Integration of Instruments for the Protection of Natural Protected Areas in Urban and Biodiversity Strategies and in Urban Planning Regulations

Approximately 75% of the urban settlements in Romania are superimposed or are tangent to at least one natural protected area, these not being integrated from the point of view of their regulation in the urban strategies and in the urban planning regulations. From a spatial point of view, this type of relationship often represents a contrast between the urban fabric and the quasi-natural fabric. However, in the regulatory or strategy instruments for the development of urban settlements, where such contrasts exist, they are only integrated at the border level. The ecotone is, in most cases, the only element mentioned in urban planning instruments and is approached as a land that can only function in isolation and that in no way can support urban development. This reluctance and fear of approaching natural protected areas, also negatively influences the conception of the community, investors and the administration. Urban actors are not informed and therefore not motivated, but neither do they have the opportunity to get involved in the conservation and protection process. The purpose of this article is to research urban and biodiversity strategies at E.U level, to identify gaps in the formulation of urban planning tools, what are the reasons behind generating these gaps and how they can be eliminated, or at least mitigated. The analysis will focus on some models of urban strategies which address natural protected areas, but will also consider related elements, directly related to their conservation, urban ecology and the involvement in the process of urban actors.

Spatial Point Pattern Analysis Identifies Mechanisms Shaping the Skin Parasite Landscape in Leishmania donovani Infection

Increasing evidence suggests that in hosts infected with parasites of the Leishmania donovani complex, transmission of infection to the sand fly vector is linked to parasite repositories in the host skin. However, a detailed understanding of the dispersal (the mechanism of spread) and dispersion (the observed state of spread) of these obligatory-intracellular parasites and their host phagocytes in the skin is lacking. Using endogenously fluorescent parasites as a proxy, we apply image analysis combined with spatial point pattern models borrowed from ecology to characterize dispersion of parasitized myeloid cells (including ManR+ and CD11c+ cells) and predict dispersal mechanisms in a previously described immunodeficient model of L. donovani infection. Our results suggest that after initial seeding of infection in the skin, heavily parasite-infected myeloid cells are found in patches that resemble innate granulomas. Spread of parasites from these initial patches subsequently occurs through infection of recruited myeloid cells, ultimately leading to self-propagating networks of patch clusters. This combination of imaging and ecological pattern analysis to identify mechanisms driving the skin parasite landscape offers new perspectives on myeloid cell behavior following parasitism by L. donovani and may also be applicable to elucidating the behavior of other intracellular tissue-resident pathogens and their host cells.