Developing the Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System

2021 ◽  
pp. 68-79
Author(s):  
Sina Mirzaahmadi ◽  
Golnaz Asaadi-Tehrani ◽  
Mojgan Bandehpour ◽  
Nooshin Davoudi ◽  
Leila Tahmasbi ◽  
...  
Keyword(s):  
Expression System ◽  
Coagulation Factor ◽  
Factor Vii ◽  
Coagulation Factor Vii ◽  
Human Coagulation Factor Vii

scholarly journals Expression of Recombinant Human Coagulation Factor VII by the LizardLeishmaniaExpression System

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Sina Mirzaahmadi ◽  
Golnaz Asaadi-Tehrani ◽  
Mojgan Bandehpour ◽  
Nooshin Davoudi ◽  
Leila Tahmasbi ◽  
...  
Keyword(s):  
Protein Expression ◽  
Western Blot ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Recombinant Protein Expression ◽  
Expression System ◽  
Coagulation Factor ◽  
Factor Vii ◽  
Coagulation Factor Vii ◽  
Human Coagulation Factor Vii

The variety of recombinant protein expression systems have been developed as a resource of FVII gene expression. In the current study, the authors used a novel protein expression system based on the Iranian LizardLeishmania, a trypanosomatid protozoan as a host for expression of FVII. Plasmid containing cDNA encoding full-length human FVII was introduced into LizardLeishmaniaand positive transfectants were analyzed by SDS-PAGE and Western blot analysis. Furthermore, biological activity of purified protein was detected by PT assay. The recombinant strain harboring a construct was analyzed for expression of FVII at the mRNA and protein level. Purified rFVII was obtained and in order to confirm the purified compound was in fact rFVII. Western blot analysis was carried out. Clotting time in PT assay was reduced about 30 seconds with the purified rFVII. In Conclusion, this study has demonstrated, for the first time, thatLeishmaniacells can be used as an expression system for producing recombinant FVII.

A Polymorphic Cluster in the 5′ Region of the Human Coagulation Factor VII Gene: Detection, Frequency, and Linkage Disequilibrium

1997 ◽  
Vol 88 (5) ◽  
pp. 445-448 ◽  
Author(s):  
Giorgio Dell'Acqua ◽  
Licia Iacoviello ◽  
Andria D'Orazio ◽  
Rosa Di Bitondo ◽  
Augusto Di Castelnuovo ◽  
...  
Keyword(s):  
Linkage Disequilibrium ◽  
Coagulation Factor ◽  
Factor Vii ◽  
Gene Detection ◽  
Coagulation Factor Vii ◽  
Detection Frequency ◽  
Human Coagulation Factor Vii

scholarly journals A frequent human coagulation Factor VII mutation (A294V, c152) in loop 140s affects the interaction with activators, tissue factor and substrates

2002 ◽  
Vol 363 (2) ◽  
pp. 411 ◽  
Author(s):  
Raffaella TOSO ◽  
Mirko PINOTTI ◽  
Katherine A. HIGH ◽  
Eleanor S. POLLAK ◽  
Francesco BERNARDI
Keyword(s):  
Tissue Factor ◽  
Coagulation Factor ◽  
Factor Vii ◽  
Coagulation Factor Vii ◽  
Human Coagulation Factor Vii

A missense mutation (178Cys↑Tyr) and two neutral dimorphisms (115His and 333Ser) in the human coagulation factor VII gene

1993 ◽  
Vol 2 (7) ◽  
pp. 1055-1056 ◽  
Author(s):  
G. Marchetti ◽  
M. Ferrati ◽  
P. Patracchini ◽  
R. Redaelli ◽  
F. Bernardi
Keyword(s):  
Missense Mutation ◽  
Coagulation Factor ◽  
Factor Vii ◽  
Coagulation Factor Vii ◽  
Human Coagulation Factor Vii
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