SEMA6D, Negatively Regulated by miR-7, Contributing to Chondrocyte Catabolic and Anabolic Activities via p38 Signaling Pathway

Background: MiR-7 has been recognized as a promoting factor of osteoarthritis (OA), but the specific down-stream pathway of miR-7 still remains unknown. Further investigation of the molecular regulatory mechanism of miR-7 might help develop a novel therapeutic method for OA.Results: Here we revealed that Semaphorin 6D (SEMA6D) was a direct target gene of miR-7, of which presented a negatively regulatory relation in vitro and in vivo. Lucubration of SEMA6D suggested that SEMA6D is validated to promote the anabolic metabolism and reduce the catabolism of chondrocytes via inhibiting the activation of p38 pathway.Conclusions: Present research illustrated that SEMA6D is a negatively regulatory factor of miR-7 and a pivotal mediator of the catabolism and anabolism of chondrocytes. SEMA6D exerts its function via inhibiting the activation of p38 pathway.

Overexpression of LncRNA-PVT1 promotes proliferation migration, invasion and fibrosis in diabetic nephropathy via suppressing miR-93-5p

Background : This research aimed to explore the molecular mechanism of LncRNA- plasmacytoma variant translocation 1 (PVT1) in diabetic nephropathy (DN). Methods: Mouse mesangial cells (MMCs) were obtained from diabetic model mice. The real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), Western blot, luciferase reporter gene assay, EdU assay, flow cytometry analysis, AnnexinV-PI double staining, scratch assay, Transwell assay, enzyme-linked immunosorbent assay (ELISA) and luciferase reporter gene assay were performed to reveal the effect of PVT1 on the proliferation, migration, invasion and fibrosis of MMCs cultured in high glucose. Results: PVT1 was overexpressed in both mice kidney tissue and high glucose cultured MMCs. Meanwhile, PVT1 could not only promoted proliferation, migration, invasion and fibrosis of high glucose cultured MMCs, but also regulated the cell cycle from G0/G1 to S phase. Moreover, PVT1 negatively regulated the expression of miR-93-5p in MMCs. Furthermore, the PVT1-miR-93-5p regulatory relation activated the PI3K/Akt/mTOR pathway, which in turn regulated cell proliferation and insulin signaling in high glucose cultured MMCs. Conclusions: PVT1 might promoted proliferation, migration, invasion and fibrosis in high glucose cultured MMCs, which further affected the development of DN. Furthermore, PVT1-miR-93-5p regulatory relation might take part in DN progression via activating the PI3K/Akt/mTOR pathway.

WYNAGRODZENIE DOZORCY RZECZY RUCHOMYCH W POSTĘPOWANIU EGZEKUCYJNYM

THE CARETAKER’S FEE FOR THE CUSTODY OF MOVABLES IN FORECLOSURESummaryIn compliance with the relevant Polish provisions, movables impounded by a bailiff are to be left in the custody of the person with whom they were found. In Article 855 of the Polish Code of Civil Procedure the legislator recognises the bailiff’s option to put impounded movables in the custody of another person, includinga creditor, who then performs the duties of caretaker of the impoundedmovables. The aim of this article is to present issues related to thecaretaker’s fee in foreclosure proceedings. When a movable item isentrusted to the custody of a third party a regulatory relation in publiclaw arises between the bailiff and the caretaker. The caretaker’s fee ispart of the costs of repossession, and are payable in the foreclosureproceedings. The costs of repossession, including the caretaker’s fee, may not be claimed in separate proceedings.