Micromotor-based localized electroporation and gene transfection of mammalian cells

Herein, we studied localized electroporation and gene transfection of mammalian cells using a metallodielectric hybrid micromotor that is magnetically and electrically powered. Much like nanochannel-based, local electroporation of single cells, the presented micromotor was expected to increase reversible electroporation yield, relative to standard electroporation, as only a small portion of the cell’s membrane (in contact with the micromotor) is affected. In contrast to methods in which the entire membrane of all cells within the sample are electroporated, the presented micromotor can perform, via magnetic steering, localized, spatially precise electroporation of the target cells that it traps and transports. In order to minimize nonselective electrical lysis of all cells within the chamber, resulting from extended exposure to an electrical field, magnetic propulsion was used to approach the immediate vicinity of the targeted cell, after which short-duration, electric-driven propulsion was activated to enable contact with the cell, followed by electroporation. In addition to local injection of fluorescent dye molecules, we demonstrated that the micromotor can enhance the introduction of plasmids into the suspension cells because of the dielectrophoretic accumulation of the plasmids in between the Janus particle and the attached cell prior to the electroporation step. Here, we chose a different strategy involving the simultaneous operation of many micromotors that are self-propelling, without external steering, and pair with cells in an autonomic manner. The locally electroporated suspension cells that are considered to be very difficult to transfect were shown to express the transfected gene, which is of significant importance for molecular biology research.

A 3D Dynamic Molecular Dynamics Simulation Study: Diffusion of Two Types of Sphere Janus Colloidal Particles

The research of Janus colloidal particles has attracted much attention in recent years because of its huge prospects and applications in materials, chemicals, medicine, catalysis, etc. In order to reduce the cost of conducting experiments, the method of molecular dynamics simulation is used to conduct research on Janus particles. However, the current related simulation study lacks the visualization, dynamization and three-dimensionalization of the Janus particle simulation process. Therefore, the author simulates the free diffusion process of two kinds of Janus particles by using 3D visualization dynamic molecular dynamics related software in this paper. By analyzing the influence of the related parameters of particles and droplet on the diffusion process, new directions of the researches about the simulation of Janus particles can be proposed. The simulation results show that the size of the droplet, the position where the droplet is added to the solution, and the mass of the particles will affect the diffusion process rate of the particles in the droplet in the surrounding solution.

Thermotaxis of Janus particles

The interactions of autonomous microswimmers play an important role for the formation of collective states of motile active matter. We study them in detail for the common microswimmer-design of two-faced Janus spheres with hemispheres made from different materials. Their chemical and physical surface properties may be tailored to fine-tune their mutual attractive, repulsive or aligning behavior. To investigate these effects systematically, we monitor the dynamics of a single gold-capped Janus particle in the external temperature field created by an optically heated metal nanoparticle. We quantify the orientation-dependent repulsion and alignment of the Janus particle and explain it in terms of a simple theoretical model for the induced thermoosmotic surface fluxes. The model reveals that the particle’s angular velocity is solely determined by the temperature profile on the equator between the Janus particle’s hemispheres and their phoretic mobility contrast. The distortion of the external temperature field by their heterogeneous heat conductivity is moreover shown to break the apparent symmetry of the problem. Graphic abstract