Molecular Detection of Mycobacterium Tuberculosis Complex from Sputum of Clinically Suspected Tuberculosis Cases

2017 ◽  
Vol 3 (2) ◽  
Author(s):  
Neha Dayal ◽  
Umar Farooq ◽  
Mazher Maqusood ◽  
Sana Nudrat ◽  
Bhavna Bhadauria ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Molecular Detection ◽  
Mycobacterium Tuberculosis Complex ◽  
Tuberculosis Complex ◽  
Suspected Tuberculosis

Molecular detection of Mycobacterium tuberculosis complex in the 8th century skeletal remains from the territory of Slovakia

Biologia ◽  
2016 ◽  
Vol 71 (6) ◽  
Author(s):  
Klaudia Kyselicová ◽  
Lukáš Šebest ◽  
Csaba Bognár ◽  
Michal Šarkan ◽  
Marián Baldovič ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Young Adult ◽  
Adult Male ◽  
Molecular Detection ◽  
Mycobacterium Tuberculosis Complex ◽  
Archaeological Site ◽  
Skeletal Remains ◽  
Tuberculosis Complex ◽  
Young Adult Male ◽  
Silica Bead

AbstractDNA was extracted using a Silica Bead Extraction kit from bone samples taken from a Slavonic-Avar individual found at the archaeological site of Cífer-Pác. The analysed skeletal remains from the grave number 62/79 belong to a young adult male (20–30 years at death) and are dated to the 8

scholarly journals Widespread Environmental Contamination with Mycobacterium tuberculosis Complex Revealed by a Molecular Detection Protocol

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142079 ◽  
Author(s):  
Nuno Santos ◽  
Catarina Santos ◽  
Teresa Valente ◽  
Christian Gortázar ◽  
Virgílio Almeida ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Environmental Contamination ◽  
Molecular Detection ◽  
Mycobacterium Tuberculosis Complex ◽  
Tuberculosis Complex

scholarly journals Differentiation and Characterization of Mycobacterium tuberculosis Complex Causing Pulmonary Tuberculosis across North Central Nigeria

2019 ◽  
pp. 1-10
Author(s):  
Aleruchi Chuku ◽  
Bukola Ajide ◽  
Lovett Lawson ◽  
Kayode Orule ◽  
Lillian Adogo
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Central Zone ◽  
Tuberculosis Complex ◽  
Central State ◽  
General Hospitals ◽  
North Central ◽  
The North ◽  
Suspected Tuberculosis

Aims: To differentiate and Characterize Mycobacterium tuberculosis complex causing pulmonary tuberculosis across North Central Nigeria Study Design: This was a simple descriptive health-based study that involved clinically suspected tuberculosis patients who were referred to two selected General Hospitals for diagnosis in each of the states across North Central Nigeria. Place and Duration of Study: This study was carried out in the North Central zone of Nigeria and it included all the seven States across North Central Nigeria using two General Hospitals per state. The study included 371 positive sputum samples drawn from 2800 suspected pulmonary TB patients between 2017 and 2018. Methodology: A total of 371 GeneXpert positive sputa for TB were decontaminated using N Acetyl Cysteine and Sodium Hydroxide under a level 3 Bio-safety cabinet and the resulting sediment was cultured on Lowestein- Jensen (LJ) media containing glycerol and pyruvate at 37ºC in a slanted position, SD- Bioline (TB Ag MPT 64) for the differentiation of MTBC from NTM was carried out using the isolates from LJ culture. Evaluation of speciation was done using Line Probe Assay to determine the predominant species of MTBC. All the protocols used in this study followed the manufacturer’s manual strictly. Results: A total of 371 decontaminated positive GeneXpert sputa derived from 2800 suspected PTB participants were cultured on Lowestein- Jensen (LJ) medium and 302(81.40%) was found positive while 69(18.60%) were found negative. Out of the culture positive isolates, 288 (95.36%) isolates were detected on SD-BIOLINE TB Ag MPT 64 ® assay for MTBC and 14 (4.64%) as NTM. Of the 288 MTBC, three different species were identified; 272 (94.64%) were M. tuberculosis/M. Canetti, 7 (2.43%) were M. africanum and 9(3.13) showed a no MTBC reaction band on all the samples that were analysed. Conclusion: Differentiations of MTBC from NTM has help to re-confirm that not all symptoms of pulmonary infection are caused by MTBC but NTM are implicated due to their distribution in the environment, however, molecular characterisation method has narrow our findings down to M. tuberculosis/M. canettii as the predominant specie of MTBC circulating in the region, although, M. africaum was also detected and these two species of MTBC are the leading cause of pulmonary tuberculosis across all the North Central state of Nigeria.

scholarly journals Five Years’ Evaluation of the BD ProbeTec System for the Direct Molecular Detection of Mycobacterium tuberculosis Complex in Respiratory and Nonrespiratory Clinical Samples

2015 ◽  
Vol 64 (4) ◽  
pp. 391-394 ◽  
Author(s):  
Can Bicmen ◽  
Onur Karaman ◽  
Ayriz T. Gunduz ◽  
Onur F. Erer ◽  
Meral Coskun ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Early Diagnosis ◽  
Molecular Detection ◽  
Mycobacterium Tuberculosis Complex ◽  
Clinical Samples ◽  
Tuberculosis Complex ◽  
Predictive Values ◽  
Smear Negative ◽  
Sensitivity Specificity ◽  
Culture Negative

In this study, Mycobacterium tuberculosis complex was detected by BD ProbeTec ET system in 4716 respiratory and 167 nonrespiratory samples [mostly (98%) smear negative]. Sensitivity, specificity, positive and negative predictive values were 81.8%, 98.3, 85.1 and 97.9 for respiratory and 100%, 96.2, 64.7 and 100, for nonrespiratory samples, respectively. Among 149 (3.1%) ProbeTec DTB positive and culture negative samples, 72 (65 respiratory and seven nonrespiratory) (48.3%) were recovered from the patients who were evaluated as having TB infection. The system has been found as useful in early diagnosis of tuberculosis infection in association with the clinical, radiological and histopathological findings.

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