Counting individual bacteria during high-throughput growth in microfluidic droplets

Bacterial growth in microfluidic droplets is relevant in biotechnology, in microbial ecology, and in understanding stochastic population dynamics in small populations. However, it has proved challenging to automate measurement of absolute bacterial numbers within droplets, forcing the use of proxy measures for population size. Here we present a microfluidic device and imaging protocol that allows high-resolution imaging of thousands of droplets, such that individual bacteria stay in the focal plane and can be counted automatically. We demonstrate the use of this approach to track stochastic growth of multiple replicate Escherichia coli populations within droplets.

Perancangan Purwarupa Sistem Pengendalian Kualitas Pengukuran Dimensi Produk Terotomasi

The production process is one of the several main activities in industries. The process followed by quality control to achieve expected final products. Products' conformities to design specification can be achieved through direct measurement. However, not all measurements can be done directly while the process is running. Inconformities to product's design specification could result in unfunctional or rejected products. This will render producers into losing their profits. Technology plays a major role in simplifying products' measurement processes. Advancement in technology made it possible to automate measurement processes and will also make it possible to do during the production process in achieving expected quality control as early as possible. The quality control yield measurements in products'lengths, widths and depths/ heights which will control products' dimension and generate quality control chart. The proposed prototype will allow measurements of product dimension and data acquisition directly during the process without disrupting on going processes.