The Sustainable Treatment Effect of Constructed Wetland for the Aquaculture Effluents from Blunt Snout Bream (Megalobrama amblycephala) Farm

In aquaculture, constructed wetland (CW) has recently attracted attention for use in effluent purification due to its low running costs, high efficiency and convenient operation,. However, less data are available regarding the long-term efficiency of farm-scale CW for cleaning effluents from inland freshwater fish farms. This study investigated the effectiveness of CW for the removal of nutrients, organic matter, phytoplankton, heavy metals and microbial contaminants in effluents from a blunt snout bream (Megalobrama amblycephala) farm during 2013–2018. In the study, we built a farm-scale vertical subsurface flow CW which connected with a fish pond, and its performance was evaluated during the later stage of fish farming. The results show that CW improved the water quality of the fish culture substantially. This system was effective in the removal of nutrients, with a removal rate of 21.43–47.19% for total phosphorus (TP), 17.66–53.54% for total nitrogen (TN), 32.85–53.36% for NH4+-N, 33.01–53.28% NH3-N, 30.32–56.01% for NO3−-N and 42.75–63.85% for NO2−-N. Meanwhile, the chlorophyll a (Chla) concentration was significantly reduced when the farming water flowed through the CW, with a 49.69–62.01% reduction during 2013–2018. However, the CW system only had a modest effect on the chemical oxygen demand (COD) in the aquaculture effluents. Furthermore, concentrations of copper (Cu) and lead (Pb) were reduced by 39.85% and 55.91%, respectively. A microbial contaminants test showed that the counts of total coliform (TC) and fecal coliform (FC) were reduced by 55.93% and 48.35%, respectively. In addition, the fish in the CW-connected pond showed better growth performance than those in the control pond. These results indicate that CW can effectively reduce the loads of nutrients, phytoplankton, metals, and microbial contaminants in effluents, and improve the water quality of fish ponds. Therefore, the application of CW in intensive fish culture systems may provide an advantageous alternative for achieving environmental sustainability.

Photodegradation of Aquaculture Antibiotics Using Carbon Dots-TiO2 Nanocomposites

In this work, carbon dots (CD) were synthesized and coupled to titanium dioxide (TiO2) to improve the photodegradation of antibiotics in aquaculture effluents under solar irradiation. Oxolinic acid (OXA) and sulfadiazine (SDZ), which are widely used in aquaculture, were used as target antibiotics. To prepare nanocomposites of CD containing TiO2, two modes were used: in-situ (CD@TiO2) and ex-situ (CD/TiO2). For CD synthesis, citric acid and glycerol were used, while for TiO2 synthesis, titanium butoxide was the precursor. In ultrapure water (UW), CD@TiO2 and CD/TiO2 showed the largest photocatalytic effect for SDZ and OXA, respectively. Compared with their absence, the presence of CD@TiO2 increased the photodegradation of SDZ from 23 to 97% (after 4 h irradiation), whereas CD/TiO2 increased the OXA photodegradation from 22 to 59% (after 1 h irradiation). Meanwhile, in synthetic sea salts (SSS, 30‰, simulating marine aquaculture effluents), CD@TiO2 allowed for the reduction of SDZ’s half-life time (t1/2) from 14.5 ± 0.7 h (in absence of photocatalyst) to 0.38 ± 0.04 h. Concerning OXA in SSS, the t1/2 remained the same either in the absence of a photocatalyst or in the presence of CD/TiO2 (3.5 ± 0.3 h and 3.9 ± 0.4 h, respectively). Overall, this study provided novel perspectives on the use of eco-friendly CD-TiO2 nanocomposites for the removal of antibiotics from aquaculture effluents using solar radiation.

Biochar and Zeolite as Alternative Biofilter Media for Denitrification of Aquaculture Effluents

Denitrification processes are crucial in aquaculture as they convert the undesirable nitrate to safer forms of nitrogen. Conventionally, plastic media are used for the biofiltration of wastewater. However, alternative media may be as effective/better than plastic and enhance the sustainability of the system. This study evaluated biochar and zeolite as alternatives for the denitrification of aquaculture effluents. Triplicates of laboratory-scale bioreactors were fabricated to compare the denitrification efficiencies of biochar and zeolite to that of plastic. The bioreactors were fed synthetic aquaculture wastewater having nitrate loading rates of 50, 125, and 150 mg/L. Zeolite exhibited highest values of surface roughness in terms of arithmetic mean height (0.89 µm), maximum height (6.52 µm), and root-mean-square height (1.17 µm), as corroborated by surface profilometry and scanning electron microscopy. The results revealed that under pseudo-steady-state conditions, zeolite displayed the highest nitrate removal efficiency (maximum 95.02 ± 0.01%), which was followed by biochar and plastic (maximum 92.91 ± 0.01% and 92.57 ± 0.02%, respectively) due to its extraordinary surface roughness that provided better adhesion to the bacteria. However, by the end of the study, all the media exhibited comparable rates. Thus, both zeolite and biochar are sustainable alternatives of biomedia for nitrate removal. However, time and labor constraints must be accounted for to scale-up such bioreactors.