Identification of lipase producing bacteria from palm oil sewage sludge processing plant at Malimping, Banten, Indonesia

Layli IR, Meryandini A, Helianti I, Astuti RI. 2021. Identification of lipase-producing bacteria from palm oil sewage sludge processing plant at Malimping, Banten, Indonesia. Biodiversitas 22: 4512-4524. Lipase (triacylglycerol hydrolase E.C.3.1.1.3) is an enzyme that catalyzes the hydrolysis reaction of triacylglycerol into free fatty acids and glycerols. Lipase has broad application spectrum and unique catalytic mechanism, as one of important enzymes for industries, and almost all enzymes used for industries are originated from microbes. Identification of lipase-producing bacteria from palm oil processing sewage sludge was carried out to determine the potential of isolates and the application of lipase enzymes for industry. This study identified lipase-producing bacteria from the screening and isolation results from palm oil sewage sludge in Malimping-Banten. Two isolates were obtained from screening and isolation results. The morphological and observation and Gram staining method resulted in mlp-1 colony isolate was Gram-negative with basil cell shape, while mlp-2 colony isolate was Gram-positive with basil cell shape. Qualitative test on two isolates in tributyrin agar showed that two isolates produced clear zone or lipase enzyme. Molecular identification using 16SrRNA sequence showed that mlp-1 colony isolate was Chryseobacterium gleum, while mlp-2 colony was Bacillus velezensis. Literature study stated that Chryseobacterium gleum are pathogenic bacteria, resulting in uncontinued identification process, while Bacillus velezensis are non-pathogenic bacteria that have potential for industrial application. Identification of Bacillus velezensis was continued by performing biochemical test and inhibitory test. The continued identification results supported the molecular identification of lipase-producing enzyme for Bacillus velezensis.

Isolation and selection of nitrite metabolising bacteria from the bottom mud of lobster culture area in Xuan Dai bay, Phu Yen province

The study had isolated and selected groups of bacteria that metabolise nitrite from the bottom mud of lobster cages in Xuan Dai bay, Phu Yen province. Analysis results from 21 sludge samples taken from 11 cages of lobster farming area isolated 16 strains of bacteria capable of nitrite metabolism. After investigating biological characteristics and nitrite metabolism of bacteria strains, 10 strains of bacteria were collected with the ability to metabolise nitrite over 95% in 72 hours. In addition, 10 strains of bacteria with the highest NO2- treatment efficiency, identified by genetic analysis and looked up on BLAST, defined as Stenotrophomonas pavanii, Chryseobacterium gleum, Stenotrophomonas maltophilia, Delftia lacustris, Acinetobacter junii

Multiple Drug-Resistant CLABSI from an Extremely Rare Bacterium, Chryseobacterium gleum

Chryseobacterium gleum is a lactose nonfermenting Gram-negative bacillus (NFGNB) found in soil, plants, and some water sources but rarely implicated as a human pathogen. Its scarcity in the medical literature and resistance to numerous broad-spectrum antibiotics such as carbapenems, cephalosporins, and beta-lactam/lactamase inhibitors pose a diagnostic and therapeutic challenge. We present the first reported case, to the best of our knowledge, of sepsis from central line-associated blood stream infection from Chryseobacterium gleum in the United States.