First record of mermithid larva (Nematoda: Mermithidae) in Anopheles maculipennis complex (Diptera: Culicidae) imago in Central-Europe

Between 2012 and 2014, in the sub-regions of Lake Balaton (Hungary) and the adjacent swamp called Kis-Balaton having stocks of biting mosquito species with large number of specimens, we regularly performed imago collections. In the pooled samples which were collected by aspirator and contained 6,906 adult insects, a nematode infected specimen was detected. The only worm in the abdomen of the female

PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania

The members of the Anopheles maculipennis complex have been incriminated for the transmission of the malaria in Europe, which was endemic until the middle of the century. The global warming and the intensification of the intercontinental travel constitute a risk of the re-emergence of the malaria in Europe, given the presence of the Anopheles vectors. The study has attempted the identification by using the PCR (Polymerase Chain Reaction) of the members of the Anopheles maculipennis complex from the North-eastern area of Romania from the city of Iaşi. In total there have been identified by using the PCR amplifying the ITS2 sequence of the ribosomal DNA, 217 specimens belonging to the complex of A. maculipennis among which: 58 A. atroparvus, 18 A. melanoon, 2 A. labranchiae, 52 A. maculipennis and 87 A. messeae. The ITS2 sequences of the ribosomal DNA have been compared to those of the species belonging to the A. maculipennis available in GenBank. The Species A. labranchiae is reported for the first time in Romania, being identified in the larval stage IV. The adaptation of a new species to the climatic conditions present in the North-eastern Romania, confirms the phenomenon of global warming and also the intensification of the travelling. As a result of the analysis of the A. labranchiae sequence, this one corresponds to the extent of 96% to the species from Italy, registered in GenBank, given the fact that a high number of the inhabitants of the municipality of Iaşi are working in this country.