Epidemiological Characteristics and Clinical Treatment Outcome of Typhoid Fever in Ningbo, China, 2005–2014: Pulsed-Field Gel Electorophoresis Results Revealing Great Proportion of Common Transmission Sources

Salmonella enterica serovar Bareilly (S. Bareilly) has been among the top 20 most frequently isolated serovars in the United States and has been observed recently in layer flocks in Korea. Between 2013 and 2014, 45 S. Bareilly isolates were obtained from five commercial layer farms and nine retail markets in Korea. Among the 45 isolates, four pulsed-field gel electrophoresis patterns were observed, with pattern B being the predominant and comprising 67% of the 45 isolates. The most common antimicrobial resistance was streptomycin (24.4%) and cephalothin (6.7%). This is the first report describing epidemiological characteristics of S. Bareilly, including geographical variation, in Korea.

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CLINICAL AND EPIDEMIOLOGICAL CHARACTERISTICS OF ACUTE CORONARY SYNDROME AND TREATMENT OUTCOME IN THE JABLANICA DISTRICT

Acta Medica Medianae ◽

10.5633/amm.2017.0102 ◽

2017 ◽

Vol 56 (1) ◽

pp. 9-16

Author(s):

Goran Cvetanović ◽

◽

Marija Stojiljković ◽

Nebojša Dimitrijević ◽

Jasmina Ranđelović

Keyword(s):

Acute Coronary Syndrome ◽

Treatment Outcome ◽

Coronary Syndrome ◽

Epidemiological Characteristics

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Treatment outcome with implant-retained overdentures: Part I—Clinical findings and predictability of clinical treatment outcome

Journal of Prosthetic Dentistry ◽

10.1016/0022-3913(94)90072-8 ◽

1994 ◽

Vol 72 (2) ◽

pp. 144-151 ◽

Cited By ~ 31

Author(s):

Marco S. Cune ◽

Cornelis de Putter ◽

Johan Hoogstraten

Keyword(s):

Treatment Outcome ◽

Clinical Findings ◽

Clinical Treatment

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Epidemiological Study of a Food-Borne Outbreak of EnterotoxigenicEscherichia coli O25:NM by Pulsed-Field Gel Electrophoresis and Randomly Amplified Polymorphic DNA Analysis

Journal of Clinical Microbiology ◽

10.1128/jcm.36.3.652-656.1998 ◽

1998 ◽

Vol 36 (3) ◽

pp. 652-656 ◽

Cited By ~ 20

Author(s):

Toshihiro Mitsuda ◽

Tetsunori Muto ◽

Mikiko Yamada ◽

Nobuyoshi Kobayashi ◽

Masanori Toba ◽

...

Keyword(s):

Gel Electrophoresis ◽

Dna Analysis ◽

Pulsed Field Gel Electrophoresis ◽

Causal Agent ◽

Randomly Amplified Polymorphic Dna ◽

Pulsed Field ◽

Food Borne ◽

Length Polymorphisms ◽

Genotypic Analysis ◽

Epidemiological Characteristics

This study investigated the applicability of molecular epidemiological techniques to the identification of the causal agent of an outbreak of diarrhea caused by ingestion of food contaminated with enterotoxigenic Escherichia coli (ETEC). The outbreak occurred at four elementary schools in July 1996 and affected more than 800 people. Illness was most strongly associated with eating tuna paste (relative risk, 1.79; 95% confidence interval = 1.16 to 2.79; P = 0.0001). To evaluate the epidemiological characteristics of the pathogen, the DNAs from numerous isolated ETEC strains were subjected to randomly amplified polymorphic DNA analysis, pulsed-field gel electrophoresis of nuclease S1-treated plasmid DNA, and analysis of genomic DNA restriction fragment length polymorphisms. All ETEC isolates were of the O25:NM (nonmotile) serotype, which carries a heat-stable enterotoxin Ib gene. Genotypic analysis demonstrated that the strains isolated from the patients at all four schools were identical. The isolates of ETEC O25:NM obtained from the tuna paste that had been served for lunch at these schools were genetically indistinguishable from those isolated from the patients. Results suggest that this outbreak was food borne. The molecular biology-based epidemiological techniques used in this study were useful in characterizing the causal agent in this food-borne epidemic.

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