Mapping Risks Associated with Soil Copper Contamination Using Availability and Bio-Availability Proxies at the European Scale

Soil contamination by trace elements like copper (Cu) can affect soil functioning. Environmental policies with guidelines and soil survey measurements still refer to the total contents of Cu in soils. However, Cu contents in soil solution or free Cu contents have been shown to be better proxies of risks of Cu mobility or (bio)availability for soil organisms. Several empirical equations have been defined at the local scale to predict the amounts of Cu in soil solution based on both total soil Cu contents and main soil parameters involved in the soil/solution partitioning. Nevertheless, despite the relevance for risk assessment, these equations are not applied at a large spatial scale due to difficulties to perform changes from local to regional. To progress in this challenge, we collected several empirical equations from literature and selected those allowing estimation of the amount of Cu in solution, used as a proxy of available Cu, from the knowledge of both total soil Cu contents and soil parameters. We did the same for the estimation of free Cu in solution, used as a proxy of bioavailable Cu These equations were used to provide European maps of (bio-)available Cu based on the one of total soil Cu over Europe. Results allowed comparing the maps of available and bio-available Cu at the European scale. This was done with respective median values of each form of Cu to identify specific areas of risks linked to these two proxies. Higher discrepancies were highlighted between the map of bioavailable Cu and the map of soil total Cu compared to the Cu available map. Such results can be used to assess environmental-related issues for land use planning.

Long-Term Effects of Forest Plantation Species on Chemical Soil Properties in Southern Rwanda

Understanding the long-term effects of tree species on soil properties is crucial for the development of forest restoration policies in relation to the choice of species that meet both environmental and local livelihood needs. This study was performed in the Arboretum of Ruhande, Southern Rwanda, where monocultures of 148 deciduous and 56 conifer species have been established in 0.25 ha replicated plots from 1933 onwards. We investigated the effects of six exotic and two native tree species planted in monoculture plots and native species mixed within one self-regenerated plot on soil properties in two layers (0–5 cm and 5–10 cm depth). We measured general soil properties (pH, SOM, exchangeable base cations) and water-soluble C and N as a proxy for soil functioning. Changes in soil properties were observed in the upper soil layer for all tree species. Planting Eucalyptus species caused soil acidification, whereas soil exchangeable cations and pH were higher under native species (Entandrophragma excelsum and Polyschias fulva) and mixed native species. The effects of tree species were more pronounced for hot water-extractable C and N than for other soil properties. Their analyses could be used for detecting changes in soil functioning linked to vegetation types.

Reflections and Insights on the Evolution of the Biological Remediation of Contaminated Soils

The field of soil biological remediation was initially focused on the use of microorganisms. For organic contaminants, biostimulation and bioaugmentation were the strategies of choice. For heavy metals, bioremediation was centered on the feasibility of using microorganisms to reduce metal toxicity. Partly due to the impossibility to degrade metals, phytoremediation emerged proposing the use of plants to extract them (phytoextraction) or reduce their bioavailability (phytostabilization). Later, microbial-assisted phytoremediation addressed the inoculation of plant growth-promoting microorganisms to improve phytoremediation efficiency. Similarly, plant-assisted bioremediation examined the stimulatory effect of plant growth on the microbial degradation of soil contaminants. The combination of plants and microorganisms is nowadays often recommended for mixed contaminated soils. Finally, phytomanagement emerged as a phytotechnology focused on the use of plants and associated microorganisms to decrease contaminant linkages, maximize ecosystem services, and provide economic revenues. Although biological remediation methods have been in use for decades, the truth is that they have not yet yielded the expected results. Here, we claim that much more research is needed to make the most of the many ways that microorganisms have evolutionary developed to access the contaminants and to better understand the soil microbial networks responsible, to a great extent, for soil functioning.

Effect of conservation agriculture practices on the resilience of Mediterranean soils to the predicted seasonal drought events

<p>How climate change will affect soil functioning is a major concern, especially in Mediterranean agrosystems, where, according to climate change projections, the occurrence of extreme temperatures and drought events will be increased. The main objective of our experiment was to evaluate the effect of land management (tillage system) on soil resilience against a simulated dry-rewetting cycle. Soil samples were collected from an in-situ field experiment established in 2008 in the Guadalquivir Valley, where conservation agriculture practices have been tested. Three different land management practices under a typical Mediterranean wheat-legume rotation system were compared: 1) traditional tillage (TT), 2) minimum tillage (MT) and 3) no-tillage (NT). Following our hypothesis, conservation agriculture practices (reduced tillage and no-tillage) may allow a more mature soil microbial community by reducing soil perturbation, and this would result in higher resistance of soil functioning against drought periods. Soil enzyme activities (β-glucosidase, phosphatase, acetylglucosaminidase, aminopeptidase, and dehydrogenase activities), microbial functional diversity (Microresp method), and soil DNA concentration (as an index of microbial biomass) were analyzed in a base-line sampling. Afterwards, a dry-rewetting cycle was simulated under controlled conditions. 8 subsamples of 50g from each soil sample were hydrated to reach 70% of each soil water holding capacity (WHC) and kept in those conditions for a pre-incubation period of 15 days. After this period, half of the replicates were let dry for 12 days (drought), while the others were maintained at 70% WFC (controls). Finally, all replicates were rehydrated again to the initial water content during a 14 days rewetting period. During this cycle, soil respiration rates were periodically measured to study the evolution of soil microbial activity. Our results showed that initial respiration rates were slightly higher in MT compared to NT (p<0.1), likely due to higher organic C and N content in the MT soils. Drought extremely reduced respiration rates in the three treatments, but the results did not show a clear pattern among treatments. During the rewetting period, respiration rates were significantly higher in drought samples in comparison with the controls, while no significant differences were found for the land management treatments. Besides, land management practices did not have a significant effect on soil DNA concentration, functional diversity of the microbial community, or enzyme activities. To conclude, the absence of a clear effect of land management practices on soil resilience to drought may be due to the experimental conditions. An in-situ experiment will allow us to determine if tillage reduction enhances soil resilience to moisture stress.</p>

Indicators of soil functioning in conventional, conservation and organic agriculture

<p>Sustainable management of agricultural systems is a major challenge for ensuring food security of the growing world population. Organic farming and reduced tillage are assumed to be sustainable agricultural practices improving soil quality relative to conventional management strategies. However, assessment of soil quality is often restrained to either physical, chemical or biological parameters. Soil organic carbon (SOC) is the most widely used indicator of soil quality, but it is not necessarily reactive to change, nor representative of the functioning of soil systems, in particular in relation to the realization of soil microbial processes, as it is composed of fractions with different availability for microbial activity and decomposition.</p><p>The objective of this study was to assess the influence of three major cropping systems: organic agriculture and conservation agriculture (no/reduced-tillage) vs. conventional agriculture on SOC fractions and microbial processes related to C and N cycling and to establish relationships between carbon fractions and microbial processes in order to identify the most relevant indicator of soil functioning. We hypothesized that 1) organic farming and conservation agriculture would improve soil functioning, 2) labile organic fractions would be better indicators of soil functioning.</p><p>We measured C and N in physical and chemical fractions (bulk, <20 µm, 20-2000µm, dissolved organic C, hot water extractable C, water soluble C, K<sub>2</sub>SO<sub>4</sub> extractable C, microbial biomass C) and microbial processes (respiration potential, net N mineralization, metabolic diversity of soil bacteria) in 16 cropland sites in Wallonia, south Belgium (CARBIOSOL project).</p><p>Preliminary data analyses indicate improved soil functioning in organic managed sites relative to sites under conventional and conservation management and reveal hot water extractable carbon as a promising proxy for monitoring changes in soil functioning in response to agricultural practices. Final detailed data analyses will be presented.</p>

Soil microbial composition and carbon mineralization are associated with vegetation type and temperature regime in mesocosms of a semiarid ecosystem

ABSTRACT Transition from historic grasslands to woody plants in semiarid regions has led to questions about impacts on soil functioning, where microorganisms play a primary role. Understanding the relationship between microbes, plant diversity and soil functioning is relevant to assess such impacts. We evaluate the effect that plant type change in semiarid ecosystems has for microbial diversity and composition, and how this is related to carbon mineralization (CMIN) as a proxy for soil functioning. We followed a mesocosm experiment during 2 years within the Biosphere 2 facility in Oracle, AZ, USA. Two temperature regimes were established with two types of plants (grass or mesquite). Soil samples were analyzed for physicochemical and functional parameters, as well as microbial community composition using 16S rRNA amplicon metagenomics (Illumina MiSeq). Our results show the combined role of plant type and temperature regime in CMIN, where CMIN in grass has lower values at elevated temperatures compared with the opposite trend in mesquite. We also found a strong correlation of microbial composition with plant type but not with temperature regime. Overall, we provide evidence of the major effect of plant type in the specific composition of microbial communities as a potential result of the shrub encroachment.