence of species of Erysipelothrix, Yersinia, Listeria, Salmonella and Campylobacter. Erysipelothrix spp. were isolated from 41 wild boar and 15 deer samples. These isolates were identified as 13 serotypes. Serotypes 6 and 2 were the most predominant. Of 17 isolates examined, 14 isolates were highly virulent for mice. The bacteria of the same serotype were isolated repeatedly from the samples purchased in 4 of the shops. Yersinia enterocolitica was isolated from 36 wild boar and 10 deer samples; however, all isolates of Y. enterocolitica did not have pathogenic properties. Listeria monocytogenes was isolated from 5 wild boar samples. The isolates were identified as serotype 1/2c and 4b. Salmonella spp. were isolated from 2 wild boar samples; one isolate was identified as S. typhimurium and the other was untypable. Campylobacter spp. were not detected.

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Epidemiología de Salmonella spp., Listeria monocytogenes y Campylobacter spp., en la cadena productiva avícola

IATREIA ◽

10.17533/udea.iatreia.v29n4a01 ◽

2016 ◽

Vol 29 (4) ◽

Author(s):

ME Realpe-Delgado ◽

◽

ÁB Muñoz-Delgado ◽

P Donado-Godoy ◽

LM Rey-Ramírez ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Salmonella Spp ◽

Campylobacter Spp

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Evaluation of oral fluids for surveillance of foodborne and zoonotic pathogens in pig farms

Journal of Veterinary Diagnostic Investigation ◽

10.1177/10406387211021599 ◽

2021 ◽

pp. 104063872110215

Author(s):

Franziska Schott ◽

Karolin Hoffmann ◽

Eleonora Sarno ◽

Patrick D. Bangerter ◽

Roger Stephan ◽

...

Keyword(s):

Hepatitis E Virus ◽

Hepatitis E ◽

Serum Samples ◽

Salmonella Spp ◽

Zoonotic Pathogens ◽

Oral Fluids ◽

Mcnemar Test ◽

Nasal Swabs ◽

Pig Farms ◽

Sampling Times

The use of oral fluid (OF) to detect zoonotic pathogens in pigs has been only scarcely assessed. We evaluated OF as a potential specimen for detection by culture of methicillin-resistant Staphylococcus aureus (MRSA) and Yersinia enterocolitica, and the detection of antibodies against Salmonella spp. and hepatitis E virus (HEV) using commercial ELISAs. Samples from 33 pig farms were collected at the beginning and end of the fattening period. Results of the OF samples were compared with the results of serum samples and nasal swabs from individual pigs and pen floor fecal samples, using the Cohen kappa (κ) and the McNemar test. For Salmonella spp. antibodies, OF samples were negative, although the corresponding serum samples were positive. The detection of HEV antibodies in sera and OF had agreement at the first sampling, and poor and significant agreement at the second sampling (κ = 0.185, McNemar p = 0.238; κ = 0.088, McNemar p < 0.001). At both sampling times, the detection of MRSA in nasal swabs and OF showed agreement (κ = 0.466, McNemar p = 0.077; κ = 0.603, McNemar p = 1); agreement was seen for the detection of Y. enterocolitica in fecal and OF samples (κ = 0.012, McNemar p = 0.868; κ = 0.082, McNemar p = 0.061, respectively). According to the McNemar test, the use of pen-based OFs is more feasible for the detection of MRSA and Y. enterocolitica by culture than is detection of antibodies by commercial ELISA.

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QUALIDADE MICROBIOLÓGICA DO QUEIJO MINAS FRESCAL COMERCIALIZADO NO MUNICÍPIO DE JABOTICABAL, SP, BRASIL

Arquivos do Instituto Biológico ◽

10.1590/1808-1657v73p1712006 ◽

2006 ◽

Vol 73 (2) ◽

pp. 171-175

Author(s):

B.M. Salotti ◽

A.C.F.B. Carvalho ◽

L.A. Amaral ◽

A.M.C. Vidal-Martins ◽

A.L. Cortez

Keyword(s):

Listeria Monocytogenes ◽

Sao Paulo ◽

São Paulo ◽

Salmonella Spp ◽

Campylobacter Spp

RESUMO No período de julho a dezembro de 2002, foi avaliada a qualidade microbiológica de 60 amostras de queijo minas frescal, através do Número Mais Provável (NMP) de coliformes fecais, contagem de Staphylococcus coagulase positiva, pesquisa de Salmonella spp., pesquisa de Listeria monocytogenes e pesquisa de Campylobacter spp., sendo que, 30 amostras eram de produção artesanal e 30 de produção industrial fiscalizadas pelo Serviço de Inspeção Estadual e Federal e foram adquiridas no comércio do Município de Jaboticabal, São Paulo. Quanto a presença de coliformes fecais, apresentaram-se em desacordo com o estabelecido pela Agência Nacional de Vigilância Sanitária (ANVISA) 83,4% das amostras artesanais e para as amostras industriais 66,7%. Para os valores obtidos na contagem de Staphylococcus coagulase positiva, 20% das amostras artesanais e 10% das amostras industriais, apresentaram-se em desacordo com o estabelecido pela Anvisa. Com relação a detecção de Salmonella spp., Listeria monocytogenes e Campylobacter spp., tais microrganismos não foram isolados em 25 g do produto, mostrando que em relação a estes agentes os queijos analisados tanto de produção artesanal como os industrializados, apresentaram-se dentro dos padrões estabelecidos (ausência em 25 g do produto). Os resultados microbiológicos revelaram que 86,7% das amostras artesanais e 66,7% das amostras industriais encontravam-se em desacordo com os padrões estabelecidos pela Anvisa, segundo a Resolução n° 12 de 02 de janeiro de 2001. Tais resultados demonstram o risco potencial que este produto pode representar para a saúde da população consumidora.

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Biofilm bakteryjny i możliwości jego eliminacji w przemyśle spożywczym

Zywnosc Nauka Technologia Jakosc/Food Science Technology Quality ◽

10.15193/zntj/2021/127/374 ◽

2021 ◽

Vol 127 (2) ◽

pp. 5-20

Author(s):

Kamila Joanna Daniluk ◽

Michał Wójcicki ◽

Edyta Juszczuk-Kubiak

Keyword(s):

Escherichia Coli ◽

Listeria Monocytogenes ◽

Salmonella Spp ◽

Campylobacter Spp

Biofilmy bakteryjne występujące w przemyśle spożywczym stanowią złożone, wielogatunkowe grupy zarówno bakterii saprofitycznych, jak i patogennych. Często zlokalizowane są na powierzchniach trudno dostępnych dla środków myjących stosowanych w obiegu zamkniętym, jak i dla mechanicznego czyszczenia. W przemyśle spożywczym biofilmy bakteryjne stanowią źródło zanieczyszczeń mikrobiologicznych żywności, powodując obniżenie jakości i trwałości produktów spożywczych. Ponadto biofilmy spożywcze mogą być źródłem bakterii patogennych, takich jak Salmonella spp., Campylobacter spp. czy Listeria monocytogenes oraz bakterii oportunistycznych (np. Escherichia coli), wywołujących choroby układu pokarmowego, które mogą być długotrwałe i trudne do leczenia, zwłaszcza u osób z obniżoną odpornością. W niniejszym opracowaniu przedstawiono podstawowe mechanizmy tworzenia biofilmu bakteryjnego oraz najistotniejsze funkcje pełnione przez zewnątrzkomórkową macierz biofilmu (EPS). Scharakteryzowano główne bakteryjne patogeny występujące w branżach: mięsnej, mleczarskiej, ryb i owoców morza, produktów pochodzenia roślinnego o minimalnym stopniu przetworzenia oraz w sokowniczej, z uwzględnieniem możliwości tworzenia przez te drobnoustroje biofilmu trwałego i opornego na czynniki zewnętrzne. Przedyskutowano również możliwości eradykacji biofilmów spożywczych ze szczególnym uwzględnieniem metod polegających na zastosowaniu związków naturalnych pochodzenia roślinnego oraz wykorzystaniu litycznych bakteriofagów i/lub ich oczyszczonych enzymów.

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Prévalence de Salmonella, Listeria monocytogenes, Campylobacter spp., Yersinia enterocolitica et Cryptosporidium spp. dans du lait en vrac, des matières fécales de vaches et des effluents de fermes laitières à Trinidad

Revue d’élevage et de médecine vétérinaire des pays tropicaux ◽

10.19182/remvt.9501 ◽

1996 ◽

Vol 49 (4) ◽

pp. 303-309

Author(s):

A.A. Adesiyun ◽

L.A. Webb ◽

H. Romain ◽

J.S. Kaminjolo

Keyword(s):

Listeria Monocytogenes ◽

Yersinia Enterocolitica ◽

Salmonella Spp ◽

Cryptosporidium Spp ◽

Campylobacter Spp

La prévalence de Listeria, Salmonella, Campylobacter, Yersinia et Cryptosporidium dans du lait en vrac, les matières fécales des vaches laitières et des effluents à lait des fermes laitières de l'île de la Trinité, a été evaluée. Des 177 échantillons de lait en vrac testés, 3 (1,7 %), 3 (1,7 %) et 2 (1,2 %) étaient positifs respectivement pour Listeria monocytogenes, Salmonella spp. et Yersinia enterocolitica mais négatifs pour Campylobacter spp. et Cryptosporidium spp. Des 333 échantillons fécaux testés, L. monocytogenes, Campylobacter spp. et Cryptosporidium spp. étaient présents dans respectivement 11 (3,3 %), 19 (5,7 %) et 7 (2,1 %) des cas. Ces taux étaient significativement (P < 0,05; chi2) plus élevés, que le taux de détection (0 %) pour Yersinia spp. Salmonella spp. a été detectée dans 3 (0,9 %) échantillons fécaux. Des 168 échantillons prélevés des effluents à lait, Campylobacter spp. était présent dans 7 (4,2 %) échantillons alors que Salmonella spp. l'était dans seulement 1 (0,6 %) échantillon. Aucun échantillon n'était positif pour Yersinia spp. et Listeria spp. (différence significative, P<= 0,05; chi2). En conclusion, les cinq espèces entero-pathogènes testées étaient présentes dans les fermes laitières de l'île de la Trinité.

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