Isolation and selection of nitrite metabolising bacteria from the bottom mud of lobster culture area in Xuan Dai bay, Phu Yen province

The study had isolated and selected groups of bacteria that metabolise nitrite from the bottom mud of lobster cages in Xuan Dai bay, Phu Yen province. Analysis results from 21 sludge samples taken from 11 cages of lobster farming area isolated 16 strains of bacteria capable of nitrite metabolism. After investigating biological characteristics and nitrite metabolism of bacteria strains, 10 strains of bacteria were collected with the ability to metabolise nitrite over 95% in 72 hours. In addition, 10 strains of bacteria with the highest NO2- treatment efficiency, identified by genetic analysis and looked up on BLAST, defined as Stenotrophomonas pavanii, Chryseobacterium gleum, Stenotrophomonas maltophilia, Delftia lacustris, Acinetobacter junii

Adsorption and migration of heavy metals between sediments and overlying water in the Xinhe River in central China

Long-term polluted rivers often lead to the accumulation of heavy metals in sediments. Anthropogenic activities or biological disturbances break the adsorption balance, causing them to return from the bottom mud to the overlying water and change the aquatic environment. In order to understand the variation of heavy metals between sediments and river water, we collected the riverbed sediments in the polluted Xinhe River and carried out static continuous infiltration and dynamic uninterrupted disturbance experiments. The leaching experiment shows that the absorbability of Cd and Pb is stronger than Cr in the sediment; at the same time, the properties of the medium have a great influence on the adsorption of heavy metals. The disturbance can prompt heavy metals in the sediment to resuspend into the overlying water. The impact is the greatest during the first 12 h, and the influence degree is stronger in the relatively static water than in the moving river. In addition, pH and other factors have different degrees of influence on the desorption of heavy metals.

Embryonic developmental arrest in the annual killifish Austrolebias charrua: A proteomic approach to diapause III

Diapause is a reversible developmental arrest faced by many organisms in harsh environments. Annual killifish present this mechanism in three possible stages of development. Killifish are freshwater teleosts from Africa and America that live in ephemeral ponds, which dry up in the dry season. The juvenile and adult populations die, and the embryos remain buried in the bottom mud until the next rainy season. Thus, species survival is entirely embryo-dependent, and they are perhaps the most remarkable extremophile organisms among vertebrates. The aim of the present study was to gather information about embryonic diapauses with the use of a “shotgun” proteomics approach in diapause III and prehatching Austrolebias charrua embryos. Our results provide insight into the molecular mechanisms of diapause III. Data are available via ProteomeXchange with identifier PXD025196. We detected a diapause-dependent change in a large group of proteins involved in different functions, such as metabolic pathways and stress tolerance, as well as proteins related to DNA repair and epigenetic modifications. Furthermore, we observed a diapause-associated switch in cytoskeletal proteins. This first glance into global protein expression differences between prehatching and diapause III could provide clues regarding the induction/maintenance of this developmental arrest in A. charrua embryos. There appears to be no single mechanism underlying diapause and the present data expand our knowledge of the molecular basis of diapause regulation. This information will be useful for future comparative approaches among different diapauses in annual killifish and/or other organisms that experience developmental arrest.

Study on enhanced bioremediation effect of oil-bearing dredging sediment

To solve the problem of difficult treatment of oil-bearing dredging sediment, the effects of three methods of microbial remediation, phytoremediation and combined bioremediation on the treatment of oily river dredging sediments were compared and studied, and the influence factors of microbial remediation were explored through static bacteria-fling tests. The results showed that the combined remediation method was better than the single biological method in treating bottom mud. The oil content of the treated sediment was 1.21g/kg, and the removal rate was 95.31%; the organic matter of the treated sediment was 72.30g/kg, and the degradation rate was 37.33. %, which can meet the requirements in the “Control Standards of Pollutants in Sludge for Agricultural Use (GB4284–2018)” and can be used for agricultural purposes.

PEMANFAATAN LOKASI TAMBAK KAWASAN ALUE NAGA ACEH BESAR UNTUK PENGEMBANGAN BUDIDAYA RUMPUT LAUT JENIS GLACILARIA MENGGUNAKAN FLOATING HOUSE METHOD DALAM MENDUKUNG KETAHANAN PANGAN

Aceh Province is an area surrounded by coasts which has great potential to develop marine product cultivation, one of which is seaweed. In line with the goal of the central government in 2018 stating its support for increaseing seaweed cultivation in Aceh Province. Meanwhile, the Ministry of Maritime Affairs and Fisheries' Directorate General of Cultivation stated that so far the seaweed industry and cultivation are still constrained by transportation problems for marketing. Based on the results of research in a number of regions in Indonesia, it is explained that the coastal area of Aceh is an area that has very good potential for the seaweed cultivation. This study focuses on the Glacilaria sp seaweed cultivation due to its easiness to grow in community ponds. The drawback of this pond cultication method is the existence of predators that eat seaweed and the pond bottom mud that pollutes the seaweed. The application of floating house technology for glacilaria seaweed culture is seen as a solution for aforemention problems. This technology protects the seaweed from predators and the contamination of the seawee from mud pond. The proposed sea seaweed cultivation method is anticipated to offer better income and improve the quality of life of the community, especially in the the targeted area, Alue Naga. Regarding the current situation, Farmer in Alue Naga still practice the conventional method for cultivating the seaweed in the land pond. This regular method has the shortcoming in protecting the seeweed from the predators. In addition, due to the seaweed attached to the bottom of the pond, it causes the slow growth of seaweed. These circumstances cause the crops have lower quality, result in a hardly marketable product either for national or international markets. To cope with such conditions, an easy to use, reliable and safety floating house was proposed and built for the use of glacilaria seaweed culture. The community is also engaged in the workshop and construction of floating house for the glacilaria seaweed culture taught by staff of Cot Irieh Fiberlass workshop. This engagement aims to increase the insight and skill of the community regarding construction and maintenance of the glacilaria seaweed culture floating house. This study is fully supported by Syiah Kuala University Research and Community Service Institute through fostering the Alue Naga fishing community through direct application of technology.

Effects of substrate and water depth of a eutrophic pond on the physiological status of a submerged plant, Vallisneria natans

Effects of substrate and water depth on the physiological status of a submerged macrophyte, Vallisneria natans (Lour.) H. Hara, were determined by measuring biomarkers in leaves and roots, to understand factors limiting the re-establishment of V. natans in urban eutrophic ponds. Ramets of V. natans were grown in the laboratory using aquaria containing water and bottom mud from a eutrophic pond and maintained under sufficient light in an incubator. The growth and chlorophyll-a (Chl-a) content of leaves were greater in aquaria with mud than in those with sand, which was used as the reference substrate. The contents of a peroxidation product (malondialdehyde (MDA)) and three antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD)) in leaves and roots, used as stress biomarkers, changed during the experiment, although differences in these contents between mud and sand were not consistent across the experimental days. To control water depth in the field, ramets of V. natans were grown in cages with different substrates (mud and sand) installed at different depths (0.5, 1.2, and 2.0 m) in the pond. The mean light quantum during the experiment decreased with increasing depth, from 79.3 μmol/m2 s at 0.5 m to 7.9 μmol/m2 s at 2.0 m. The Chl-a content in leaves decreased, whereas the MDA content in both leaves and roots increased with increasing water depth. All enzyme activities increased at the beginning and then decreased to the end of the experiment at 2.0 m depth, suggesting deterioration of enzyme activities due to depth-related stress. The MDA content and CAT activity were higher for sand than for mud, whereas the difference in the growth and the leaf Chl-a content between substrates remained unclear in the pond. On comparing the laboratory and field experiments, the leaf Chl-a content was found to be lower and the MDA content and enzyme activities exhibited sharp increase for ramets grown in the pond, even at 0.5 m depth, when compared with those grown in the aquaria. Our results suggest that the bottom mud of the pond is not the major limiting factor in the re-establishment of V. natans. Because water depth and light attenuation exerted strong stress on V. natans, shallow areas or measures to improve water transparency are required to promote the introduction of V. natans in eutrophic ponds for successful restoration in urban areas.

Dynamic Interception Effect of Internal and External Nitrogen and Phosphorus Migration of Ecological Ditches

The “ecological ditch” (eco-ditch) is an effective measure used to alleviate agricultural non-point-source pollution. However, information is lacking about the continuous transport characteristics of internal and external nitrogen and phosphorus in the interstitial water of the bottom mud of these ditches and overlying water under dynamic continuous inflow conditions. Understanding of the effect of matrix dams and microbial communities inside eco-ditches on the continuous transport characteristics of the N and P therein needs to be improved. To determine the interception effects of eco-ditches on the transfer of endogenous and exogenous N and P, an eco-ditch combining plants and a matrix dam was built to explore the transport distribution characteristics of N and P in the intermittent water and overlying water in the bottom of the eco-ditch and in the bottom of the soil ditch. We compared and analyzed the composition characteristics of the microbiological communities along the ecological and soil ditches. The research results showed that: (1) The concentration gradient between the interstitial water and the overlying water in the soil ditch is the main reason for the transport and diffusion of pollutants. However, in eco-ditches, the absorption function of plant roots and the differences between the structures of the microbial communities destroy the correlation of this concentration gradient diffusion, especially the effect on ammonium N; (2) a large number of mycelia adhere to the surface of the matrix dam in an eco-ditch, and are conducive to the adsorption and purification of pollutants in the water; (3) Proteobacteria, Chloroflexi, Actinomycetes, and Acidobacteria were the main bacterial groups in the ditches. The aquatic plants in the eco-ditch changed the microenvironment of the sediment, and both the microbial diversity and abundance along the eco-ditch were higher than in the soil ditch.

Geoheritage Resource of Small Mud Lakes in the Semi-Arid Environments of the Russian South

Lakes constitute a significant freshwater resource and are often regarded as being an important part of a country’s natural heritage. However, their geological heritage (geoheritage) value remains significantly understudied. Three small lakes, namely the Salt Lake, the Gruzskoe Lake and the Big Yashalta Lake, located in the central part of the Russian South were studied in this paper. The main focus of this research was to search for relevant unique geological features. This paper established that these lakes are distinguishable from other lakes by the presence of dark-colored mud that is rich in sulfides and methane. As a result of excessive summer evaporation, the lakes desiccate (partly or even fully) and this results in exposure of the mud as well as the formation of salt crystals and crusts. This bottom mud (peloid, therapeutic mud) forms as a result of highly-complex biogeochemical processes in semi-arid environments. This mud is interpreted as being part of the lake’s geoheritage as it belongs to sedimentary, geochemical and other types. Moreover, this geoheritage has a high ranking nationally. The most representative example can be found at the Big Yashalta Lake and for that reason, this lake is proposed as a geosite. Established sites of geoheritage importance are vital to geoscience research and geotourism activities and thus, these sites are considered to be a resource. Together with the development of local ecotourism and the use of peloids for the health industry purposes, the exploitation of this geoheritage resource would contribute to the sustainable development of the local area.