“The Light at the End of the Tunnel Has Been Permanently Shut Off”: Work-Role Overload Among U.S. Police

The functional breadth of the police role is a primary issue facing law enforcement. However, few empirical data examine how officers are experiencing an occupational environment characterized by an increasingly wider range of new (but routine) duties. I take a qualitative approach to explore experiences of work-role overload via in-depth, semi-structured interviews with a sample of U.S. police officers ( N = 48). By applying the framework for thematic analysis, I find that work-role overload is a robust feature of police officers’ occupational experiences and presents in two ways: (a) through quantitative overload related to the excessive volume of work demands and (b) qualitative overload related to strained or diminished psychological resources. The findings provide valuable insights for improving the theoretical understanding of work-role overload among police in light of international trends toward broadening law enforcement’s social functions and add to contemporary discussions to “defund the police.”

T cell and antibody functional correlates of severe COVID-19

ABSTRACTComorbid medical illnesses, such as obesity and diabetes, are associated with more severe COVID-19, hospitalization, and death. However, the role of the immune system in mediating these clinical outcomes has not been determined. We used multi-parameter flow cytometry and systems serology to comprehensively profile the functions of T cells and antibodies targeting spike, nucleocapsid, and envelope proteins in a convalescent cohort of COVID-19 subjects who were either hospitalized (n=20) or not hospitalized (n=40). To avoid confounding, subjects were matched by age, sex, ethnicity, and date of symptom onset. Surprisingly, we found that the magnitude and functional breadth of virus-specific CD4 T cell and antibody responses were consistently higher among hospitalized subjects, particularly those with medical comorbidities. However, an integrated analysis identified more coordination between polyfunctional CD4 T-cells and antibodies targeting the S1 domain of spike among subjects that were not hospitalized. These data reveal a functionally diverse and coordinated response between T cells and antibodies targeting SARS-CoV-2 which is reduced in the presence of comorbid illnesses that are known risk factors for severe COVID-19. Our data suggest that isolated measurements of the magnitudes of spike-specific immune responses are likely insufficient to anticipate vaccine efficacy in high-risk populations.

Functional Responsibilities of Municipal Governments

This article examines the assignment of functional responsibilities to municipalities, contributing conceptualization and measurement for the analysis of breadth of those responsibilities across the American states. It also investigates determinants of functional breadth: alternative explanations are explored in an analysis of municipalities in metropolitan areas. Using data from the 2012 Census of Governments, two measures of functional breadth are reported, thus evaluating the reliability of the findings across alternative measurements of breadth. The main findings are that a diverse scope of functional responsibilities is prevalent across American municipalities and that the institutional environment of municipalities influences those diverse functional responsibilities. Additional findings are that certain factors differentially affect the scope of service responsibilities, according to a quantile analysis of the dependent variable’s distribution. Although its primary contribution is to the literature on functional responsibilities of governments, the article also proposes a political market approach to identify factors influencing functional responsibilities.

Independent activation of ion conduction pores in the double-barreled calcium-activated chloride channel TMEM16A

The TMEM16 proteins constitute a family of membrane proteins with unusual functional breadth, including lipid scramblases and Cl− channels. Members of both these branches are activated by Ca2+, acting from the intracellular side, and probably share a common architecture, which was defined in the recent structure of the lipid scramblase nhTMEM16. The structural features of subunits and the arrangement of Ca2+-binding sites in nhTMEM16 suggest that the dimeric protein harbors two locations for catalysis that are independent with respect to both activation and lipid conduction. Here, we ask whether a similar independence is observed in the Ca2+-activated Cl− channel TMEM16A. For this purpose, we generated concatenated constructs containing subunits with distinct activation and permeation properties. Our biochemical investigations demonstrate the integrity of concatemers after solubilization and purification. During investigation by patch-clamp electrophysiology, the functional behavior of constructs containing either two wild-type (WT) subunits or one WT subunit paired with a second subunit with compromised activation closely resembles TMEM16A. This resemblance extends to ion selectivity, conductance, and the concentration and voltage dependence of channel activation by Ca2+. Constructs combining subunits with different potencies for Ca2+ show a biphasic activation curve that can be described as a linear combination of the properties of its constituents. The functional independence is further supported by mutation of a putative pore-lining residue that changes the conduction properties of the mutated subunit. Our results strongly suggest that TMEM16A contains two ion conduction pores that are independently activated by Ca2+ binding to sites that are embedded within the transmembrane part of each subunit.