Dietary zinc concentration and lipopolysaccharide injection affect circulating trace minerals, acute phase protein response, and behavior as evaluated by an ear-tag based accelerometer in beef steers

To assess plasma trace mineral (TM) concentrations, the acute phase protein response, and behavior in response to a lipopolysaccharide (LPS) challenge, 96 Angus cross steers [average initial body weight (BW): 285 ± 14.4 kg] were sorted into two groups by BW (heavy and light; n = 48/group), fitted with an ear-tag based accelerometer (CowManager SensOor; Agis, Harmelen, Netherlands), and stagger started 14 d apart. Consecutive day BW were recorded to start the 24-d trial (d -1, 0). Dietary treatments began on d 0: common diet with either 30 (Zn30) or 100 (Zn100) mg supplemental Zn/kg DM (ZnSO4). On day 17 steers received one of the following injection treatments intravenously to complete the 2 × 3 factorial: 1) SALINE (~2-3 mL of physiological saline), 2) LOWLPS: 0.25 µg LPS/kg BW or 3) HIGHLPS: 0.375 µg LPS/kg BW. Blood, rectal temperature (RT), and BW were recorded on d 16 (-24 h relative to injection), and BW was used to assign injection treatment. Approximately 6, 24 (d 18), and 48 (d 19) h after treatment BW, RT, and blood were collected, and final BW recorded on d 24. Data were analyzed in Proc Mixed of SAS with fixed effects of diet, injection, diet × injection; for BW, RT, dry matter intake (DMI), plasma TM, and haptoglobin repeated measures analysis was used to evaluate effects over time. Area under the curve analysis determined by GraphPad Prism was used for analysis of accelerometer data. Body weight was unaffected by diet or injection (P ≥ 0.16), but there was an injection × time effect for DMI and RT (P < 0.05), where DMI decreased in both LPS treatments on d 16, but recovered by d 17, and RT was increased in LPS treatments 6 h post-injection. Steers receiving LPS spent less time highly active and eating than SALINE (P < 0.01). Steers in HIGHLPS spent lesser time ruminating, followed by LOWLPS and then SALINE (P < 0.001). An injection × time effect (P < 0.001) for plasma Zn showed decreased concentrations within 6 h of injection and remained decreased through 24 h before recovering by 48 h. A tendency for a diet × time effect (P = 0.06) on plasma Zn suggests plasma Zn repletion occurred at a greater rate in Zn100 compared to Zn30. These results suggest increased supplemental Zn may alter rate of recovery of Zn status from an acute inflammatory event. Additionally, ear-tag-based accelerometers used in this study were effective at detecting sickness behavior in feedlot steers, and rumination may be more sensitive than other variables.

397 Effect of Smartamine M on acute phase protein response in receiving beef heifers

Methionine supplementation can improve immune function in transition dairy cattle. Our objective was to determine if supplemental methionine could reduce acute phase protein responses in newly received growing cattle. 384 crossbred heifers (222 kg initial BW; southeastern U.S. origin) were received in 4 truckloads (blocks) over 9 d. BW was measured at arrival. The following day (d 0) cattle were vaccinated for viral and clostridial diseases, received Draxxin (2.5 mg tulathromycin/kg BW), and were stratified within block by arrival BW to 1 of 8 pens containing 12 animals each. Cattle (in 32 total pens) were limit-fed at 2.2% of BW daily (DM basis) a ration containing 40% Sweet Bran, 34.5% dry-rolled corn, 10% corn silage, 7.5% supplement, 4% alfalfa hay, and 4% prairie hay. Within blocks, pens were assigned to 1 of 2 treatments: 0 (control) or 10 g/d Smartamine M (SM) to provide 6 g/d metabolizable methionine. Pen weights were collected weekly to adjust feed offered the following week. Individual BW and tail-vein blood samples were collected on d 0, 14, and 45. Plasma haptoglobin was measured to assess acute phase protein response. Incidences of morbidity (1.6% for control, 2.6% for SM) and mortality (0.5% for both control and SM) were low. Between d 0 and 45, no differences were observed for ADG (1.24 vs. 1.27 kg/d; control vs. SM, P = 0.55) or G:F (0.107 vs. 0.110, P = 0.28), but DMI was 1.3% greater (P < 0.01) for control than SM due to differences in diet DM. An interaction between treatment and linear effect of day was detected for plasma haptoglobin (P < 0.05); over time, haptoglobin increased more for control (2.15, 2.28, and 2.95 mg/mL at 0, 14, and 45 d) than for SM (2.35, 2.37, and 2.58 mg/mL). Supplemental methionine may alleviate acute phase protein responses in stressed receiving cattle.