Effect of Environmental Contaminant on Rana nigromaculata Based on Environmental Materials

Environmental materials are widely used, which destroy the environment. We used Rana nigromaculata to research the effects of environmental material dicofol. Rana nigromaculata were injected in ventral lymph follicle in different doses of dicofol solution every a day. After the treatment, the sperm activation, sperm deformity, number and abnormal ratio were observed and calculated. The results show that with the dicofol concentration increasing, sperm activation reduces, sperm number decreases, sperm abnormality rate increases significantly. The conclusion is that dicofol has toxic effects on R. nigromaculata reproduction obviously.

Amphibian as a Model to Study Environmental Problem

In order to obtain a better insight of the possible threat of environmental factor to wildlife, especially to amphibians, we studied influences of dicofol on heart rate inR. nigromaculataby applying biological signal recording and monitoring system.R. nigromaculatawas injected in ventral lymph follicle in different doses of dicofol solution, and then observed the changes of heart rate after 7 days. The results showed that with the concentration of dicofol solution increasing, the heart rate, systolic tension and diastolic tension changed. The aim of this research is to discuss the cause of dicofol on the physiology in frogs. It can be concluded that dicofol affects the electronic activity of the frog’s heart.