Aqueous Dried Extract of Skytanthus acutus Meyen as Corrosion Inhibitor of Carbon Steel in Neutral Chloride Solutions

The implementation of corrosion engineering control methods and techniques is crucial to extend the life of urban and industrial infrastructure assets and industrial equipment affected by natural corrosion. Then, the search of stable and environmentally friendly corrosion inhibitors is an important pending task. Here, we provide experimental evidence on the corrosion inhibitory activity of aqueous extracts of Skytanthus acutus Meyen leaf, a native plant from the Atacama Desert in northern Chile. Skytanthus extracts as a powder should be prepared at 55 °C to avoid thermal decomposition and loss of corrosion inhibitory activity. Corrosion of carbon steel AISI1020 immersed in 0.5 M NaCl was evaluated in the presence of different doses of Skytanthus extract by complementary and simultaneous linear polarization, electrochemical impedance spectroscopy, and weight-loss technique under high hydrodynamic conditions. Mixed Potential Theory was applied to confirm the electrochemical activity of the extract inhibitory capabilities. The Skytanthus extracts reached a 90% corrosion inhibitory efficiency when tested at 100 to 1200 ppm in a time span of 48 h, through an electrochemical interaction between the extract inhibitor component and the carbon steel surface. The corrosion inhibition activity observed in Skytanthus dry extracts involves a protective film formation by a mechanism that includes an iron dissolution at the expense of either oxygen reduction and/or hydrogen evolution, followed by a ferrous-ferric iron cycling, the formation of an iron complex and adsorption to the metal surface, and, finally, desorption or degradation of the protecting film. The water-soluble plant extract was subjected to HPLC-MS analyses that rendered 14 major signals, with quinic acid, protocatechuic acid, chlorogenic acid isomers, vanillic acid hexoside, and patuletin 3-methoxy-7-glucoside as the most abundant components. Then, we propose that a phenolic derivative is responsible for the corrosion inhibitory activity found in Skytanthus extracts.

Microbial processes during deposition and diagenesis of Banded Iron Formations

Banded Iron Formations (BIFs) are marine chemical sediments consisting of alternating iron (Fe)-rich and silica (Si)-rich bands which were deposited throughout much of the Precambrian era. BIFs represent important proxies for the geochemical composition of Precambrian seawater and provide evidence for early microbial life. Iron present in BIFs was likely precipitated in the form of Fe3+ (Fe(III)) minerals, such as ferrihydrite (Fe(OH)3), either through the metabolic activity of anoxygenic photoautotrophic Fe2+ (Fe(II))-oxidizing bacteria (photoferrotrophs), by microaerophilic bacteria, or by the oxidation of dissolved Fe(II) by O2 produced by early cyanobacteria. However, in addition to oxidized Fe-bearing minerals such as hematite (FeIII2O3), (partially) reduced minerals such as magnetite (FeIIFeIII2O4) and siderite (FeIICO3) are found in BIFs as well. The presence of reduced Fe in BIFs has been suggested to reflect the reduction of primary Fe(III) minerals by dissimilatory Fe(III)-reducing bacteria, or by metamorphic (high pressure and temperature) reactions occurring in presence of buried organic matter. Here, we present the current understanding of the role of Fe-metabolizing bacteria in the deposition of BIFs, as well as competing hypotheses that favor an abiotic model for BIF deposition. We also discuss the potential abiotic and microbial reduction of Fe(III) in BIFs after deposition. Further, we review the availability of essential nutrients (e.g. P and Ni) and their implications on early Earth biogeochemistry. Overall, the combined results of various ancient seawater analogue experiments aimed at assessing microbial iron cycling pathways, coupled with the analysis of the BIF rock record, point towards a strong biotic influence during BIF genesis.

Metagenomic Insights Into the Microbial Iron Cycle of Subseafloor Habitats

Microbial iron cycling influences the flux of major nutrients in the environment (e.g., through the adsorptive capacity of iron oxides) and includes biotically induced iron oxidation and reduction processes. The ecological extent of microbial iron cycling is not well understood, even with increased sequencing efforts, in part due to limitations in gene annotation pipelines and limitations in experimental studies linking phenotype to genotype. This is particularly true for the marine subseafloor, which remains undersampled, but represents the largest contiguous habitat on Earth. To address this limitation, we used FeGenie, a database and bioinformatics tool that identifies microbial iron cycling genes and enables the development of testable hypotheses on the biogeochemical cycling of iron. Herein, we survey the microbial iron cycle in diverse subseafloor habitats, including sediment-buried crustal aquifers, as well as surficial and deep sediments. We inferred the genetic potential for iron redox cycling in 32 of the 46 metagenomes included in our analysis, demonstrating the prevalence of these activities across underexplored subseafloor ecosystems. We show that while some processes (e.g., iron uptake and storage, siderophore transport potential, and iron gene regulation) are near-universal, others (e.g., iron reduction/oxidation, siderophore synthesis, and magnetosome formation) are dependent on local redox and nutrient status. Additionally, we detected niche-specific differences in strategies used for dissimilatory iron reduction, suggesting that geochemical constraints likely play an important role in dictating the dominant mechanisms for iron cycling. Overall, our survey advances the known distribution, magnitude, and potential ecological impact of microbe-mediated iron cycling and utilization in sub-benthic ecosystems.

Iron-fueled life in the continental subsurface: Deep Mine Microbial Observatory, SD, USA

Iron-bearing minerals are key components of the Earth’s crust and potentially critical energy sources for subsurface microbial life. The Deep Mine Microbial Observatory (DeMMO) is situated in a range of iron-rich lithologies, and fracture fluids here reach concentrations as high as 8.84 mg/L. Iron cycling is likely an important process given the high concentrations of iron in fracture fluids and detection of putative iron cycling taxa via marker gene surveys. However, a previous metagenomic survey detected no iron cycling potential at two DeMMO localities. Here, we revisit the potential for iron cycling at DeMMO using a new metagenomic dataset including all DeMMO sites and FeGenie, a new annotation pipeline that is optimized for the detection of iron cycling genes. We annotate functional genes from whole metagenomic assemblies and metagenome-assembled genomes and characterize putative iron cycling pathways and taxa in the context of local geochemical conditions and available metabolic energy estimated from thermodynamic models. We re-annotated previous metagenomic data, revealing iron cycling potential that was previously missed. Across both metagenomic datasets, we find that not only is there genetic potential for iron cycling at DeMMO, iron is likely an important source of energy across the system. In response to the dramatic differences we observed between annotation approaches, we recommend the use of optimized pipelines where the detection of iron cycling genes is a major goal. Importance We investigated iron cycling potential among microbial communities inhabiting iron-rich fracture fluids to a depth of 1.5km in the continental crust. A previous study found no iron cycling potential in the communities despite the iron-rich nature of the system. A new tool for detecting iron cycling genes was recently published, which we used on a new dataset. We combined this with a number of other approaches to get a holistic view of metabolic strategies across the communities, revealing iron cycling to be an important process here. In addition, we used the tool on the data from the previous study, revealing previously missed iron cycling potential. Iron is common in continental crust; thus, our findings are likely not unique to our study site. Our new view of important metabolic strategies underscores the importance of choosing optimized tools for detecting the potential for metabolisms like iron cycling that may otherwise be missed.