Navigation of a Freely Walking Fruit Fly in Infinite Space Using a Transparent Omnidirectional Locomotion Compensator (TOLC)

Animal behavior is an essential element in behavioral neuroscience study. However, most behavior studies in small animals such as fruit flies (Drosophila melanogaster) have been performed in a limited spatial chamber or by tethering the fly’s body on a fixture, which restricts its natural behavior. In this paper, we developed the Transparent Omnidirectional Locomotion Compensator (TOLC) for a freely walking fruit fly without tethering, which enables its navigation in infinite space. The TOLC maintains a position of a fruit fly by compensating its motion using the transparent sphere. The TOLC is capable of maintaining the position error < 1 mm for 90.3% of the time and the heading error < 5° for 80.2% of the time. The inverted imaging system with a transparent sphere secures the space for an additional experimental apparatus. Because the proposed TOLC allows us to observe a freely walking fly without physical tethering, there is no potential injury during the experiment. Thus, the TOLC will offer a unique opportunity to investigate longitudinal studies of a wide range of behavior in an unrestricted walking Drosophila.

Flight and Walking Performance of Dark Black Chafer Beetle Holotrichia parallela (Coleoptera: Scarabaeidae) in the Presence of Known Hosts and Attractive Nonhost Plants

Holotrichia parallela damages seriously on peanut (Arachis hypogaea) pods. Elucidation of its flight and walking performance in the presence of different plants may provide an insight in its host selection process and an explanation to its strong olfactory preference to an attractive nonhost, castor bean (Ricinus communis). We determined the relationships among flight performance, mate choice, and body weight of H. parallela beetles, and then investigated their flight and walking patterns in the presence of known hosts and attractive nonhost plants using a flight mill and a locomotion compensator, respectively. Body weights were not related to mating success, regardless of sex. The flight proportion of selected females drastically decreased compared with nonselected females, nonselected males, and selected males. Within mated males, heavier individuals exhibited poorer flight performance than lighter ones. In flight bioassay, peanut showed an arrestment effect on virgin females. For walking activity factors (distance, time, and speed), the host plants velvetleaf (Abutilon theophrasti) and Siberian elm (Ulmus pumila) elicited the strongest responses in females and males, respectively. Interestingly, the most preferred adult host, Siberian elm, and the nonhost, castor bean, elicited the highest values of two orientation factors (orientation and upwind length) in females. The chemical similarity hypothesis, which states that feeding or oviposition of insects mistakenly on nonhost can be traced to their chemical similarity to actual hosts, could explain the attraction of H. parallela to castor bean.

Shades of yellow: interactive effects of visual and odour cues in a pest beetle

Background:The visual ecology of pest insects is poorly studied compared to the role of odour cues in determining their behaviour. Furthermore, the combined effects of both odour and vision on insect orientation are frequently ignored, but could impact behavioural responses.Methods:A locomotion compensator was used to evaluate use of different visual stimuli by a major coleopteran pest of stored grains (Sitophilus zeamais), with and without the presence of host odours (known to be attractive to this species), in an open-loop setup.Results:Some visual stimuli—in particular, one shade of yellow, solid black and high-contrast black-against-white stimuli—elicited positive orientation behaviour from the beetles in the absence of odour stimuli. When host odours were also present, at 90° to the source of the visual stimulus, the beetles presented with yellow and vertical black-on-white grating patterns changed their walking course and typically adopted a path intermediate between the two stimuli. The beetles presented with a solid black-on-white target continued to orient more strongly towards the visual than the odour stimulus.Discussion:Visual stimuli can strongly influence orientation behaviour, even in species where use of visual cues is sometimes assumed to be unimportant, while the outcomes from exposure to multimodal stimuli are unpredictable and need to be determined under differing conditions. The importance of the two modalities of stimulus (visual and olfactory) in food location is likely to depend upon relative stimulus intensity and motivational state of the insect.

Shades of yellow: interactive effects of visual and odour cues in a pest beetle

Background. The visual ecology of pest insects is poorly studied compared to the role of odour cues in determining their behaviour. Furthermore, the combined effects of both odour and vision on insect orientation are frequently ignored, but could impact behavioural responses. Methods. A locomotion compensator was used to evaluate use of different visual stimuli by a major coleopteran pest of stored grains ( Sitophilus zeamais ), with and without the presence of host odours (known to be attractive to this species), in an open-loop setup. Results. Some visual stimuli – in particular, one shade of yellow, solid black and high contrast black-against-white stimuli – elicited positive orientation behaviour from the beetles in the absence of odour stimuli. When host odours were also present, at 90° to the source of the visual stimulus, the beetles presented with yellow and vertical black-on-white grating patterns changed their walking course and typically adopted a path intermediate between the two stimuli. The beetles presented with a solid black-on-white target continued to orient more strongly towards the visual than the odour stimulus. Discussion. Visual stimuli can strongly influence orientation behaviour, even in species where use of visual cues is sometimes assumed to be unimportant, while the outcomes from exposure to multimodal stimuli are unpredictable and need to be determined under differing conditions. The importance of the two modalities of stimulus (visual and olfactory) in food location is likely to depend upon relative stimulus intensity and motivational state of the insect.

Shades of yellow: interactive effects of visual and odour cues in a pest beetle

Background. The visual ecology of pest insects is poorly studied compared to the role of odour cues in determining their behaviour. Furthermore, the combined effects of both odour and vision on insect orientation are frequently ignored, but could impact behavioural responses. Methods. A locomotion compensator was used to evaluate use of different visual stimuli by a major coleopteran pest of stored grains ( Sitophilus zeamais ), with and without the presence of host odours (known to be attractive to this species), in an open-loop setup. Results. Some visual stimuli – in particular, one shade of yellow, solid black and high contrast black-against-white stimuli – elicited positive orientation behaviour from the beetles in the absence of odour stimuli. When host odours were also present, at 90° to the source of the visual stimulus, the beetles presented with yellow and vertical black-on-white grating patterns changed their walking course and typically adopted a path intermediate between the two stimuli. The beetles presented with a solid black-on-white target continued to orient more strongly towards the visual than the odour stimulus. Discussion. Visual stimuli can strongly influence orientation behaviour, even in species where use of visual cues is sometimes assumed to be unimportant, while the outcomes from exposure to multimodal stimuli are unpredictable and need to be determined under differing conditions. The importance of the two modalities of stimulus (visual and olfactory) in food location is likely to depend upon relative stimulus intensity and motivational state of the insect.

A Visually Induced Switch in Mode of Locomotion of a Spider

The spider Cupiennius salei Keys. (Araneae, Ctenidae) is a nocturnal hunter living on monocotyledones. It does not build webs for prey capture. During the day it remains in its retreat and during dusk it begins to hunt for prey or to search for mates. C. salei is well equipped with mechanosensory systems to detect air- or substrate- borne vibrations elicited by prey or predators. If none of them produce either air movements or substrate vibrations, and the light intensity is below the threshold (0.1 lx), the animal is virtually " blind" . There­ fore a hypothetical, additional sensory input should exist, which is used only in complete darkness. The animal was tested on a locomotion compensator were it performs constant walks towards a visual target. Three different light intensities were used (bright 200 lx, dim 0.1 lx, and dark at 950 nm, which is outside the spectral sensitivity range of the animal). At bright and dim illumination the animal walked in the alternating tetrapod gait towards the target. In complete " darkness" the walk was no longer directed and the animal changed its gait and continued walking on only six legs using the first pair as guide-sticks. If the first pair is missing, the second cannot replace this function. This shows a twofold use of the first pair o f legs as ordinary walking legs and as guide-sticks or " antennae" . Therefore one can assume that visual input causes a behavioral change, which can not be explained by a fixed locomotion pattern but by adaptive changes caused by visual inputs.