Effects of carbamate pesticides intermediates on Escherichia coli membrane architecture: An in vitro and in silico approach

Methyl isocyanate (MIC), a low molecular weight synthetic aliphatic compound, having an isocyanate group (−NCO), has industrial application. In this study, the effects of methyl isocyanate and its mechanism on outer membrane protein of Escherichia coli were observed using experimental and computational methods. In vitro exposure of N-succinimidyl N-methylcarbamate (NSNM) a synthetic analogue of MIC on E. coli to a final concentration of 2 mM was found to affect the growth curve pattern and changes in cell morphology. Molecular docking studies of MIC and NSNM with E. coli outer membrane protein (OmpW, OmpX, OmpF OmpA), and periplasmic domain (PAL) were performed. The in-silico results revealed that outer membrane protein OmpF showed the highest negative binding energy, i.e. ∆G -4.11 kcal/mole and ∆G -3.19 kcal/mole by NSNM and MIC as compared to other proteins. Our study concludes that methyl isocyanate retains lethal toxicity which leads to cell death due to the membrane protein damage of E. coli membrane.

Susceptibility of the Bhopal–methyl isocyanate (MIC)–gas–tragedy survivors and their offspring to COVID-19: What we know, what we don’t and what we should?

There is credible evidence that the 1984–Bhopal–methyl isocyanate (MIC)–gas–exposed long-term survivors and their offspring born post-exposure are susceptible to infectious/communicable and non-communicable diseases. Bhopal’s COVID-19 fatality rate suggests that the MIC–gas tragedy survivors are at higher risk, owing to a weakened immune system and co-morbidities. This situation emboldened us to ponder over what we know, what we don’t, and what we should know about their susceptibility to COVID-19. This article aims at answering these three questions that emerge in the minds of public health officials concerning prevention strategies against COVID-19 and health promotion in the Bhopal MIC-affected population (BMAP). Our views and opinions presented in this article will draw attention to prevent and reduce the consequences of COVID-19 in BMAP. From the perspective of COVID-19 prophylaxis, the high-risk individuals from BMAP with co-morbidities need to be identified through a door-to-door visit to the severely gas-affected regions and advised to maintain good respiratory hygiene, regular intake of immune-boosting diet, and follow healthy lifestyle practices.

PREVENTING THE TRANSITION OF CORONA THROUGH SCIENCE & TECHNOLOGY:GLOBALLY INTEGRATED SOCIETY TOWARDS POSITIVE CHANGE

The entire basis of science is technology, technology that facilitates us, it also cautions us how destructive it can be to use it wrongly. Such as Bhopal Gas Tragedy containing leakage of Methyl isocyanate or radiation from Japan’s atomic reactor due to Tsunami, it is an event that the slightest negligence of science or technology can lead to a major tragedy.Covid-19 is one such phenomenon, and it took a while to understand and it convert into form of a serious illness. The Pandemic Disease of the century, which is spreading its infection in the whole world, and is likely to be Seriously thought.