Review: Early Detection of Mycoplasma hyopneumoniae in Pigs Using Tracheo-bronchial Swab Sampling under Field Conditions

2021 ◽  
pp. 52-62
Author(s):  
F. Vangroenweghe
Keyword(s):  
Early Detection ◽  
Mycoplasma Hyopneumoniae ◽  
Field Conditions

Detection of Mycoplasma hyopneumoniae by Air Sampling with a Nested PCR Assay

1998 ◽  
Vol 64 (2) ◽  
pp. 543-548 ◽  
Author(s):  
Katharina D. C. Stärk ◽  
Jacques Nicolet ◽  
Joachim Frey
Keyword(s):  
Nested Pcr ◽  
Gene Segment ◽  
Mycoplasma Hyopneumoniae ◽  
Field Conditions ◽  
Pcr Analysis ◽  
Direct Extraction ◽  
Pcr Assay ◽  
Sampling System ◽  
Respiratory Problems ◽  
One Step

ABSTRACT This article describes the first successful detection of airborneMycoplasma hyopneumoniae under experimental and field conditions with a new nested PCR assay. Air was sampled with polyethersulfone membranes (pore size, 0.2 μm) mounted in filter holders. Filters were processed by dissolution and direct extraction of DNA for PCR analysis. For the PCR, two nested pairs of oligonucleotide primers were designed by using an M. hyopneumoniae-specific DNA sequence of a repeated gene segment. A nested PCR assay was developed and used to analyze samples collected in eight pig houses where respiratory problems had been common. Air was also sampled from a mycoplasma-free herd. The nested PCR was highly specific and 104 times as sensitive as a one-step PCR. Under field conditions, the sampling system was able to detect airborne M. hyopneumoniae on 80% of farms where acute respiratory disease was present. No airborne M. hyopneumoniae was detected on infected farms without acute cases. The chance of successful detection was increased if air was sampled at several locations within a room and at a lower air humidity.

The Mycoplasma hyopneumoniae recombinant heat shock protein P42 induces an immune response in pigs under field conditions

2014 ◽  
Vol 37 (4) ◽  
pp. 229-236 ◽  
Author(s):  
Sérgio Jorge ◽  
Natasha Rodrigues de Oliveira ◽  
Silvana Beutinger Marchioro ◽  
Andressa Fisch ◽  
Charles Klazer Gomes ◽  
...  
Keyword(s):  
Immune Response ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Mycoplasma Hyopneumoniae ◽  
Field Conditions

scholarly journals Evaluation of Mycoplasma hyopneumoniae Inactivated Vaccine in Pigs under Field Conditions.

1999 ◽  
Vol 61 (10) ◽  
pp. 1131-1135 ◽  
Author(s):  
Munenori OKADA ◽  
Tetsuya SAKANO ◽  
Kazuhiro SENNA ◽  
Takashi MARUYAMA ◽  
Junichi MUROFUSHI ◽  
...  
Keyword(s):  
Mycoplasma Hyopneumoniae ◽  
Field Conditions ◽  
Inactivated Vaccine

scholarly journals Mycoplasma hyopneumoniae vaccination at or shortly before weaning under field conditions: a randomised efficacy trial

2017 ◽  
Vol 181 (1) ◽  
pp. 19-19 ◽  
Author(s):  
I. Arsenakis ◽  
A. Michiels ◽  
R. Del Pozo Sacristán ◽  
F. Boyen ◽  
F. Haesebrouck ◽  
...  
Keyword(s):  
Mycoplasma Hyopneumoniae ◽  
Field Conditions ◽  
Efficacy Trial

scholarly journals Performance of Commercial Mycoplasma hyopneumoniae Serum Enzyme-Linked Immunosorbent Assays under Experimental and Field Conditions

2020 ◽  
Vol 58 (12) ◽  
Author(s):  
Ana Paula S. Poeta Silva ◽  
Ronaldo L. Magtoto ◽  
Henrique M. Souza Almeida ◽  
Aric McDaniel ◽  
Precy D. Magtoto ◽  
...  
Keyword(s):  
Serum Antibody ◽  
Mycoplasma Hyopneumoniae ◽  
Error Rates ◽  
False Positives ◽  
Field Conditions ◽  
Misclassification Error ◽  
Antibody Detection ◽  
Serum Samples ◽  
Content Type ◽  
Immunosorbent Assays

ABSTRACT Mycoplasma hyopneumoniae is an economically significant pathogen of swine. M. hyopneumoniae serum antibody detection via commercial enzyme-linked immunosorbent assays (ELISAs) is widely used for routine surveillance in commercial swine production systems. Samples from two studies were used to evaluate assay performance. In study 1, 6 commercial M. hyopneumoniae ELISAs were compared using serum samples from 8-week-old cesarean-derived, colostrum-deprived (CDCD) pigs allocated to the following 5 inoculation groups of 10 pigs each: (i) negative control, (ii) Mycoplasma flocculare (strain 27399), (iii) Mycoplasma hyorhinis (strain 38983), (iv) Mycoplasma hyosynoviae (strain 34428), and (v) M. hyopneumoniae (strain 232). Weekly serum and daily oral fluid samples were collected through 56 days postinoculation (dpi). The true status of pigs was established by PCR testing on oral fluids samples over the course of the observation period. Analysis of ELISA performance at various cutoffs found that the manufacturers’ recommended cutoffs were diagnostically specific, i.e., produced no false positives, with the exceptions of 2 ELISAs. An analysis based on overall misclassification error rates found that 4 ELISAs performed similarly, although one assay produced more false positives. In study 2, the 3 best-performing ELISAs from study 1 were compared using serum samples generated under field conditions. Ten 8-week-old pigs were intratracheally inoculated with M. hyopneumoniae. Matched serum and tracheal samples (to establish the true pig M. hyopneumoniae status) were collected at 7- to 14-day intervals through 98 dpi. Analyses of sensitivity and specificity showed similar performance among these 3 ELISAs. Overall, this study provides an assessment of the performance of current M. hyopneumoniae ELISAs and an understanding of their use in surveillance.

The effect of vaccination on the transmission of Mycoplasma hyopneumoniae in pigs under field conditions

2011 ◽  
Vol 188 (1) ◽  
pp. 48-52 ◽  
Author(s):  
I. Villarreal ◽  
T. Meyns ◽  
J. Dewulf ◽  
K. Vranckx ◽  
D. Calus ◽  
...  
Keyword(s):  
Mycoplasma Hyopneumoniae ◽  
Field Conditions

scholarly journals Early Detection of Maedi-Visna (Ovine Progressive Pneumonia) Virus Seroconversion in Field Sheep Samples

2001 ◽  
Vol 13 (4) ◽  
pp. 301-307 ◽  
Author(s):  
R. Varea ◽  
E. Monleón ◽  
C. Pacheco ◽  
L. Luján ◽  
R. Bolea ◽  
...  
Keyword(s):  
Early Detection ◽  
False Negative ◽  
Field Conditions ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Negative Results ◽  
Visna Virus ◽  
False Negative Results ◽  
Ovine Progressive Pneumonia ◽  
Antibody Levels

The aim of this work was to investigate whether an enzyme-linked immunosorbent assay (ELISA) was useful for early detection of maedi-visna virus (MVV) infection in sheep under field conditions. An ELISA based on p25 recombinant protein and a gp46 synthetic peptide was used. Sequentially obtained serum samples ( n = 1,941) were studied for 4 years. ELISA results were compared with those of the agar gel immunodiffusion (AGID) test, and results of both tests were compared with a reference result established using consensus scores for at least 2 of 3 serologic techniques (AGID, ELISA, and western blotting, which was used to resolve result discrepancies between the other 2 techniques). A total of 247 discrepancies were observed between ELISA and AGID. Of these, 131 were due to an earlier detection of 120 sera by the ELISA and 11 sera by AGID. The remaining discrepancies (116) were due to the presence of false reactions in both tests. Fewer false-negative results were found by ELISA than with AGID (6 vs. 69 sera, respectively), whereas the number of false-positive results was virtually the same for ELISA and AGID (21 vs. 20, respectively). In relation to the reference result, ELISA sensitivity and specificity were 97.8% and 98.2%, respectively, whereas values for AGID were 76.3% and 98.3%, respectively. The agreement between ELISA and the reference result was higher than that between AGID and the reference result (κ value: 0.96 and 0.77, respectively). A variation in the ELISA signal (based on optical density) was observed during the study period, suggesting different antibody levels throughout the animal's life. The ELISA was useful for detecting MVV-infected sheep in field conditions and has potential for use in control and eradication programs.

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