Wet oxidation pretreatment, enzymatic hydrolysis and simultaneous saccharification and fermentation of clover–ryegrass mixtures

2008 ◽  
Vol 99 (18) ◽  
pp. 8777-8782 ◽  
Author(s):  
Carlos Martín ◽  
Mette H. Thomsen ◽  
Henrik Hauggaard-Nielsen ◽  
Anne BelindaThomsen
Keyword(s):  
Enzymatic Hydrolysis ◽  
Simultaneous Saccharification And Fermentation ◽  
Wet Oxidation ◽  
Simultaneous Saccharification

Fuel Ethanol Production from Wet Oxidised Corn Stover by S. Cerevisiae

2011 ◽  
Vol 343-344 ◽  
pp. 963-967 ◽  
Author(s):  
Zhang Qiang ◽  
Anne Belinda Thomsen
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Liquid Fraction ◽  
Raw Material ◽  
Wet Oxidation ◽  
Inhibition Effect ◽  
Simultaneous Saccharification

In order to find out appropriate process for ethanol production from corn stover, wet oxidation（195°C，15 minutes）and simultaneous saccharification and fermentation (SSF) was carried out to produce ethanol. The results showed that the cellulose recovery of 92.9% and the hemicellulose recovery of 74.6% were obtained after pretreatment. 86.5% of cellulose was remained in the solid cake . After 24h hydrolysis at 50°C using cellulase（Cellubrix L）,the achieved conversion of cellulose to glucose was 64.8%. Ethanol production was evaluated from dried solid cake and the hydrolysate was employed as liquid fraction . After 142 h of SSF with substrate concentration of 8% (W/V), ethanol yield of 73.1 % of the theoretical based on glucose in the raw material was obtained by S. cerevisiae(ordinary baker’ yeast) . The corresponding ethanol concentration and volumetric productivity were 17.2g/L and 0.121g/L.h respectively. The estimated total ethanol production was 257.7 kg/ton raw material by assuming consumption of both C-6 and C-5. No obvious inhibition effect occurred during SSF. These instructions give you the basic guidelines for preparing papers for WCICA/IEEE conference proceedings.

scholarly journals Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust

BioResources ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. 7474-7491
Author(s):  
Carolina Mónica Mendieta ◽  
Fernando Esteban Felissia ◽  
Ana María Arismendy ◽  
Julia Kruyeniski ◽  
María Cristina Area
Keyword(s):  
Enzymatic Hydrolysis ◽  
Simultaneous Saccharification And Fermentation ◽  
Design Tool ◽  
Cellulolytic Enzymes ◽  
Chemical Compositions ◽  
Pine Sawdust ◽  
Second Generation Bioethanol ◽  
Separate Hydrolysis And Fermentation ◽  
Experimental Values ◽  
Simultaneous Saccharification

This work aims to evaluate second-generation bioethanol production from the soda-ethanol pulp of pine sawdust via two strategies: separate hydrolysis and fermentation and simultaneous saccharification and fermentation. A kinetics study of the enzymatic hydrolysis of separate hydrolysis and fermentation was included as a design tool. Three soda-ethanol pulps (with different chemical compositions), Cellic® Ctec2 cellulolytic enzymes, and Saccharomyces cerevisiae IMR 1181 (SC 1181) yeast were employed. The obtained kinetic parameters were as follows: an apparent constant (k) of 11.4 h-1, which represents the link frequency between cellulose and cellulase; a Michaelis-Menten apparent constant (KM) of 23.5 gL-1, that indicates the cellulose/cellulase affinity; and the apparent constant of inhibition between cellulose-glucose and cellulase (KI), which was 2.9 gL-1, 3.1 gL-1, and 6.6 gL-1 for pulps 1, 2, and 3, respectively. The kinetic model was applicable, since the calculated glucose values fit the experimental values. High bioethanol yields were obtained for pulp 3 in the separate hydrolysis and fermentation and simultaneous saccharification and fermentation processes (89.3% and 100% after 13 h and 72 h, respectively).

Optimization of enzymatic hydrolysis for ethanol production by simultaneous saccharification and fermentation of wastepaper

2011 ◽  
Vol 29 (11) ◽  
pp. 1134-1144 ◽  
Author(s):  
Kanokphorn Sangkharak
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ethanol Production ◽  
Incubation Time ◽  
Simultaneous Saccharification And Fermentation ◽  
Trichoderma Viride ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Sugar Production ◽  
Simultaneous Saccharification ◽  
Office Paper

The present study investigated the development of high sugar production by optimization of an enzymatic hydrolysis process using both conventional and statistical methods, as well as the production of ethanol by the selected wastepaper source. Among four sources of pretreated wastepaper including office paper, newspaper, handbills and cardboard, office paper gave the highest values of cellulose (87.12%) and holocelluloses (89.07%). The effects of the amount of wastepaper, the pretreatment method and the type of enzyme on reducing sugar production from office paper were studied using conventional methods. The highest reducing sugar production (1851.28 µg L−1; 37.03% conversion of glucose) was obtained from the optimal condition containing 40 mg of office paper, pretreated with stream explosion and hydrolysed with the combination of cellulase from Aspergillus niger and Trichoderma viride at the fixed loading rate of 20 FPU g−1 sample. The effects of interaction of wastepaper amount and enzyme concentration as well as incubation time were studied by a statistical method using central composite design. The optimal medium composition consisted of 43.97 µg L−1, 28.14 FPU g−1 sample and 53.73 h of wastepaper, enzyme concentration and incubation time, respectively, and gave the highest amount of sugar production (2184.22 µg L−1) and percentage conversion of glucose (43.68%). The ethanol production from pretreated office paper using Saccharomyces cerevisiae in a simultaneous saccharification and fermentation process was 21.02 g L−1 after 36 h of cultivation, corresponding to an ethanol volumetric production rate of 0.58 g ethanol L−1 h−1.

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