Review on in vivo profiling of drug metabolites with LC-MS/MS in the past decade

Metabolite profiling is an indispensable part of drug discovery and development, enabling a comprehensive understanding of the drug's metabolic behavior. Liquid chromatography-mass spectrometry facilitates metabolite profiling by reducing sample complexity and providing high sensitivity. This review discusses the in vivo metabolite profiling involving LC-MS/MS and the utilization of QTOF, QQQ mass analyzers with a particular emphasis on a mass filter. Further, a summary of sample extraction procedures in biological matrices such as plasma, urine, feces, serum and hair as in vivo samples are outlined. toward the end, we present 15 case studies in biological matrices and their LC-MS/MS conditions to understand the metabolic disposition.

SPATIAL SEPARATION OF THE IONES OF A GIVEN MASS RANGE IN THE DEMO-IMITATION SEPARATOR AT THE FIRST TURN OF IONIC TRAJECTORY

Plasma methods, where only electricity is required, are an alternative to the PUREX process used in industry for spent nuclear fuel reprocessing. It is considered the possibility of filtering out the target ions (М = 232…277) to the collector at the first turn of the ion trajectory in the plasma mass filter, which is currently being developed, that is achieved by specifying certain parameters (amplitude and frequency) for a variable component of a radial electric field. This approach significantly reduces the thermal load onto deposition surface of target ions.

In situ Rb-Sr dating by collision cell, multicollection inductively-coupled plasma mass-spectrometry with pre-cell mass-filter, (CC-MC-ICPMS/MS).

We document the utility for in situ Rb-Sr dating of a one-of-a-kind tribrid mass spectrometer, ‘Proteus’, coupled to a UV laser ablation system. Proteus combines a pre-cell quadrupole mass-filter,collision cell,...

Low-Vacuum Quadrupole Mass Filter Using a Drift Gas

Performing mass spectrometry in a low-vacuum environment can markedly reduce the cost, size, and power consumption of instrumentation by reducing the workload of the pumping system. Under a low-vacuum environment, ions in a quadrupole mass filter do not have sufficient kinetic energy in the axial direction to reach the detector for mass analysis. To resolve this problem and develop a mass spectrometer suitable for a low-vacuum environment, a mass analysis method is proposed where a drift gas is used to supply energy to the ions. A simulation model was constructed in COMSOL Multiphysics, and a simple experimental device was built to validate the proposed method. The simulation results showed that this method effectively solves these problems, and the obtained spectral peak was superior to that without drift gas flow regarding spectral peak intensity and width. The experimental results showed that the proposed method separated ions with different mass-to-charge ratios at a pressure of 20 Pa. This work provides a theoretical foundation for the development of low-vacuum mass spectrometry, which will promote portability, provide a lower threshold of use, and expand the fields of application for mass spectrometers.