PSIII-1 Influence of prenatal or postnatal exposure to gossypol from cottonseed meal on semen quality in commercial boars: a preliminary study on feral hog control

The objective was to examine the influence of prenatal (Experiment 1) or postnatal (Experiment 2) exposure to gossypol from cottonseed meal (CSM) on semen quality in commercial boars. In Experiment 1, pregnant sows (n = 5) were fed a diet containing 0% (n = 1), 0.04% (n = 2), or 0.08% (n = 2) gossypol between d 56 and 86 of gestation. Boars (n = 11) born to sows in each treatment group (0% gossypol n = 3; 0.04% gossypol n = 4; 0.08% gossypol n = 4) were fed a common diet without CSM, and semen was collected at 269±2 d of age using a live sow in estrus. In Experiment 2, boars (n = 21) were fed a diet containing 0%, 0.02%, or 0.04% gossypol between 63±1 and 105±1 d of age (Initial BW: 19.85±0.43 kg). After the treatment period, boars were fed a common diet without CSM, and semen was collected at 238±7 d of age using a breeding dummy. Sperm cell concentration, percentage of motile sperm cells, and percentage of progressively motile sperm cells were analyzed using the MIXED procedure of SAS with treatment as a fixed effect in Experiment 1 and 2 and dam as a random effect in Experiment 1. In Experiment 2, semen was not successfully collected from every boar; therefore, chi-square analysis was used to assess semen collection status between treatment groups using the FREQ procedure of SAS. In Experiment 1, there was no difference in sperm concentration (P = 0.45), percent motility (P = 0.71), or percent progressive motility (P = 0.27) between treatment groups. In Experiment 2, there was no difference in sperm concentration (P = 0.72), percent motility (P = 0.17), or percent progressive motility (P = 0.87) between treatment groups. No difference was observed in boar collection status between treatment groups (P = 0.77). In conclusion, prenatal or postnatal exposure to gossypol from CSM did not influence semen quality in commercial boars.

135 Effect of Gossypol from Cottonseed Meal on Growth Performance, Plasma Gossypol, and Complete Blood Cell Counts in Commercial Growing Pigs: A Preliminary Study on Feral Hog Control

To evaluate the effect of cottonseed meal (CSM) on growth performance, plasma gossypol and complete blood cell (CBC) counts in growing pigs, 40 gilts (Exp 1) and 24 boars (Exp 2), 63 day of age (19.85±0.43 kg), were randomly allotted to 1 of 4 and 3 treatments with 2 replicates/treatments, respectively. Treatments for Exp 1 during phase 1–3 (14 d/phase) were a nutrient adequate control diet (NRC, 2012) without CSM (0% gossypol), and increasing levels of CSM was added to produce diets containing 0.01%, 0.02% and 0.04% gossypol to form treatments 2 to 4, respectively. For Exp 2, treatments were the same as those in the gilt trail, except 0.01% gossypol treatment was eliminated. All pigs were fed a common diet without CSM in phase 4 (14 d). Whole blood was obtained from two close-to-average pen-BW pigs repeatedly at each phase to determine CBC in Exp 1 and plasma gossypol in Exp 1 and 2. Data were analyzed using the Mixed procedures of SAS (Cary, NC). ADG did not significantly differ between treatments in phase 1&2 (P > 0.05). In phase 3, ADG decreased linearly and quadratic (P < 0.05) with increasing level of CSM in gilts and boars, respectively, while ADFI did not differ. Neutrophil concentration was higher while mean corpuscular volume (MCV) was lower in gilts fed CSM on d 42 than those fed control regardless level of inclusion, whereas after 14 d of CSM withdrawal, neutrophil level was similar to control and MCV remained low (Treatment*day, P < 0.01). Plasma gossypol increased with increasing level of CSM in both gilts and boars during phase 1–3, and was still higher than control after pigs were fed a common diet for 14 d (P < 0.05). In conclusion, cottonseed meal derived gossypol impairs growth performance, and increase plasma gossypol in gilts and boars.