scholarly journals Molecular Dissection for Boll Shedding in Upland Cotton Using Microsatellites

2021 ◽  
pp. 34-41
Author(s):  
Kanwal Zia ◽  
Syed Bilal Hussain
Keyword(s):  
Genetic Diversity ◽  
Dna Markers ◽  
Association Studies ◽  
Molecular Genetic ◽  
Phenotypic Traits ◽  
Marker Assisted Breeding ◽  
Molecular Genetic Diversity ◽  
Low Genetic Diversity ◽  
Cotton Genotypes ◽  
Single Cluster

DNA markers application in marker-assisted breeding of cotton is handicapped due to low genetic diversity in cotton germplasm. The present study was designed to identify DNA markers, predominately simple sequence repeats (SSRs), associated with tolerance/resistance to heat stress as a consequence of boll shedding. To find out the genetic diversity a total of 24 cotton genotypes and 50 SSR primers were used. Total 288 alleles were produced with an average of 5.7 alleles per primer. Bootstrap cluster analysis used to generate a dendrogram that cluster the 24 accessions into two main clusters. Eleven out of 24 genotypes fall in a single cluster. Phenotypically H-4074 gives more diversity, while genotypically H-4074 sheared the same genetic background as H-4070, H-4091 and H-4090. Low genetic diversity was observed among both genotypic and phenotypic as maximum varieties fall in single group. This study helps for selecting diverse accessions with multiple phenotypic traits, which were drought to boll shedding. It suggests further elaborating the molecular genetic diversity by using new SSR marker to improve the yield of cotton cultivars. These preliminary results set the stage for initiating in depth marker-trait association studies, which will be instrumental for initiating marker-assisted breeding in cotton.

Molecular genetic diversity of Punica granatum L. (pomegranate) as revealed by microsatellite DNA markers (SSR)

Gene ◽  
2012 ◽  
Vol 493 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Nejib Hasnaoui ◽  
Anna Buonamici ◽  
Federico Sebastiani ◽  
Messaoud Mars ◽  
Dapeng Zhang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Molecular Genetic ◽  
Punica Granatum ◽  
Molecular Genetic Diversity ◽  
Microsatellite Dna Markers ◽  
Punica Granatum L

Study on molecular genetic diversity of native duck breeds in China

2006 ◽  
Vol 62 (04) ◽  
pp. 603-611
Author(s):  
H. LI ◽  
N. YANG ◽  
K. CHEN ◽  
G. CHEN ◽  
Q. TANG ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Genetic ◽  
Molecular Genetic Diversity

Molecular characterisation of CGMS, maintainer and inbred lines and diversity analysis in pigeonpea [Cajanus cajan (L.) Millsp.]

2015 ◽  
Vol 38 (6) ◽  
Author(s):  
K. Indira Petchiammal ◽  
A. R. Muthiah ◽  
P. Jayamani
Keyword(s):  
Molecular Diversity ◽  
Molecular Genetic ◽  
Inbred Lines ◽  
Polymorphic Markers ◽  
Molecular Genetic Diversity ◽  
Ssr Primers ◽  
Parental Material ◽  
The Mean ◽  
Single Cluster ◽  
Different Sources

The present study was initiated with forty microsatellite markers for studying the molecular genetic diversity in 28 pigeonpea genotypes (6 CGMS lines of A<sub>1</sub>, A<sub>2</sub> and A<sub>4</sub> sources, their maintainer lines and 16 inbred lines) utilised as the parental material for the synthesis of F<sub>1</sub> hybrids. The level of polymorphism was too low and only 45 per cent markers were polymorphic. A total of 65 alleles were produced and the number of alleles produced ranged from two to six. The mean PIC value of 40 SSR primers was 0.147 and the primer CcM2977 produced the highest PIC value of 0.787 followed by CcM1770 (0.565). The primers <italic>viz.</italic>, CcM0039 and CcM2463 were polymorphic between CGMS and inbred lines and the primer CcM2977 was polymorphic among the different sources of CGMS lines. The UPGMA clustering and Neighbour-joining analyses grouped all the CGMS lines and respective maintainer lines in a single cluster and the inbred lines as two separate clusters. Among the inbred lines ICPL 11966, ICPL11947 and ICPL11950 were distinctly grouped in one cluster and other 13 inbred lines were clustered separately. Polymorphic markers <italic>viz.</italic>,CcM2977 and CcM1770 were used to confirm the hybrids of the crosses <italic>viz.</italic>, ICPA 2043 x LRG 41 and ICPA 2043 x IC 33725. The present study revealed that SSR markers are highly informative for exploiting the molecular diversity prevailing among the parental lines and could be utilized for further pigeonpea improvement at genomic level.

Study of molecular genetic diversity and evolutionary history of medicinally important endangered genus Chlorophytum using DNA barcodes

2016 ◽  
Vol 65 ◽  
pp. 245-252 ◽  
Author(s):  
Swapnil M. Patil ◽  
Niraj R. Rane ◽  
Avinash A. Adsul ◽  
Avinash R. Gholave ◽  
Shrirang R. Yadav ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Evolutionary History ◽  
Molecular Genetic ◽  
Dna Barcodes ◽  
Molecular Genetic Diversity ◽  
History Of
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