Comparison between Immunochromatographic Test and Enzyme Linked Immunosorbent Assay in Diagnosis of Helicobacter pylori Infection among Gastritis Patients in Khartoum State-Sudan

Background: The gold standard for the diagnosis of Helicobacter pylori infection requires an endoscopic biopsy of gastric mucosa for histological examination, urease test and culture; however serological tests are useful as a screening test for Helicobacter pylori infection. Objective: To compare between Immunochromatographic Test and Enzyme Linked Immunosorbent Assay techniques in detection of Helicobacter pylori immunoglobulin gamma antibodies in serum of patients suffer from gastritis. Materials and Methods: 245 patients were screened for Helicobacter pylori infections by rapid urease test. Sera from these patients were tested for anti- Helicobacter pylori immunoglobulin gamma antibodies by Enzyme Linked Immunosorbent Assay and Immunochromatographic Test techniques. Results: Of 245 patients tested, Immunochromatographic Test positive/negative 114 (46.5%)/131 (53.5%), whereas Enzyme Linked Immunosorbent Assay positive/negative were 124 (50.6%)/121 (49.4%). Sensitivity/ specificity was 67.4%/74.5% and 90.2%/89.3% for Immunochromatographic Test/Enzyme Linked Immunosorbent Assay positive/negative, respectively. The diagnostic accuracy was 71%/89.7% for Immunochromatographic Test/Enzyme Linked Immunosorbent Assay, respectively. Conclusion: The Enzyme Linked Immunosorbent Assay technique was found to be more sensitive, specific and accurate compared to the Immunochromatographic Test while The Immunochromatographic Test is commercially available, inexpensive and easy to perform compared to the Enzyme Linked Immunosorbent Assay.

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Association of gastric infection and periodontal disease through Helicobacter pylori as a common denominator: A systematic review and meta-analysi

10.37766/inplasy2021.10.0097 ◽

2021 ◽

Author(s):

Nansi López-Valverde ◽

◽

Antonio López-Valverde ◽

Ana Suarez ◽

Bruno Macedo de Sousa ◽

...

Keyword(s):

Systematic Review ◽

Helicobacter Pylori ◽

Clinical Studies ◽

Periodontal Diseases ◽

Meta Analysis ◽

Helicobacter Pylori Infection ◽

Enzyme Linked Immunosorbent Assay ◽

Rapid Urease Test ◽

Common Denominator ◽

Urease Test

Review question / Objective: Is gastric helicobacter pylori infection related to periodontal diseases? Condition being studied: Therefore, the aim of this systematic review and meta-analysis was to identify and analyze clinical studies to determine the direct correlation between Helicobacter Pylori gastric infection andPeriodontal Disease. Study designs to be included: Clinical studies that provided data on Helicobacter Pylori infection in both the stomach and oral cavity, confirmed by polymerase chain reaction (PCR), rapid urease test (RUT) or enzyme-linked immunosorbent assay (ELISA). Clinical studies that associated PD with Helicobacter Pylori. The diagnosis of PD was confirmed ac-cording to the diagnostic criteria in periodontology.

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Helicobacter pylori infection in diabetes mellitus patients with peptic ulcer disease

IMC Journal of Medical Science ◽

10.3329/imcjms.v14i2.52832 ◽

2021 ◽

Vol 14 (2) ◽

pp. 27-32

Author(s):

Salma Khatun ◽

Khandaker Shadia ◽

Mafruha Mahmud ◽

Sraboni Mazumder ◽

Indrajit Kumar Dutta ◽

...

Keyword(s):

Diabetes Mellitus ◽

Helicobacter Pylori ◽

Peptic Ulcer ◽

Helicobacter Pylori Infection ◽

Enzyme Linked Immunosorbent Assay ◽

Diabetic Patients ◽

Rapid Urease Test ◽

Urease Test ◽

Stool Antigen ◽

H Pylori

Background and objectives: Helicobacter pylori infection is suspected to be associated with extra-gastrointestinal disorders such as diabetes mellitus (DM). It is still a subject of investigation whether H. pylori has a pathogenic role on DM or diabetic patients have an increased susceptibility to H. pylori infection. The aim of the present study was to find out the rate of H. pylori infection in individuals with and without DM. Materials and methods: The study was conducted on 72 diabetic and 19 non-diabetic adult individuals with dyspeptic symptoms attending the BIRDEM General Hospital for diagnostic endoscopy. All cases were tested for H. pylori stool antigen by rapid immunochromatographic test (ICT), urease production in biopsy samples by rapid urease test (RUT), and serum anti-H. pylori IgA and anti-CagA IgG antibodies by enzyme-linked immunosorbent assay (ELISA). Any case that had peptic ulcer/erosion and was positive for H. pylori stool antigen or rapid urease test (RUT) was defined as H. pylori positive case. Results: There was no significant (p=0.095) difference in H. pylori infection between diabetics and non-diabetics (68.1% vs 47.4%). Presence of ulcer and erosion were not significantly different among diabetics and non-diabetics. Anti-H. pylori IgA positivity rate in H. pylori positive diabetic and non-diabetic cases were 65.3% and 55.6% (p=0.575) respectively while anti-CagA IgG rate in those cases were 46.9% and 66.7% (p=0.276) respectively. Conclusion: The present study did not reveal any significant difference in H. pylori infection between individuals with and without DM having peptic ulcer/erosion. Ibrahim Med. Coll. J. 2020; 14(2): 27-32

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Helicobacter pylori Infection and Strain Types in Adult Dyspeptic Patients Undergoing Endoscopy in a Specialized Hospital of Dhaka City

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v3i1.2963 ◽

2009 ◽

Vol 3 (1) ◽

pp. 4-9

Author(s):

Sufi HZ Rahman ◽

M Anisur Rahman ◽

MS Arfin ◽

M Mahbub Alam ◽

TM Bhuiyan ◽

...

Keyword(s):

Helicobacter Pylori ◽

Western Blot ◽

Blot Analysis ◽

Western Blot Analysis ◽

Helicobacter Pylori Infection ◽

Enzyme Linked Immunosorbent Assay ◽

Rapid Urease Test ◽

Type I ◽

Urease Test ◽

H Pylori

Helicobacter pylori infection occurs worldwide with a high prevalence in developing countries. Virulence of H. pylori strains varies in different geographic regions. The aim of this study was to see H. pylori infection and its strain types in adult dyspeptic patients in Bangladesh and to analyze association of H. pylori strain types with clinical disease and severity of histological gastritis. Ninety consecutive adult dyspeptic patients undergoing diagnostic endoscopy were tested for H. pylori infection by culture, rapid urease test (RUT), histology and anti H. pylori IgG ELISA (Enzyme linked immunosorbent assay). H. pylori strain types were determined by Western Blot analysis. Association of strain types with clinical gastro-duodenal diseases and grades of histological gastritis were analyzed by χ2 test. Among the selected patients, 53 (58.9%) were culture positive, 48 (53.3%) were RUT positive, 31 (34.4%) were histology positive and 82 (91.1%) were anti-H. pylori IgG ELISA positive. By Western Blot analysis of the 90 sera samples, 48 (53.3%) showed antibodies to Type I strain of H. pylori, 21 (23.3%) Intermediate strain and 3 (3.3%) Type II strain. Endoscopically, 20 (22.2%) patients were found normal, 27 (30.0%) had gastritis, 9 (10.0%) had duodenitis, 28 (31.1%) had peptic ulcer disease, 4 (4.4%) had gastric carcinoma, and 2 (2.2%) had reflux esophagitis. Histologically, 34.4% had H. pylori, 44.4% had polymorhonuclear neutrophil (PMN), 100% had mononuclear cell (MNC) infiltration of different grades, 1.1% had atrophic gastritis and 2.2% had intestinal metaplasia of moderate grade. H. pylori strain types was not associated with clinical gastro-duodenal diseases or grades of PMN or MNC infiltration (p > 0.05) in these patients. Key words: Helicobacter pylori infection, H. pylori strain types, gastro-duodenal diseases, grades of gastritis doi: 10.3329/bjmm.v3i1.2963 Bangladesh J Med Microbiol 2009; 03 (01): 4-9

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