Mimosmluvní závazkové poměry s mezinárodním prvkem z pohledu české autonomní úpravy

Private International Law is nowadays, to a large extent, regulated by European and international law and the scope of the application of autonomous national law is therefore limited. However, in case of non-contractual obligations with cross-border elements, this scope is still relatively broad, as certain matters are excluded from the regulation on the European and international level. The aim of this article is to analyze the regulation of non-contractual obligations with cross-border elements under the Czech Act on Private International law, namely its regulation of international jurisdiction, applicable law, and the recognition and enforcement of foreign judgements.

A landscape architect preferences on border elements at green open spaces during Covid-19 pandemic

Green open space has many benefits for humans. Unfortunately, the function of green open space cannot be fully felt due to the Covid-19 pandemic. Even though the application of physical distancing in the garden can be created through the use of garden border elements. As a first step in determining good and effective elements, this study aims to explore the preferences of garden designers in selecting garden border elements, their shapes, and arrangements to maintain the distance between garden users. This research is a perceptual research using a survey method obtained through distributing questionnaires and processed by quantitative descriptive and chi-square test. As for the results obtained in this study, garden designers agree that the border element is an effective way that can be used to implement physical distancing in public parks. Although, in general, garden designers prefer hardscape over softscapes as a border element, there are different preferences for the type of hardscape and softscape used. Garden designers prefer fences, portable benches> 2m apart and concrete construction as a hardscape barrier that can be applied to gardens that have been built. Meanwhile, the selection of softscape in the garden that has been built tends to use flowering shrubs as a border element. The conclusion of this study is the preference for material selection that is different in terms of functionality and aesthetics is caused by the pandemic

A Comparative Look at the Divorce Systems in Egypt and Romania

Globalization, migration, and free movement have led to an increase in the diversity of international and inter-faith couples, and implicitly to an increase in the number of divorces with cross border elements. Although there are numerous regulations at European Union level, when it comes to international couples formed from an EU-citizen and a non-EU/third country one, for the purpose of this study Romanian, respectively Egyptian, there seems to be a lack of guidelines and cooperation mechanisms, ensuring the protection and enforcement of basic human rights. The main purposes of this study is examining in a comparative manner the divorce systems in Egypt and Romania and trying to answer the question of whether the differences are rooted in religion or are the result of different social, economic, cultural, and historical factors.

Developmental and Evaluation of Advanced Expression Vectors with Both Enhanced Integration and Stable Expression for Transgenic Farmed Fish

The objectives of the project were to develop expression vectors using the Sleeping Beauty transposon technology and the genetic border elements to provide both enhanced integration rate and stable transgene expression, and to evaluate the application of such vectors in farmed fish such as catfish and carp. The panel recommended adding the objective of evaluating the endogenous transposable elements, particularly in catfish, in order to evaluate the applicability of the expression vectors while reduc1ng efforts in real production of transgenic fish considering the focus of the project was to develop the vector and evaluation of its applicability, not producing transgenic fish. Efficient production of transgenic farmed fish is hindered by two major problems: mosaicism due to delayed integration after single-cell stage, and silencing of transgene expression. In this project, we proposed to combat these problems by coupling the Sleeping Beauty transposon technology that can enhance integration rate and the border elements that can insulate transgene from position effect. Our major objective was to develop a new generation of expression vector that contains both of these elements. We have developed expression vectors containing both the Sleeping Beauty transposon signals, inverted repeats and direct repeats (IR and DR, respectively), and the border elements, scs and scs'. Growth hormone minigene has been cloned into this vector for applications of such vectors in growth enhancement. Luc reporter gene has been also cloned into this vector cascades for relative easy evaluation of transgene expression. Transgenic fish have been produced using these expression vectors in both catfish (US) and carp (Israel). Much effort was also devoted to evaluation of the endogenous transposable elements in catfish as recommended by the BARD grant panel. Multiple families of Tcl-like transposons were identified from catfish. Surprisingly, many Tc I-related transcripts were identified. Among these transcripts, both the sense and antisense transcripts were present. Some of the transcripts may be useful for development of novel transposase-based technology for aquaculture applications in the future. This project has both scientific and aquaculture implications. First, to develop expression vectors containing both IR/DR and scs/scs' repeated elements have been reported being extremely technically difficult due to excision of the repeated sequences by the E. coli host during cloning processes. We have successfully constructed this advanced vector that contained very complex cascades for both gene integration and gene regulation. We have produced transgenic fish using such vectors. This advanced expression vector should be useful for production of transgenic fish. By simply replacing the growth hormone gene, any gene of interest can be readily inserted in this vector. Thus this vector should provide technological possibility for early integration and stable expression of any economically important genes in aquaculture. We have also evaluated the applications of the Sleeping Beauty-based vectors in terms of the impact of gene size and found that the size of trans gene drastically affects transposition. The system will be only useful for transferring genes smaller than 5.6 kb. We have also identified novel transposase-related transcripts that may be useful for the development of novel transposase-based technologies for general scientific research and for aquaculture applications.