scholarly journals Effects of Glucomannan on the Sacculus Rotundus and Peripheral Blood Lymphocytes in New Zealand Rabbits during Aflatoxicosis

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Emrah Sur ◽  
Hasan Hüseyin Dönmez ◽  
Murat Boydak ◽  
Mehmet Bozkurt Ataman
Keyword(s):  
New Zealand ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Negative Effects ◽  
Blood Lymphocytes ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Sacculus Rotundus ◽  
Naphthyl Acetate ◽  
New Zealand Rabbits

This study was aimed to determine the effects of the glucomannan added to aflatoxin- (AF-) contaminated diet on the sacculus rotundus and peripheral blood lymphocytes of New Zealand rabbits by histological and enzyme histochemical methods. Twenty-four adult rabbits of both sexes were divided into four equal groups, namely, as control, glucomannan 0.2 g/day, AF 125 μg/kg/day, and glucomannan combined with AF. The animals in all groups were treated for 12 weeks by the above-mentioned diet. When compared to control, AF-treatment caused significant decrease in alpha-naphthyl acetate esterase- (ANAE-) positive peripheral blood lymphocyte (PBL) percentages. The addition of the glucomannan to AFcontaining diet recovered the adverse effects of AF on sacculus rotundus and increased the ANAE-positive PBL counts. These results suggested that glucomannan was effective against the negative effects of AF in rabbits.

scholarly journals Lipid Peroxidation, Antioxidant Level, and Alpha Naphthyl Acetate Esterase Activity of Peripheral Blood Lymphocytes in the Mallard, Muscovy and Pekin Ducks

2006 ◽  
Vol 75 (1) ◽  
pp. 33-38 ◽  
Author(s):  
T. Karaca ◽  
M. Cemek ◽  
M. Kanter
Keyword(s):  
Lipid Peroxidation ◽  
Vitamin C ◽  
Peripheral Blood ◽  
Serum Levels ◽  
Peripheral Blood Lymphocytes ◽  
Blood Levels ◽  
Pekin Ducks ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Naphthyl Acetate

The aim of this study was to determine blood levels of malondialdehyde (MDA), reduced glutathione (GSH), ceruloplasmin and vitamin C, and the percentages of peripheral blood Tlymphocytes using the alpha-naphthyl acetate esterase (ANAE) method on Mallard (Anas platyrhynchos), Muscovy (Cairina moschata) and Pekin (Anas domestica) ducks. Blood samples were obtained from 8 adult ducks of each breed. The serum levels observed in Mallard, Muscovy and Pekin ducks respectively were 0.8, 1.07 and 1.3 nmol MDA per ml; 77.4, 66.9 and 78.7 mg GSH per 100 ml; 23.9, 26.1 and 24.1 mg ceruloplasmin per 100 ml; and 0.50, 0.52 and 0.70 mg vitamin C per 100 ml. The percentage of the ANAE (+) lymphocytes was 57.9%, 54.8% and 55.1% in Mallard, Muscovy and Pekin ducks, respectively. In this study, blood levels of lipid peroxidation and nonenzymatic antioxidants in the Mallard, Muscovy and Pekin ducks were determined.

scholarly journals A method for the identification of human peripheral blood T lymphocytes by sequential immunogold and esterase double staining.

1983 ◽  
Vol 31 (6) ◽  
pp. 837-839 ◽  
Author(s):  
V Bergroth ◽  
Y T Konttinen ◽  
S Reitamo
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Mononuclear Leukocytes ◽  
Double Staining ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Double Staining Method ◽  
Naphthyl Acetate

A double staining method involving the sequential use of monoclonal OKT hybridoma antibodies applied in the colloidal immunogold method and followed by a simultaneously capturing azo dye method for the detection of acid alpha-naphthyl acetate esterase (ANAE) is described. Mononuclear leukocytes isolated from human peripheral blood using a Ficoll-Hypaque density gradient were stained. M-pattern ANAE-positive monocytes (diffuse staining) were excluded from the lymphocyte counts. 80 +/- 5% of all lymphocytes were T-pattern ANAE positive (dot-like staining) and 77 +/- 3% were OKT3 positive. 86 +/- 6% of all ANAE-positive T-pattern lymphocytes were also OKT3 positive, and 89 +/- 6% of all OKT3-positive lymphocytes were also ANAE positive. This indicates that ANAE is a good marker for total human T lymphocytes. 53 +/- 10% of human peripheral blood lymphocytes were OKT4 positive and 87 +/- 8% of all OKT4-positive lymphocytes were also ANAE positive. 30 +/- 6% of all lymphocytes were OKT8 positive, and only 26 +/- 18% of all OKT8-positive lymphocytes were ANAE negative. This indicates that ANAE cannot be used to distinguish T-helper and T-suppressor lymphocytes as identified by monoclonal antibodies.

Selective COX-2 Inhibition Exerts No Negative Effects on Peripheral Blood Lymphocytes in Allergic Asthmatics

2016 ◽  
Vol 170 (1) ◽  
pp. 57-61
Author(s):  
Guro Gafvelin ◽  
Jeanette Grundström ◽  
Maria Edin Grimheden ◽  
Sara Sánchez Vidaurre ◽  
Kameran Daham ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Negative Effects ◽  
Blood Lymphocytes ◽  
Cox 2

scholarly journals Effect of flumequine on peripheral blood leukocytes, alpha naphthyl acetate esterase activity and mitogenic lymphocyte response

2018 ◽  
Vol 74 (10) ◽  
pp. 653-657
Author(s):  
ANTONINA SOPIŃSKA ◽  
ANNA GROCHOŁA ◽  
MONIKA ROCZEŃ-KARCZMARZ ◽  
KRZYSZTOF TOMCZUK
Keyword(s):  
Peripheral Blood ◽  
Esterase Activity ◽  
Peripheral Blood Leukocytes ◽  
Con A ◽  
Blood Leukocytes ◽  
Group I ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Group Ii ◽  
Naphthyl Acetate

The aim of this study was to evaluate the effect of flumequine on the percentage of peripheral blood leukocytes of carp, alpha naphthyl acetate esterase (ANAE) activity and proliferative activity of lymphocytes stimulated with concanavalin A (Con A) and lipopolysaccharide (LPS). Flumequine was administered to 40 carp, weighing 150 ± 10 g, at a dose of 12 mg/kg, once (group I) and four times, every 2 days (group II). Among white blood cells in flumequine, treated fish (group II) observed a decrease in percentage of lymphocytes and an increase of neutrophiles. Identification of the ANAE esterase activity in fish lymphocytes of the control group showed the advantage of positive cells over the negative ones and amounted to 62.65 ± 3.22 %. After administration of flumequine in group II fish, this value decreased to 44.75 ± 3.70 %. The present study clearly demonstrated that both Con A and LPS induced lymphoid cell proliferation in vitro in group I. There was an increase in activity after stimulation of LPS and its reduction after Con A in group II. This indicates a stimulating effect of flumequine on B lymphocytes. The results of this study are not conclusive as to the positive effect of the drug on the immune system of fish and indicate the need for caution in its use. .

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