scholarly journals Differentiation and Characterization of Mycobacterium tuberculosis Complex Causing Pulmonary Tuberculosis across North Central Nigeria

2019 ◽  
pp. 1-10
Author(s):  
Aleruchi Chuku ◽  
Bukola Ajide ◽  
Lovett Lawson ◽  
Kayode Orule ◽  
Lillian Adogo
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Central Zone ◽  
Tuberculosis Complex ◽  
Central State ◽  
General Hospitals ◽  
North Central ◽  
The North ◽  
Suspected Tuberculosis

Aims: To differentiate and Characterize Mycobacterium tuberculosis complex causing pulmonary tuberculosis across North Central Nigeria Study Design: This was a simple descriptive health-based study that involved clinically suspected tuberculosis patients who were referred to two selected General Hospitals for diagnosis in each of the states across North Central Nigeria. Place and Duration of Study: This study was carried out in the North Central zone of Nigeria and it included all the seven States across North Central Nigeria using two General Hospitals per state. The study included 371 positive sputum samples drawn from 2800 suspected pulmonary TB patients between 2017 and 2018. Methodology: A total of 371 GeneXpert positive sputa for TB were decontaminated using N Acetyl Cysteine and Sodium Hydroxide under a level 3 Bio-safety cabinet and the resulting sediment was cultured on Lowestein- Jensen (LJ) media containing glycerol and pyruvate at 37ºC in a slanted position, SD- Bioline (TB Ag MPT 64) for the differentiation of MTBC from NTM was carried out using the isolates from LJ culture. Evaluation of speciation was done using Line Probe Assay to determine the predominant species of MTBC. All the protocols used in this study followed the manufacturer’s manual strictly. Results: A total of 371 decontaminated positive GeneXpert sputa derived from 2800 suspected PTB participants were cultured on Lowestein- Jensen (LJ) medium and 302(81.40%) was found positive while 69(18.60%) were found negative. Out of the culture positive isolates, 288 (95.36%) isolates were detected on SD-BIOLINE TB Ag MPT 64 ® assay for MTBC and 14 (4.64%) as NTM. Of the 288 MTBC, three different species were identified; 272 (94.64%) were M. tuberculosis/M. Canetti, 7 (2.43%) were M. africanum and 9(3.13) showed a no MTBC reaction band on all the samples that were analysed. Conclusion: Differentiations of MTBC from NTM has help to re-confirm that not all symptoms of pulmonary infection are caused by MTBC but NTM are implicated due to their distribution in the environment, however, molecular characterisation method has narrow our findings down to M. tuberculosis/M. canettii as the predominant specie of MTBC circulating in the region, although, M. africaum was also detected and these two species of MTBC are the leading cause of pulmonary tuberculosis across all the North Central state of Nigeria.

scholarly journals 1633. Improving the diagnosis of extra-pulmonary tuberculosis (EPTB): experience from north India

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S808-S808
Author(s):  
Anchal Sharma ◽  
Kusum Sharma ◽  
Manish Modi ◽  
Aman Sharma
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Lamp Assay ◽  
Nucleic Acid Amplification ◽  
Rural Setting ◽  
Tuberculosis Complex ◽  
Extra Pulmonary Tuberculosis ◽  
Resource Poor ◽  
Extra Pulmonary

Abstract Background Rapid and accurate diagnosis of extra-pulmonary tuberculosis (EPTB) is imperative for early treatment and better patient outcome. Loop-mediated Isothermal Amplification (LAMP) is a promising nucleic-acid amplification assay. LAMP assay could be carried out in simple water bath under isothermal conditions in 60 minutes, and can be performed in any laboratory even in rural setting in resource poor endemic countries. We evaluated LAMP assay using two different target regions LAMP primers specific for Mycobacterium tuberculosis complex for the diagnosis of EPTB. Methods LAMP assay using 6 primers (each for IS6110 and IS1081) specific for Mycobacterium tuberculosis complex were performed on patients suspected of EPTB on various EPTB samples(CSF, Synovial fluid, Lymaphnode and tissue biopsies and various other samples) of 150 patients (50 confirmed, 100 suspected) Clinically suspected of EPTB and 100 non-TB control subjects. Results Overall LAMP test (using any of the two targets) had sensitivity and specificity of 96% and 100% for confirmed (50 culture positive) EPTB cases. In 100 clinically suspected but unconfirmed EPTB cases, LAMP was positive in 87 out of 100 cases (87%). Sensitivity of IS6110 LAMP, 1S1081 LAMP and IS6110 PCR for clinically suspected cases was 78 (78%), 84 (84%) and 70 (70%), respectively. In total 150 EPTB patients, the overall sensitivity of microscopy, culture, IS6110 PCR, IS6110 LAMP, 1081 LAMP and the LAMP test (if any of the two targets were used) were 4%, 33.3%, 74.6%, 82.66%, 87% and 92%, respectively. Specificity of all the tests was 100%. There were 8 cases which were missed by IS6110 LAMP and 2 cases by 1081 LAMP. Conclusion LAMP assay using two targets is a promising technique for rapid diagnosis of EPTB in 60 minutes especially in a resource poor setting who are still battling with this deadly disease. Disclosures All Authors: No reported disclosures

scholarly journals Molecular Analysis of Mycobacterium tuberculosis Isolated in the North Central Zone of Nigeria

2019 ◽  
Vol 9 (4) ◽  
pp. 259 ◽  
Author(s):  
Benjamin David Thumamo Pokam ◽  
Dorothy Yeboah-Manu ◽  
Lovett Lawson ◽  
Prisca Wabo Guemdjom ◽  
Ruth Okonu ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Molecular Analysis ◽  
Central Zone ◽  
North Central ◽  
The North

scholarly journals Mycobacterium Tuberculosis and Nontuberculous Mycobacteria Isolates from Presumptive Pulmonary Tuberculosis Patients Attending A Tertiary Hospital in Addis Ababa, Ethiopia

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Daniel Kahase ◽  
Kassu Desta ◽  
Zelalem Yaregal ◽  
Bazezew Yenew ◽  
Getu Driba ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Nontuberculous Mycobacteria ◽  
Addis Ababa ◽  
Public Health Problem ◽  
Sub Saharan Africa ◽  
Large Sample Size ◽  
Tuberculosis Complex ◽  
Tuberculosis Patients

BACKGROUND፡ Mycobacterial infections are known to cause a public health problem globally. The burden of pulmonary disease from nontuberculous mycobacteria is reportedly on the rise in different parts of the world despite the fact that there is limited data about the disease in sub-Saharan Africa including Ethiopia. Hence, we aimed to assess the magnitude of M. tuberculosis and nontuberculous mycobacteria (NTM) among presumptive pulmonary tuberculosis patients attending St. Paul’s hospital Medical College, Addis Ababa, Ethiopia.METHODS: A cross-sectional study was conducted from June to September 20/2016. Morning sputum specimens were collected, processed and cultured in Lowenstein Jensen medium and BACTEC MGIT 960 media. The nontuberculous mycobacteria were further confirmed and characterized by Genotype CM/AS assays. The socio-demographic, clinical and chest x-ray data were collected using a structured questionnaire. The data was analyzed using SPSS version 20.RESULTS: Out of 275 presumptive tuberculosis patients enrolled in the study, 29(10.5%) were culture positive for Mycobacteria. Of these, 3(10.3%) were found to be NTM and 26(89.6%) were Mycobacterium tuberculosis complex. Of the NTM, two were unidentified and one typed as M.peregrinum. There was no coisolation of Mycobacterium tuberculosis complex and nontuberculous mycobacteria. Overall, 6(23.1%) Mycobacterium tuberculosis complex isolates were resistant to at least one antituberculosis drug. Of these, two were multidrug resistanttuberculosis cases (7.7%) detected from previously treated patients.CONCLUSION: Relatively low magnitude of Mycobacterium tuberculosis complex and nontuberculous mycobacteria isolates were seen in the study area. Therefore, further study using a large sample size is needed to be done to consider nontuberculous mycobacteria infection as a differential diagnosis in presumptive pulmonary tuberculosis patients.

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