Evaluation of the aesthetic qualities and adaptability of introduced ornamental apple trees from the genetic collection of the Orenburg Branch of the Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery

The study of ornamental and adaptive qualities of Malus Mill. from the genetic collection of ornamental crops of the Orenburg branch of the Research Center of Horticulture was carried out to assess the gene pool of introduced wild apple tree species and forms and identify suitable for the urban landscaping and the breeding process. Such characteristics as habitus, abundance of flowering, color and number of flowers in the inflorescence, color of vegetative organs, flowers and fruits during the season were taken into account; resistance to major diseases and adverse abiotic factors was evaluated. Additionally, the color of buds and flowers was characterized on the basis of the RHS (Royal Horticultural Society Color Chart). As a result of a preliminary survey on the complex of ornamental qualities and the level of adaptability, 5 most promising species were identified, which received the best ratings on the ornamental scale. Malus sikkimensis (Wenz.) Koehne ex C. K. Schneid. (31 points) was characterized by an attractive habit, the presence of flowers with a diameter of up to 40 mm and subsequently fruits that acquire a yellow-red color. Malus sargentii Rehder (29 points) was notable for its compact rounded crown, a large number of flowers with a diameter of 30 mm, followed by the formation of fruits with a dark red color. Malus floribunda Siebold ex Van Houtte (28 points) was distinguished by a large number of fragrant flowers in an umbrella-shaped inflorescence (5 — 7 pcs) with an average diameter of 30 mm, as well as attractive red-yellow fruits. Malus sieboldii (Regel) Rehder, M. toringo Siebold (28 points) was interesting primarily by the presence of 5 -7 flowers of 20 — 30 mm in the inflorescence and decorative fruits, the color of which varies from yellow to red. Malus niedzwetzkyana Dieck (28 points) attracted attention to the rich purple color of all organs due to the presence of a large number of anthocyanins, it was also worth noting the presence of a pleasant aroma during flowering and large flowers with an average diameter of 40 mm. The selected ornamental types of apple trees are favorable for use in landscaping of various scales, they are recommended for breeding work as initial parent forms.

Understanding Alstroemeria pallida Flower Colour: Links between Phenotype, Anthocyanins and Gene Expression

Flower colour is mainly due to the accumulation of flavonoids, carotenoids and betalains in the petals. Of these pigments, flavonoids are responsible for a wide variety of colours ranging from pale yellow (flavones, flavonols and flavanodiols) to blue-violet (anthocyanins). This character plays a crucial ecological role by attracting and guiding pollinators. Moreover, in the ornamental plants market, colour has been consistently identified as the main feature chosen by consumers when buying flowers. Considering the importance of this character, the aim of this study was to evaluate flower colour in the native Chilean geophyte Alstroemeria pallida, by using three different approaches. Firstly, the phenotype was assessed using both a colour chart and a colourimeter, obtaining CIELab parameters. Secondly, the anthocyanin content of the pigmented tepals was evaluated by high-performance liquid chromatography (HPLC), and finally, the expression of two key flavonoid genes, chalcone synthase (CHS) and anthocyanidin synthase (ANS) was analysed using real-time polymerase chain reaction (PCR). Visual evaluation of A. pallida flower colour identified 5 accessions, ranging from white (Royal Horticultural Society (RHS) N999D) to pink (RHS 68C). Moreover, this visual evaluation of the accessions correlated highly with the CIELab parameters obtained by colourimetry. An anthocyanidin corresponding to a putative 6-hydroxycyanidin was identified, which was least abundant in the white accession (RHS N999D). Although CHS was not expressed differentially between the accessions, the expression of ANS was significantly higher in the accession with pink flowers (RHS 68C). These results suggest a correlation between phenotype, anthocyanin content and ANS expression for determining flower colour of A. pallida, which could be of interest for further studies, especially those related to the breeding of this species with ornamental value.

Galeopsis speciosa (Lamiaceae): an Open Vegetation seed bank community at Worsley in Salford (v.c.59), revealed during construction of the new Royal Horticultural Society Garden at Bridgewater

This paper provides a baseline flora for the site of the new garden of the Royal Horticultural Society at Worsley New Hall in Salford (v.c.59). During construction, 35,000 m3 of top-soil, sub-soil and spoil were stripped and stored onsite; species recruiting from these seed banks were monitored 2017-2020, leading to the description of a new Galeopsis speciosa Open Vegetation plant community. Four commercial wildflower mixes were used during post-construction landscaping in 2019, and their establishment was assessed in 2020. It will be interesting to follow the survival of these introduced species, many of which are not native to the site.

Measuring Camellia Petal Color Using a Portable Color Sensor

The color of petals of flowering plants is often determined by comparing one or more of the petals to various Royal Horticultural Society (RHS) Colour Chart cards until a color match is found. However, these cards are susceptible to fading with age and can also provide inaccurate results if lighting is not optimal. The cards also rely on the human eye to determine a match, which introduces the possibility of human error. The objectives of this study were to determine camellia (Camellia japonica L.) petal color using the RHS Colour Chart, to determine camellia petal color with the NixTM Pro color sensor (Nix Sensor Ltd., Hamilton, Ontario, Canada), and to compare these measurements using different color measuring approaches. Color measurements of camellia flower petals using the NixTM Pro color sensor were compared to published CIELAB values from the Royal Horticultural Society (RHS) Colour Chart. Forty-five petal color samples were collected from fifteen different camellia shrubs. The RHS Colour Chart was used for each of the petals, and the RHS identifications were recorded. Measurements using the NixTM Pro color sensor were compared to RHS-provided CIELAB values that corresponded with the recorded identification for each petal to determine accuracy. The NixTM Pro color sensor’s measurements were also compared to a mean of the values, multiple measurements on the same petal location, and multiple measurements on different petal locations to determine precision and variation. The Nix™ Pro color sensor’s readings were precise in petal color determination and provided more nuanced differences between petals of the same plant and plants of the same variety in each of the color categories. The RHS Colour Chart provided an accurate depiction of most petals, but it was difficult to use with petals that had wide color variation over the entire petal. The Nix™ Pro color sensor’s measurements appeared to have more variation in the b* color space. However, overall, the Nix™ Pro color sensor L*, a*, and b* values were highly correlated with the provided RHS values (p < 0.01), showing that the sensor can be used as an accurate and precise substitute for the RHS Colour Chart. The Nix™ Pro color sensor can be a useful, cost-effective tool to measure the petal color of camellia and other flowering plants and rectifies many of the problems associated with the RHS Colour Chart.

Student Project Environmental influences on box blight epidemics

Calonectria pseudonaviculata and C. henricotiae are two recently differentiated fungal species responsible for box blight, a disease that threatens the Buxus genus. Infection can be introduced to gardens on new plants and is spread through the use of tools. The fungus survives on stem lesions and fallen leaves when spores are dispersed by rainsplash. In this study, 195 Calonectria UK isolates collected by the Royal Horticultural Society (RHS) Advisory Service were identified to species level. Detached stem assays were performed to assess how long stem and leaf lesions remain infectious, and their sensitivity to fungicides. A survey was also carried out at three National Trust properties on the effect of clipping on box blight distribution and severity. It was found that C. henricotiae was only present in and after 2011. C. henricotiae is more thermotolerant, and the increase in prevalence may be a result of increasing temperature and longer dry spells in the UK. Sporulation could occur multiple times on both stem and leaf lesions in humid conditions, although spore production dropped markedly after six sporulation events. Fungicides were effective at preventing spore production on stem lesions. Long dry spells may also reduce Calonectria’s ability to sporulate, leading to limited box blight spread between plants.

Flavonoid Components of Different Color Magnolia Flowers and Their Relationship to Cultivar Selections

Magnolia (Magnoliaceae) is widely cultivated for its beauty; however, despite this, the components of the different flower colors in Magnolia have not been elucidated. In this study, the color parameters of 10 Magnolia petals with different colors were measured by the Royal Horticultural Society Color Chart (RHSCC) and a color reader CR-10. The composition and content of the flavonoids in the petals were analyzed by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) as well as HPLC with electrospray ionization and mass spectrometry (HPLC-ESI-MS2). All results showed that the 10 petals were divided into four color groups. Regarding the flavonoid composition, four types of anthocyanins, including Cyanidin-glucosyl-rhamnoside (Cy-GR), Cyanidin-glucosyl-rhamnosyl-glucoside (Cy-GRG), Peonidin-glucosyl-rhamnoside (Pn-GR), and Peonidin-glucosyl-rhamnosyl-glucoside (Pn-GRG), were identified, as well as 10 types of flavonols. The flavonols included isorhamnetin, quercetin, kaempferol, and their glycosides, which included rutinoside, rhamnose, and glucoside. Cyanidin and peonidin make Magnolia petals appear red-purple and purple, respectively, and the flavonols perform as evident auxiliary pigments, particularly quercetin. The Magnolia cultivar flower phenotypes sampled in this study differed by changes in their existing flavonoid content rather than by the appearance of new flavonoids. Consequently, this study provides a reference for further revealing the basis of Magnolia flower color and provides clues for color breeding.