PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania

2015 ◽  
Vol 60 (2) ◽  
Author(s):  
Maria-Larisa Ivanescu ◽  
Dumitru Acatrinei ◽  
Ionuţ Pavel ◽  
Tatiana Sulesco ◽  
Liviu Miron
Keyword(s):  
Global Warming ◽  
Ribosomal Dna ◽  
Its2 Sequence ◽  
Climatic Conditions ◽  
Eastern Area ◽  
The North ◽  
Anopheles Maculipennis Complex ◽  
Anopheles Maculipennis ◽  
North Eastern ◽  
Maculipennis Complex

AbstractThe members of the Anopheles maculipennis complex have been incriminated for the transmission of the malaria in Europe, which was endemic until the middle of the century. The global warming and the intensification of the intercontinental travel constitute a risk of the re-emergence of the malaria in Europe, given the presence of the Anopheles vectors. The study has attempted the identification by using the PCR (Polymerase Chain Reaction) of the members of the Anopheles maculipennis complex from the North-eastern area of Romania from the city of Iaşi. In total there have been identified by using the PCR amplifying the ITS2 sequence of the ribosomal DNA, 217 specimens belonging to the complex of A. maculipennis among which: 58 A. atroparvus, 18 A. melanoon, 2 A. labranchiae, 52 A. maculipennis and 87 A. messeae. The ITS2 sequences of the ribosomal DNA have been compared to those of the species belonging to the A. maculipennis available in GenBank. The Species A. labranchiae is reported for the first time in Romania, being identified in the larval stage IV. The adaptation of a new species to the climatic conditions present in the North-eastern Romania, confirms the phenomenon of global warming and also the intensification of the travelling. As a result of the analysis of the A. labranchiae sequence, this one corresponds to the extent of 96% to the species from Italy, registered in GenBank, given the fact that a high number of the inhabitants of the municipality of Iaşi are working in this country.

scholarly journals Distribution of Anopheles daciae and other Anopheles maculipennis complex species in Serbia

2018 ◽  
Vol 117 (10) ◽  
pp. 3277-3287 ◽  
Author(s):  
Mihaela Kavran ◽  
Marija Zgomba ◽  
Thomas Weitzel ◽  
Dusan Petric ◽  
Christina Manz ◽  
...  
Keyword(s):  
Complex Species ◽  
Anopheles Maculipennis Complex ◽  
Anopheles Maculipennis ◽  
Anopheles Daciae ◽  
Maculipennis Complex

Fan Lift in VTOL Design

1969 ◽  
Vol 73 (704) ◽  
pp. 657-664 ◽  
Author(s):  
R. G. West
Keyword(s):  
Traffic Control ◽  
Air Traffic Control ◽  
Air Traffic ◽  
Eastern Area ◽  
The Past ◽  
The North ◽  
Safe Level ◽  
Commercial Airline ◽  
Time Period ◽  
North Eastern

During the past two years the commercial airline operators and the travelling public alike have been forced to acknowledge the rapid increase in airport congestion. This problem is most marked in the North Eastern area of America at the present time but a similar situation will develop fairly soon at major airports in Europe. Even with the introduction of the airbus to scheduled services, the number of aircraft movements at conventional airports, within a given time period, will reach a maximum safe level. The restrictions are imposed partly by the large number of aircraft operations on each runway, and partly by Air Traffic Control safety limits.

The Anopheles maculipennis complex (Diptera: Culicidae) in Iran: molecular characterization and recognition of a new species

2003 ◽  
Vol 93 (6) ◽  
pp. 527-535 ◽  
Author(s):  
M.M. Sedaghat ◽  
Y.-M. Linton ◽  
M.A. Oshaghi ◽  
H. Vatandoost ◽  
R.E. Harbach
Keyword(s):  
New Species ◽  
Distinct Species ◽  
Nuclear Rdna ◽  
Pcr Assay ◽  
Dna Evidence ◽  
Anopheles Maculipennis Complex ◽  
Anopheles Maculipennis ◽  
Species Specific ◽  
A New Species ◽  
Maculipennis Complex

AbstractMosquitoes of the Anopheles maculipennis complex were collected in nine provinces of Iran (Esfahan, Fars, Gilan, Golestan, Kohkiluyeh va Boyerahmad, Mazandaran, Tehran, Azarbaijan-e Gharbi and Zanjan) between June 1983 and September 2002. The nuclear rDNA ITS2 sequences of 86 specimens were compared with those of seven species of the complex available in GenBank. Three genetically distinct species of the complex were distinguished: A. maculipennis Meigen, A. sacharovi Favre and a previously unrecognized species. The last species is most similar to, but clearly distinct from, A. martinius Shingarev and A. sacharovi. The taxonomy of A. martinius and A. sacharovi is critically reviewed, and justification is provided for formally recognizing the third species as Anopheles persiensissp.n. The new species is the first culicid to be characterized and named principally on the basis of DNA evidence. Anopheles persiensis was collected only in the northern Caspian Sea littoral provinces of Gilan and Mazandaran, and it seems likely that this species could be responsible for malaria transmission in this region that was previously attributed to A. maculipennis. A species-specific RFLP–PCR assay based on ITS2 sequences was developed to facilitate further studies of the three species in Iran.

Age and Growth of North Sea Echinoids

1985 ◽  
Vol 65 (3) ◽  
pp. 583-588 ◽  
Author(s):  
A. A. T. Sime ◽  
G. J. Cranmer
Keyword(s):  
Shallow Water ◽  
North Sea ◽  
Age And Growth ◽  
Eastern Area ◽  
The North ◽  
Small Variety ◽  
North Eastern ◽  
The North Sea ◽  
Northern North Sea

The genus Echinus is common throughout the entire northern North Sea. Echinus esculentus L. predominates in the shallow water off the eastern Scottish coast down to 100 m, while the small variety of Echinus acutus var. norvegicus (Düben and Koren) is rarely found in depths of less than 100 m and is most commonly located in the north-eastern area of the North Sea (Cranmer, 1985).

First record of mermithid larva (Nematoda: Mermithidae) in Anopheles maculipennis complex (Diptera: Culicidae) imago in Central-Europe

Biologia ◽  
2017 ◽  
Vol 72 (10) ◽  
Author(s):  
Marcell Sáringer-Kenyeres ◽  
Zoltán Kenyeres ◽  
Gábor Földvári ◽  
Gábor Majoros
Keyword(s):  
Central Europe ◽  
Mosquito Species ◽  
First Record ◽  
Lake Balaton ◽  
Anopheles Maculipennis Complex ◽  
Anopheles Maculipennis ◽  
Pooled Samples ◽  
Maculipennis Complex

AbstractBetween 2012 and 2014, in the sub-regions of Lake Balaton (Hungary) and the adjacent swamp called Kis-Balaton having stocks of biting mosquito species with large number of specimens, we regularly performed imago collections. In the pooled samples which were collected by aspirator and contained 6,906 adult insects, a nematode infected specimen was detected. The only worm in the abdomen of the female

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