Acid alpha naphthyl acetate esterase reacting lymphocytes in the peripheral blood of chickens challenged with Marek’s disease after vaccination with three different vaccines

1987 ◽  
Vol 43 (1) ◽  
pp. 92-96 ◽  
Author(s):  
A.K. PRUTHI ◽  
R.K.P. GUPTA ◽  
J.R. SADANA
Keyword(s):  
Peripheral Blood ◽  
Marek's Disease ◽  
Marek’S Disease ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Naphthyl Acetate

scholarly journals Effects of Glucomannan on the Sacculus Rotundus and Peripheral Blood Lymphocytes in New Zealand Rabbits during Aflatoxicosis

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Emrah Sur ◽  
Hasan Hüseyin Dönmez ◽  
Murat Boydak ◽  
Mehmet Bozkurt Ataman
Keyword(s):  
New Zealand ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Negative Effects ◽  
Blood Lymphocytes ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Sacculus Rotundus ◽  
Naphthyl Acetate ◽  
New Zealand Rabbits

This study was aimed to determine the effects of the glucomannan added to aflatoxin- (AF-) contaminated diet on the sacculus rotundus and peripheral blood lymphocytes of New Zealand rabbits by histological and enzyme histochemical methods. Twenty-four adult rabbits of both sexes were divided into four equal groups, namely, as control, glucomannan 0.2 g/day, AF 125 μg/kg/day, and glucomannan combined with AF. The animals in all groups were treated for 12 weeks by the above-mentioned diet. When compared to control, AF-treatment caused significant decrease in alpha-naphthyl acetate esterase- (ANAE-) positive peripheral blood lymphocyte (PBL) percentages. The addition of the glucomannan to AFcontaining diet recovered the adverse effects of AF on sacculus rotundus and increased the ANAE-positive PBL counts. These results suggested that glucomannan was effective against the negative effects of AF in rabbits.

scholarly journals Effect of flumequine on peripheral blood leukocytes, alpha naphthyl acetate esterase activity and mitogenic lymphocyte response

2018 ◽  
Vol 74 (10) ◽  
pp. 653-657
Author(s):  
ANTONINA SOPIŃSKA ◽  
ANNA GROCHOŁA ◽  
MONIKA ROCZEŃ-KARCZMARZ ◽  
KRZYSZTOF TOMCZUK
Keyword(s):  
Peripheral Blood ◽  
Esterase Activity ◽  
Peripheral Blood Leukocytes ◽  
Con A ◽  
Blood Leukocytes ◽  
Group I ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Group Ii ◽  
Naphthyl Acetate

The aim of this study was to evaluate the effect of flumequine on the percentage of peripheral blood leukocytes of carp, alpha naphthyl acetate esterase (ANAE) activity and proliferative activity of lymphocytes stimulated with concanavalin A (Con A) and lipopolysaccharide (LPS). Flumequine was administered to 40 carp, weighing 150 ± 10 g, at a dose of 12 mg/kg, once (group I) and four times, every 2 days (group II). Among white blood cells in flumequine, treated fish (group II) observed a decrease in percentage of lymphocytes and an increase of neutrophiles. Identification of the ANAE esterase activity in fish lymphocytes of the control group showed the advantage of positive cells over the negative ones and amounted to 62.65 ± 3.22 %. After administration of flumequine in group II fish, this value decreased to 44.75 ± 3.70 %. The present study clearly demonstrated that both Con A and LPS induced lymphoid cell proliferation in vitro in group I. There was an increase in activity after stimulation of LPS and its reduction after Con A in group II. This indicates a stimulating effect of flumequine on B lymphocytes. The results of this study are not conclusive as to the positive effect of the drug on the immune system of fish and indicate the need for caution in its use. .

scholarly journals Lipid Peroxidation, Antioxidant Level, and Alpha Naphthyl Acetate Esterase Activity of Peripheral Blood Lymphocytes in the Mallard, Muscovy and Pekin Ducks

2006 ◽  
Vol 75 (1) ◽  
pp. 33-38 ◽  
Author(s):  
T. Karaca ◽  
M. Cemek ◽  
M. Kanter
Keyword(s):  
Lipid Peroxidation ◽  
Vitamin C ◽  
Peripheral Blood ◽  
Serum Levels ◽  
Peripheral Blood Lymphocytes ◽  
Blood Levels ◽  
Pekin Ducks ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Naphthyl Acetate

The aim of this study was to determine blood levels of malondialdehyde (MDA), reduced glutathione (GSH), ceruloplasmin and vitamin C, and the percentages of peripheral blood Tlymphocytes using the alpha-naphthyl acetate esterase (ANAE) method on Mallard (Anas platyrhynchos), Muscovy (Cairina moschata) and Pekin (Anas domestica) ducks. Blood samples were obtained from 8 adult ducks of each breed. The serum levels observed in Mallard, Muscovy and Pekin ducks respectively were 0.8, 1.07 and 1.3 nmol MDA per ml; 77.4, 66.9 and 78.7 mg GSH per 100 ml; 23.9, 26.1 and 24.1 mg ceruloplasmin per 100 ml; and 0.50, 0.52 and 0.70 mg vitamin C per 100 ml. The percentage of the ANAE (+) lymphocytes was 57.9%, 54.8% and 55.1% in Mallard, Muscovy and Pekin ducks, respectively. In this study, blood levels of lipid peroxidation and nonenzymatic antioxidants in the Mallard, Muscovy and Pekin ducks were determined.

scholarly journals A method for the identification of human peripheral blood T lymphocytes by sequential immunogold and esterase double staining.

1983 ◽  
Vol 31 (6) ◽  
pp. 837-839 ◽  
Author(s):  
V Bergroth ◽  
Y T Konttinen ◽  
S Reitamo
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Mononuclear Leukocytes ◽  
Double Staining ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Double Staining Method ◽  
Naphthyl Acetate

A double staining method involving the sequential use of monoclonal OKT hybridoma antibodies applied in the colloidal immunogold method and followed by a simultaneously capturing azo dye method for the detection of acid alpha-naphthyl acetate esterase (ANAE) is described. Mononuclear leukocytes isolated from human peripheral blood using a Ficoll-Hypaque density gradient were stained. M-pattern ANAE-positive monocytes (diffuse staining) were excluded from the lymphocyte counts. 80 +/- 5% of all lymphocytes were T-pattern ANAE positive (dot-like staining) and 77 +/- 3% were OKT3 positive. 86 +/- 6% of all ANAE-positive T-pattern lymphocytes were also OKT3 positive, and 89 +/- 6% of all OKT3-positive lymphocytes were also ANAE positive. This indicates that ANAE is a good marker for total human T lymphocytes. 53 +/- 10% of human peripheral blood lymphocytes were OKT4 positive and 87 +/- 8% of all OKT4-positive lymphocytes were also ANAE positive. 30 +/- 6% of all lymphocytes were OKT8 positive, and only 26 +/- 18% of all OKT8-positive lymphocytes were ANAE negative. This indicates that ANAE cannot be used to distinguish T-helper and T-suppressor lymphocytes as identified by monoclonal antibodies.

scholarly journals Chromosome pattern in childhood acute nonlymphocytic leukemia (ANLL)

Blood ◽  
1982 ◽  
Vol 60 (2) ◽  
pp. 389-399 ◽  
Author(s):  
Y Kaneko ◽  
JD Rowley ◽  
HS Maurer ◽  
D Variakojis ◽  
JW Moohr
Keyword(s):  
Age Groups ◽  
Normal Karyotype ◽  
Chromosome Abnormalities ◽  
Host Susceptibility ◽  
Leukemic Cells ◽  
Karyotypic Analysis ◽  
Chromosome Pattern ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Naphthyl Acetate

Abstract We studied the karyotype in 26 children with ANLL, which was diagnosed on the basis of the FAB classification. Clonal chromosome abnormalities were found in 21 of 26 patients. Four patients, including 3 with Down's syndrome, had AML(M1). Nine patients, including 3 with t(8;21), had AML(M2). All 3 patients with APL(M3) had t(15;17). Four patients had AMMOL(M4); 3 of these had a normal karyotype. Six patients had AMOL(M5); 5 and 11q rearrangements, and 3 of these had a break in 11q23. Only one patient had EL(M6), and he had a normal karyotype. One patient with t(11;19), classified as AML(M2) on Wright-Giemsa-stained cells, had a strong alpha-naphthyl acetate esterase reaction, indicating that the leukemic cells had a cytochemical feature characteristic of monocytes. Whereas t(8;21) and t(15;17) are uniquely associated with AML(M2) and APL(M3), respectively, the 11q rearrangements are also seen in AML(M1/M2), although they are more common in AMOL(M5) and AMMOL(M4). The case with t(11;19) suggests that cells with 11q rearrangements and with AML(M1/M2) may have both monocytic and granulocytic features. When we used our data and previous reports on 243 aneuploid patients (169 adults and 74 children) to correlate the chromosome abnormalities with patient age, we found differences in the chromosome pattern seen among various age groups. This suggests that different etiologic factors as well as changes in host susceptibility may influence the development of and the karyotypic pattern in the various types of leukemia. Moreover, the frequency of various chromosome abnormalities in childhood ANLL can provide a baseline for comparison of the frequency of the same abnormality in adults. The karyotypic analysis of childhood ANLL is important not only because of the information that can be obtained about childhood ANLL, but also because the data can provide substantial insight into the etiology of ANLL in adults.

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