fungal adherence
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2021 ◽  
Vol 17 (6) ◽  
pp. e1009656
Author(s):  
Lijuan Mei ◽  
Xuewen Wang ◽  
Ying Yin ◽  
Guirong Tang ◽  
Chengshu Wang

The exopolysaccharide galactosaminogalactan (GAG) has been well characterized in Aspergilli, especially the human pathogen Aspergillus fumigatus. It has been found that a five-gene cluster is responsible for GAG biosynthesis in Aspergilli to mediate fungal adherence, biofilm formation, immunosuppression or induction of host immune defences. Herein, we report the presence of the conserved GAG biosynthetic gene cluster in the insect pathogenic fungus Metarhizium robertsii to mediate either similar or unique biological functions. Deletion of the gene cluster disabled fungal ability to produce GAG on germ tubes, mycelia and appressoria. Relative to the wild type strain, null mutant was impaired in topical infection but not injection of insect hosts. We found that GAG production by Metarhizium is partially acetylated and could mediate fungal adherence to hydrophobic insect cuticles, biofilm formation, and penetration of insect cuticles. In particular, it was first confirmed that this exopolymer is responsible for the formation of appressorium mucilage, the essential extracellular matrix formed along with the infection structure differentiation to mediate cell attachment and expression of cuticle degrading enzymes. In contrast to its production during A. fumigatus invasive growth, GAG is not produced on the Metarhizium cells harvested from insect hemocoels; however, the polymer can glue germ tubes into aggregates to form mycelium pellets in liquid culture. The results of this study unravel the biosynthesis and unique function of GAG in a fungal system apart from the aspergilli species.



2019 ◽  
Vol 209 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Bruna Rocha Almeida ◽  
Bianca Carla Silva Campitelli Barros ◽  
Ana Clara Liguori Araújo ◽  
Cristiane Alcantara ◽  
Erika Suzuki


2016 ◽  
Vol 40 (3-4) ◽  
pp. 727-742 ◽  
Author(s):  
Mei Gao ◽  
Hui Wang ◽  
LiJuan Zhu

Background: Vulvovaginal candidiasis (VVC) is a common gynecological disease. Candida albicans is believed to be mainly implicated in VVC occurrence, the biofilm of which is one of the virulence factors responsible for resistance to traditional antifungal agents especially to fluconazole (FCZ). Quercetin (QCT) is a dietary flavonoid and has been demonstrated to be antifungal against C. albicans biofilm. Methods: 17 C. albicans isolates including 15 clinical ones isolated from VVC patients were employed to investigate the effects of QCT and/or FCZ on the inhibition of C. albicans biofilm. Results: We observed that 64 µg/mL QCT and/or 128 µg/mL FCZ could (i) be synergistic against 10 FCZ-resistant planktonic and 17 biofilm cells of C. albicans, (ii) inhibit fungal adherence, cell surface hydrophobicity (CSH), flocculation, yeast-to-hypha transition, metabolism, thickness and dispersion of biofilms; (iii) down-regulate the expressions of ALS1, ALS3, HWP1, SUN41, UME6 and ECE1 and up-regulate the expressions of PDE2, NRG1 and HSP90, and we also found that (iv) the fungal burden was reduced in vaginal mucosa and the symptoms were alleviated in a murine VVC model after the treatments of 5 mg/kg QCT and/or 20 mg/kg FCZ. Conclusion: Together with these results, it could be demonstrated that QCT could be a favorable antifungal agent and a promising synergist with FCZ in the clinical management of VVC caused by C. albicans biofilm.



Author(s):  
Scott G. Filler ◽  
Donald C. Sheppard ◽  
John E. Edwards




2010 ◽  
Vol 249 (5) ◽  
pp. 693-700 ◽  
Author(s):  
Qingjun Zhou ◽  
Hao Chen ◽  
Mingli Qu ◽  
Qian Wang ◽  
Lingling Yang ◽  
...  


Mycoses ◽  
2010 ◽  
Vol 54 (5) ◽  
pp. e344-e351 ◽  
Author(s):  
C. A. Zamperini ◽  
A. L. Machado ◽  
C. E. Vergani ◽  
A. C. Pavarina ◽  
E. C. Rangel ◽  
...  
Keyword(s):  


2009 ◽  
Vol 47 (2) ◽  
pp. 185-196 ◽  
Author(s):  
Estela Ruiz-Baca ◽  
Conchita Toriello ◽  
Armando Pérez-Torres ◽  
Myrna Sabanero-López ◽  
Julio C. Villagómez-Castro ◽  
...  


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