Determination of the Activity of Alpha Naphthyl Acetate Esterase and Acid Phosphatase of Peripheral Blood Lymphocytes in Healty Pregnant Merino Sheep

This study was aimed to determine the effects of the glucomannan added to aflatoxin- (AF-) contaminated diet on the sacculus rotundus and peripheral blood lymphocytes of New Zealand rabbits by histological and enzyme histochemical methods. Twenty-four adult rabbits of both sexes were divided into four equal groups, namely, as control, glucomannan 0.2 g/day, AF 125 μg/kg/day, and glucomannan combined with AF. The animals in all groups were treated for 12 weeks by the above-mentioned diet. When compared to control, AF-treatment caused significant decrease in alpha-naphthyl acetate esterase- (ANAE-) positive peripheral blood lymphocyte (PBL) percentages. The addition of the glucomannan to AFcontaining diet recovered the adverse effects of AF on sacculus rotundus and increased the ANAE-positive PBL counts. These results suggested that glucomannan was effective against the negative effects of AF in rabbits.

Download Full-text

Factors Affecting Acidα-Naphthyl Acetate Esterase Activity in Peripheral Blood Lymphocytes

American Journal of Clinical Pathology ◽

10.1093/ajcp/77.5.611 ◽

1982 ◽

Vol 77 (5) ◽

pp. 611-614 ◽

Cited By ~ 5

Author(s):

Jules M. Elias ◽

Frederick Miller

Keyword(s):

Peripheral Blood ◽

Esterase Activity ◽

Peripheral Blood Lymphocytes ◽

Factors Affecting ◽

Blood Lymphocytes ◽

Acetate Esterase ◽

Naphthyl Acetate

Download Full-text

Lipid Peroxidation, Antioxidant Level, and Alpha Naphthyl Acetate Esterase Activity of Peripheral Blood Lymphocytes in the Mallard, Muscovy and Pekin Ducks

Acta Veterinaria Brno ◽

10.2754/avb200675010033 ◽

2006 ◽

Vol 75 (1) ◽

pp. 33-38 ◽

Cited By ~ 4

Author(s):

T. Karaca ◽

M. Cemek ◽

M. Kanter

Keyword(s):

Lipid Peroxidation ◽

Vitamin C ◽

Peripheral Blood ◽

Serum Levels ◽

Peripheral Blood Lymphocytes ◽

Blood Levels ◽

Pekin Ducks ◽

Acetate Esterase ◽

Alpha Naphthyl Acetate Esterase ◽

Naphthyl Acetate

The aim of this study was to determine blood levels of malondialdehyde (MDA), reduced glutathione (GSH), ceruloplasmin and vitamin C, and the percentages of peripheral blood Tlymphocytes using the alpha-naphthyl acetate esterase (ANAE) method on Mallard (Anas platyrhynchos), Muscovy (Cairina moschata) and Pekin (Anas domestica) ducks. Blood samples were obtained from 8 adult ducks of each breed. The serum levels observed in Mallard, Muscovy and Pekin ducks respectively were 0.8, 1.07 and 1.3 nmol MDA per ml; 77.4, 66.9 and 78.7 mg GSH per 100 ml; 23.9, 26.1 and 24.1 mg ceruloplasmin per 100 ml; and 0.50, 0.52 and 0.70 mg vitamin C per 100 ml. The percentage of the ANAE (+) lymphocytes was 57.9%, 54.8% and 55.1% in Mallard, Muscovy and Pekin ducks, respectively. In this study, blood levels of lipid peroxidation and nonenzymatic antioxidants in the Mallard, Muscovy and Pekin ducks were determined.

Download Full-text

A method for the identification of human peripheral blood T lymphocytes by sequential immunogold and esterase double staining.

Journal of Histochemistry & Cytochemistry ◽

10.1177/31.6.6188784 ◽

1983 ◽

Vol 31 (6) ◽

pp. 837-839 ◽

Cited By ~ 9

Author(s):

V Bergroth ◽

Y T Konttinen ◽

S Reitamo

Keyword(s):

T Lymphocytes ◽

Peripheral Blood ◽

Human Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Mononuclear Leukocytes ◽

Double Staining ◽

Acetate Esterase ◽

Alpha Naphthyl Acetate Esterase ◽

Double Staining Method ◽

Naphthyl Acetate

A double staining method involving the sequential use of monoclonal OKT hybridoma antibodies applied in the colloidal immunogold method and followed by a simultaneously capturing azo dye method for the detection of acid alpha-naphthyl acetate esterase (ANAE) is described. Mononuclear leukocytes isolated from human peripheral blood using a Ficoll-Hypaque density gradient were stained. M-pattern ANAE-positive monocytes (diffuse staining) were excluded from the lymphocyte counts. 80 +/- 5% of all lymphocytes were T-pattern ANAE positive (dot-like staining) and 77 +/- 3% were OKT3 positive. 86 +/- 6% of all ANAE-positive T-pattern lymphocytes were also OKT3 positive, and 89 +/- 6% of all OKT3-positive lymphocytes were also ANAE positive. This indicates that ANAE is a good marker for total human T lymphocytes. 53 +/- 10% of human peripheral blood lymphocytes were OKT4 positive and 87 +/- 8% of all OKT4-positive lymphocytes were also ANAE positive. 30 +/- 6% of all lymphocytes were OKT8 positive, and only 26 +/- 18% of all OKT8-positive lymphocytes were ANAE negative. This indicates that ANAE cannot be used to distinguish T-helper and T-suppressor lymphocytes as identified by monoclonal antibodies.

Download Full-text