Effect of Biological Control Antagonists Adsorbed on Chitosan Immobilized Silica Nanocomposite on Ralstonia solanacearum and Growth of Tomato Seedlings

2016 ◽  
Vol 6 (3) ◽  
pp. 1-23 ◽  
Author(s):  
Gatahi Dennis ◽  
Wanyika Harrison ◽  
Kihurani Agnes ◽  
Gatebe Erastus
Keyword(s):  
Biological Control ◽  
Ralstonia Solanacearum ◽  
Tomato Seedlings ◽  
Silica Nanocomposite

scholarly journals Streptomyces sp.: Characterization, Identification and Its Potential as a Ralstonia solanacearum Biological Control Agent in vitro

2019 ◽  
Vol 2 (3) ◽  
pp. 89-96 ◽  
Author(s):  
Rachmad Saputra ◽  
Triwidodo Arwiyanto ◽  
Arif Wibowo
Keyword(s):  
Biological Control ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Biological Control Agent ◽  
Wilt Disease ◽  
Molecular Characteristics ◽  
Streptomyces Sp ◽  
Bacterial Wilt Disease ◽  
Wide Range

Streptomyces sp. bacteria have the potential to produce antibiotic compounds, which are one of the mechanisms that are widely used in biological control. However, in general, biological control mechanisms also occur through competition, cell wall degradation and induced resistance. This study was aimed to determine the physiological, biochemical and molecular characteristics of two isolates of Streptomyces sp. (S-4 and S16 isolates) isolated from the tomatoes roots, and to find out their ability to control Ralstonia solanacearum, which causes bacterial wilt disease on a wide range of hosts. The results showed both Streptomyces sp. isolates had several different physiological and biochemical characteristics and had a different ability to inhibit R. solanacearum in vitro. Streptomyces sp. S-16 isolate had a high similarity with Streptomyces diastaticus subsp. ardesiacus strain NRRL B-1773T based on the molecular identification results. Further research needs to be done to see the potential inhibition of the two Streptomyces isolates in inhibiting the development of bacterial wilt disease in tomato plants caused by R. solanacearum.

Ralstonia solanacearum preferential colonization in the shoot apical meristem explains its pathogenicity pattern in tomato seedlings

2020 ◽  
Vol 69 (7) ◽  
pp. 1347-1356
Author(s):  
Kristi Kabyashree ◽  
Rahul Kumar ◽  
Piyali Sen ◽  
Siddhartha S. Satapathy ◽  
Suvendra K. Ray
Keyword(s):  
Ralstonia Solanacearum ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Tomato Seedlings

scholarly journals Biological control of the bacterial wilt Ralstonia solanacearum by bioprotector with fungi chitosan from Cunninghamella elegans on tomatoes

2017 ◽  
Vol 12 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Silva Oliveira Wagner ◽  
Lima Coelho Iwanne ◽  
Rafaella Sousa Oliveira Jessica ◽  
Camila Barros Silva Leite Maria ◽  
Montenegro Stamford Arnaud Thatiana ◽  
...  
Keyword(s):  
Biological Control ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Cunninghamella Elegans

scholarly journals An Innovative Root Inoculation Method to StudyRalstonia solanacearumPathogenicity in Tomato Seedlings

2018 ◽  
Vol 108 (4) ◽  
pp. 436-442 ◽  
Author(s):  
N. Singh ◽  
T. Phukan ◽  
P. L. Sharma ◽  
K. Kabyashree ◽  
A. Barman ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Ralstonia Solanacearum ◽  
Virulence Genes ◽  
Wild Type ◽  
Gus Staining ◽  
Tomato Seedlings ◽  
Inoculation Method ◽  
Virulence Assay ◽  
Bacterial Inoculum ◽  
Adult Plants

In this study, we report Ralstonia solanacearum pathogenicity in the early stages of tomato seedlings by an innovative root inoculation method. Pathogenicity assays were performed under gnotobiotic conditions in microfuge tubes by employing only 6- to 7-day-old tomato seedlings for root inoculation. Tomato seedlings inoculated by this method exhibited the wilted symptom within 48 h and the virulence assay can be completed in 2 weeks. Colonization of the wilted seedlings by R. solanacearum was confirmed by using gus staining as well as fluorescence microscopy. Using this method, mutants in different virulence genes such as hrpB, phcA, and pilT could be clearly distinguished from wild-type R. solanacearum. The method described here is economic in terms of space, labor, and cost as well as the required quantity of bacterial inoculum. Thus, the newly developed assay is an easy and useful approach for investigating virulence functions of the pathogen at the seedling stage of hosts, and infection under these conditions appears to require pathogenicity mechanisms used by the pathogen for infection of adult plants.

Sign in / Sign up

Export Citation Format

Share Document