scholarly journals An Eco-Friendly Production of a Novel and Highly Active Endo-1,4-beta-xylanase from Aspergillus clavatus

2021 ◽  
pp. 20-33
Author(s):  
Thiago Machado Pasin ◽  
Ana Sílvia de Almeida Scarcella ◽  
Rosymar Coutinho de Lucas ◽  
Tássio Brito de Oliveira ◽  
Mariana Cereia ◽  
...  
Keyword(s):  
Molecular Mass ◽  
Paper Industry ◽  
Industrial Applications ◽  
Culture Conditions ◽  
Paper Sludge ◽  
Aspergillus Clavatus ◽  
Industry Waste ◽  
Pulp Industry ◽  
Highly Active ◽  
Paper And Pulp Industry

Aims: Here, we describe a novel way to produce an endo-1,4-beta-xylanase from Aspergillus clavatus using paper and pulp industry waste. Methodology:  Optimal Aspergillus clavatus NRRL1 cultivation conditions were evaluated using minimal medium with different concentrations (1 to 10%) of paper sludge pretreated with HCl, during different periods (1 to 14 days), with different pH values (3.0; 3.5; 4.0; 4.5; 5.0; 5.5; 6.0; 6.5; 7.0; 7.5 and 8.0), different temperatures (25, 30, 35, and 40 ºC) and different mixing conditions (static and stirring). After that, the enzyme activity was determined by DNS (3,5-dinitrosalicylic acid), protein concentration was quantified by Bradford, SDS-PAGE was performed to evaluate the molecular mass, and TLC observed hydrolysis products. Results: The enzyme showed a molecular mass of 25 kDa, and its production has been highly improved by optimizing culture conditions. The best activity of this enzyme was obtained when A. clavatus was cultivated for 5 days, at 120 rpm, 5% paper sludge, pH 6.0, and 35 ºC. The degradation profile of the beechwood xylan by the crude extract containing the GH11 xylanase showed xylotriose as the main product, but xylotetraose and xylobiose were also produced in significant amounts. Conclusion: In addition to the fact that this xylanase has the property of producing large quantities of XOS (mainly xylotriose), it has the advantage of being obtained from recyclable waste of the pulp and paper industry. These facts confer great potential for future biotechnological and industrial applications.

scholarly journals Potential applications of waste lignin from the paper and pulp industry in Viet Nam

2020 ◽  
Vol 23 (4) ◽  
pp. 716-726
Author(s):  
Thanh Khoa Phung ◽  
Quynh-Thy Song Nguyen ◽  
Khanh B. Vu ◽  
Giang Duy-Le Vo ◽  
Vinh Ngoc Nguyen
Keyword(s):  
Paper Industry ◽  
Pulp And Paper Industry ◽  
Forest Tree ◽  
Pulp And Paper ◽  
High Tech ◽  
Pulp Industry ◽  
Potential Applications ◽  
The Government ◽  
Paper And Pulp ◽  
Paper And Pulp Industry

The conversion of waste lignin from the paper and pulp industry is a potential process to produce chemicals and materials in the industry. With the development and the demand for the pulp and paper industry, the amount of waste lignin will increase remarkably. In Vietnam, the forest tree for the pulp industry is abundant, and the pulp industry has increased in recent years. In parallel, the government planned to develop the material resource and high-tech factories for this industry. In this work, we summarized the pulp and paper industry in Vietnam, then suggest the potential applications of waste lignin in several valuable products.

Simultaneous optimization of multiple performance characteristics in coagulation–flocculation process for Indian paper industry wastewater

2012 ◽  
Vol 66 (6) ◽  
pp. 1231-1238 ◽  
Author(s):  
R. Saraswathi ◽  
M. K. Saseetharan
Keyword(s):  
Taguchi Method ◽  
Process Parameters ◽  
Paper Industry ◽  
Oxygen Demand ◽  
Simultaneous Optimization ◽  
Pulp Industry ◽  
Optimization Of Process Parameters ◽  
Paper And Pulp ◽  
Paper And Pulp Industry ◽  
Flocculation Process

The goal of this study was to optimize the coagulation–flocculation process in wastewater generated from the paper and pulp industry using a grey relational analysis (GRA)-based Taguchi method. Process parameters included types and doses of natural coagulants and coagulant aid, and pH. To track the efficiency of the treatment process, the following responses were chosen for optimization: chemical oxygen demand (COD), total dissolved solids (TDS) and turbidity of wastewater, alone or in combination or all together. Analysis of variance showed that the type and dose of the coagulant aid were the most significant parameters, followed by pH and the dose of the coagulant; the type of coagulant used was found to be insignificant in the coagulation–flocculation process. Optimization of process parameters to achieve lower turbidity and greater removal of COD and TDS was verified in a separate confirmatory experiment, which showed improvements in COD and TDS removal and a decrease in turbidity of 8.2, 6.35 and 26.17%, respectively, with the application of the Taguchi method and GRA.

Studies on Paper and Pulp Industry Waste for Leather Making: An Insight in Converting Waste to Wealth

2018 ◽  
pp. 571-581
Author(s):  
P. Balasubramanian ◽  
M. Vedhanayagam ◽  
G. C. Jayakumar ◽  
K. J. Sreeram ◽  
J. Raghava Rao ◽  
...  
Keyword(s):  
Industry Waste ◽  
Pulp Industry ◽  
Paper And Pulp ◽  
Paper And Pulp Industry

Industrial applications of lignin-transforming enzymes

1987 ◽  
Vol 321 (1561) ◽  
pp. 549-553 ◽  
Keyword(s):  
Paper Industry ◽  
White Rot Fungi ◽  
Industrial Applications ◽  
Culture Conditions ◽  
Extracellular Proteins ◽  
White Rot ◽  
White Rot Fungus ◽  
Efficient Manner ◽  
Isolation And Characterization ◽  
Specific Reactions

Lignin-degrading and modifying enzymes are produced under specific culture conditions by white-rot fungi. Most of these enzymes are excreted into the extracellular environment and can be purified from culture supernatant. At RepliGen we have characterized many of the extracellular proteins from the white-rot fungus Phanerochaete chrysosporium . Industrial application potentials for these enzymes are predicted to be in the chemical industry, pulp and paper industry, and perhaps in pollution control. The isolation and characterization of enzymes that catalyse specific reactions on kraft lignins and lignosulphonates will be discussed. These reactions include (1) a polymerizing—depolymerizing activity that changes the size of the lignin substrate and (2) a decolorizing reaction that reduces chromophoric groups in lignin. Kraft lignin and lignosulphonates have many diverse commercial applications because of their dual properties of hydrophobicity and hydrophilicity. They could have broad use if modified, as in the above reactions, in an efficient manner. The development of enzyme systems may provide just such efficient reactions.

Effect of Paper Sludge Ash on the Compressive Strength of Plaster of Paris/Paper Sludge Ash Composites

2012 ◽  
Vol 501 ◽  
pp. 34-38 ◽  
Author(s):  
Kar Keng Lim ◽  
Roslinda Shamsudin ◽  
Muhammad Azmi Abdul Hamid
Keyword(s):  
Compressive Strength ◽  
Construction Material ◽  
Paper Industry ◽  
Pulp And Paper Industry ◽  
Paper Sludge ◽  
Mixed Powder ◽  
Plaster Of Paris ◽  
Paper Sludge Ash ◽  
Sludge Ash ◽  
Cylindrical Samples

In this study, paper sludge ash, a waste from pulp and paper industry was used as a filler in fabricating Plaster of Paris/paper sludge ash composites. Various percentage of paper sludge ash was used, namely 1wt.%, 3wt.%, 5wt.% and 7wt.%. The effect of paper sludge ash on the compressive strength of the Plaster of Paris was studied. The mixed powder of paper sludge ash and Plaster of Paris were form into a 6 mm diameter and 12 mm height cylindrical samples. The composites were characterized theirs density where it shows that the density decreased as the amount of paper sludge ash increased. The compressive strength of the composites also decreased from 11.67 MPa without paper sludge ash addition to 0.50 MPa at 7wt.% paper sludge ash. However, the requirement of strength for Plaster of Paris in industry is between 8.96 MPa to 20.68 MPa. From the SEM observation, sample contain higher percentage of paper sludge ash exhibited more porosity. Therefore with the addition of 1wt.% of paper sludge ash into Plaster of Paris can be a promising construction material.

The effect of protic solvents in the conversion of lignin from Earleaf Acacia tree

2021 ◽  
Vol 11 (1) ◽  
pp. 123-126
Author(s):  
Khoa Phung Thanh ◽  
Khanh Vu Bao ◽  
Phat Huynh Van ◽  
Truc Nguyen Kim ◽  
An Tran Nguyen Minh ◽  
...  
Keyword(s):  
Aromatic Compounds ◽  
Solvent Polarity ◽  
Pulp Industry ◽  
Protic Solvents ◽  
Renewable Feedstock ◽  
Bulk Chemical ◽  
Paper And Pulp ◽  
Paper And Pulp Industry ◽  
Main Components ◽  
Acacia Tree

Lignin is one of main components of lignocellulosic along with cellulose and hemicellulose. It is a by-product of the paper and pulp industry, and has aromatic backbones making them an ideal renewable feedstock of aromatic compounds for a range of applications. Catalytic conversion of lignin from Earleaf Acacia tree was performed using high pressure/temperature reactor with Ru/C catalyst and protic solvents. The results showed that the conversion of lignin depends on the solvent polarity of protic solvents, and Ru/C catalyst enhanced the lignin conversion. Phenolic compounds are the main components of lignin conversion. Those compounds can be applied as a basement for bulk chemical and fuels.

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