scholarly journals Evaluation of Simultaneous Saccharification and Fermentation of Oil Palm Empty Fruit Bunches for Xylitol Production

Author(s):  
Khairul Hadi Burhan ◽  
Made Tri Ari Penia Kresnowati ◽  
Tjandra Setiadi

The biological process route of xylitol production from lignocellulosic materials, via enzymatic hydrolysis which is followed by fermentation, offers a more sustainable or greener process than the chemical process route. Both the enzymatic hydrolysis and the fermentation processes are conducted at moderate process condition and thus require less energy and chemicals. However, the process proceeds slower than the chemical one. In order to improve process performance, the enzymatic hydrolysis and the fermentation processes can be integrated as Simultaneous Saccharification and Fermentation (SSF) configuration. This paper discusses the evaluation of SSF configuration on xylitol production from Oil Palm Empty Fruit Bunches (OPEFB). To integrate two processes which have different optimum temperature, the performance of each process at various temperature was first evaluated. Later, SSF was evaluated at various hydrolysis and fermentation time at each optimum temperature. SSF showed better process performance than the separated hydrolysis and fermentation processes. The best result was obtained from configuration with 72 hours of prior hydrolysis followed by simultaneous hydrolysis and fermentation, giving yield of 0.08 g-xylitol/g-OPEFB. Copyright © 2019 BCREC Group. All rights reserved 

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Vera BARLIANTI ◽  
Deliana DAHNUM ◽  
. MURYANTO ◽  
Eka TRIWAHYUNI ◽  
Yosi ARISTIAWAN ◽  
...  

Abstrak Sebagai salah satu Negara penghasil minyak kelapa sawit mentah (CPO), Indonesia juga menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah besar. TKKS terdiri dari-tiga-komponen utama, yaitu selulosa, hemiselulosa, dan lignin. Pengolahan awal TKKS secara alkalindi ikuti dengan hidrolisis TKKS secara enzimatik menggunakan kombinasi enzim selulase dan β-glukosidase akan menghasilkan gula-gula yang mudah difermentasi.  Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi substrat, kon-sentrasi enzim, dan suhu selama proses hidrolisis berlangsung.  Hasil yang diperoleh menunjukkan bahwa konsentrasi gula maksimum (194,78 g/L) dicapai pada konsentrasi TKKS 20% (b/v), konsentrasi campuran enzim yang terdiri dari selulase dan β-1,4 glukosidase sebesar 3,85% (v/v), dan suhu 50oC. Perbandingan antara selulase dan β-1,4 glukosidase adalah 5:1 dengan masing-masing aktivitas enzim sebesar 144.5 FPU/mL dan 63 FPU/mL. Hasil penelitian juga menunjukkan bahwa model kinetika yang sesuai untuk proses hidrolisis TKKS secara enzimatik adalah model kinetika Shen dan Agblevor dengan reakside aktivasi enzim orde satu.  Hasil ini mendukung studi kelayakan ekonomi dalam pemanfaatan TKKS untuk produksi bioetanol.AbstractAs one of the crude palm oil producers, Indonesia also produces empty fruit bunches (EFB)in large quantities. The oil palm EFB consist of cellulose, hemicellulose and lignin. Alkaline pretreatment of EFB, followed by enzymatic hydro-lysis of cellulose using combination of cellulase and β-glucosidase enzymes produce fermentable sugars. This paper reported the effects of substrate loading, enzyme concentration, and temperature of hydrolysis process on reducing sugar production. The  maximum  sugar  concentration (194.78 g/L) was produced at 50oC using 20% (w/v) EFB and 3.85% (v/v) mixed enzymes of cellulase and β-1,4 glucosidase in volume ratio of 5:1 (v/v), with enzyme activity of 144.5 FPU/mL and 63 FPU/mL, respectively. The results also showed that the suitable kinetic model for enzymatic hydrolysis process of oil palm EFB follow Shen and Agblevor model with first order of enzyme deactivation. These results support the economic feasibility study in utilization of EFB of oil palm for bioethanol production.    


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