Enhanced Thrombotic Responses Are Associated With Striatin Deficiency and Aldosterone

Background In addition to its role on blood pressure, aldosterone (ALDO) also affects the hemostatic system leading to increased experimental thrombosis. Striatin is an intermediate in the rapid, nongenomic actions of ALDO. Striatin heterozygote knockout ( Strn +/‐ ) mice have salt sensitivity of blood pressure and mildly chronically increased ALDO levels. In addition, in humans, striatin polymorphic gene variants are associated with increased salt sensitivity of blood pressure. Thus, we hypothesized that striatin deficiency would be associated with an increased prothrombotic response. Methods and Results Strn +/ ‐ mice and wild‐type littermates were maintained on a liberal sodium diet (1.6%). We measured in vivo thrombus formation following laser‐induced injury in cremaster arterioles using intravital microscopy. Mice were randomized to intravenous administration of ALDO or its vehicle. Acutely, ALDO increased thrombotic responses in wild‐type mice ( P <0.01) versus controls within minutes as determined by increased platelet accumulation and fibrin deposition at the site of laser injury. We then compared thrombus formation without ALDO administration in Strn +/‐ and wild‐type mice. Strn +/‐ mice showed highly significant increases in laser‐induced thrombosis ( P <0.001), as shown by increased platelet accumulation and fibrin deposition. Interestingly, the response in the Strn +/‐ mice basally was far greater than the wild‐type mice with ALDO administration, and ALDO administration produced no additional effect on thrombus responses in Strn +/‐ mice. Conclusions These results demonstrate a novel protective role of striatin in experimental thrombosis. Such a protective effect may be reduced in human striatin risk allele carriers, given the similar salt sensitivity of blood pressure in these individuals and Strn +/‐ mice.

The possibility of assessing the viability of the intestinal wall of rabbits in experimental mesenterial thrombosis with the help of the device «Minimax-Doppler-K»

Experimental thrombosis of mesenteric vessels was caused by the upper mesenteric artery compression by 30 minutes at different levels in 7 rabbits. With the help of microcirculation prarameters registered by the device «Minimax-Doppler-K» and ad oculus evaluation the zones of intestinal wall ischemia caused by thrombosis were determined. It is revealed that the ad oculus ischemia zones are significantly less common in area than the ischemia zones registered with the help of the device. Subsequent studies can reduce the % of insolvency of the seams after operations.