Plant Growth Response ofPinus patulaandP. maximinoiSeedlings at Nursery to Three Types of Ectomycorrhizal Inocula

The objective of this study was to assess the response in seedling growth, root colonization, and P content of seedlings ofPinus maximinoiandP. patulato the inoculation with three types of ectomycorrhizal inocula with three doses (17.5, 35, and 70 kg·m−3) in nursery. The first inoculum was soil from aPinusplantations that contained three ectomycorrhizal fungi (Amanita muscaria, Amanitasp.,andSuillus luteus); the second was a crude inoculum composed by root fragments ofPinusseedlings colonized byS. luteussuspended in a sterile matrix soil-sand; the third inoculum was a mixture of two ectomycorrhizal fungiA. muscariaandS. luteusproduced underin vitroconditions in the potato-dextrose-agar medium. The results showed that the inoculum producedin vitrowas most effective to promote plant growth and ectomycorrhizal colonization of roots in both plant species. Also, the effects on seedlings were significantly higher with the increase of the doses. InP. patulathere were not significant effects on foliar P content with type and dose of inocula, whereas inP. maximinoithere were interactive effects of both factors. In this case, better results were obtained with the inoculum produced underin vitroconditions and with the highest dose.

The Effect of Scleroderma columnare Inoculum Doses to Enhance Ectomycorrhizal Colonization and Growth of Shorea javanica Seedling

Ectomycorrhiza helped plants to absorb nutrients and water. Shorea javanica belong to Dipterocarpaceae family and highly dependent on ectomycorrhiza to growth. Spore inoculation was one way to inoculate ectomycorrhiza fungi. This study aimed to get the best doses of spore Scleroderma columnare on colonization and enhancing growth of Shorea javanica seedling. This experiment used randomized complete design with 5 treatments and 3 replicates. The treatments were 0, 5, 10, 15, and 20 ml/polybag spore inoculum of S. columnare. Data obtained were analyzed by analysis of variance (anova) and continued with Least Significant Different (LSD). The results showed that added of 10 ml (6,5 x 107) gained higher root colonizatition (%). Dosis of 10 ml (6,5 x 107) and 20 ml (1,3 x 108) spore inoculum were able to improve plant growth on the parameters such as plant height, shoot dry weight, total dry weight, and total leaf area. Keywords: Dose of Inoculum, ectomycorrhiza, Scleroderma columnare, Shorea javanica.

Penggunaan Berbagai Media Tanam dan Inokulasi Spora Untuk Meningkatkan Kolonisasi Ektomikoriza dan Pertumbuhan Shorea javanica

Media is a decisive factor to succed the ectomycorrhizal colonization and plant growth. This study aimed to get the best planting media to increase ectomycorrhizal colonization and growth of Shorea javanica. The experiment was conducted in a greenhouse for four months. The experimental designed by randomized complete design with four treatments upon the planting media: soil, sand, husk and cocopeat. Ectomycorrhiza given as spore suspension (Scleroderma columnare) of 20 ml each plant. Data were analyzed using the Bartlett test to determine homogeneity of variance followed by analysis of variance and LSD. The results shown that the sand provides the best results compared to another media to form ectomycorrhiza colonization; while sand and soil were the best media to support the growth of S. javanica with inoculation of S. Columnare. Keyword : colonization, ectomycorrhiza, Shorea javanica, planting media