IDENTIFIKASI JENIS-JENIS PANDANACEAE DI PULAU PEPAYA PADA KAWASAN TAMAN NASIONAL TELUK CENDERAWASIH (Identification of Pandanaceae Types in Pepaya Island in The National Park Areas of Teluk Cenderawasih)

Diversitas pandanus di Papua masih banyak yang belum diketahui, walaupun cukup banyak yang sudah diidentifikasikan. Pandanus pada kawasan Taman Nasional Teluk Cenderawasih, sampai saat ini belum didokumentasikan dengan baik, sehingga diversitas pada pulau dan pesisirnya perlu diidentifikasikan kembali. Metode yang digunakan adalah metode deskriptif dengan teknik survei lapang dan wawancara semi struktural serta studi literatur. Variabel yang diamati dalam penelitian ini adalah jenis-jenis buah pandan, yang tampak saat ditemukan atau pada Herbarium Pusat Keanekaragaman Hayati (PPKH) Universitas Papua Manokwari dengan obyek utama yaitu jenis-jenis Pandanus sp. di pulau Pepaya pada kawasan Taman Nasional Teluk Cenderawasih. Jenis-jenis pandanus di Pulau Pepaya pada kawasan Taman Nasional Teluk Cenderawasih ditemukan sebanyak 3 jenis, yaitu: Pandanus tectorius, Pandanus odoratissimus, dan Pandanus polycephalus (yang berbeda).

In Vitro Assessment of Antioxidant, Thrombolytic, Antimicrobial Activities of Medicinal Plant Pandanus odoratissimus L. Leaves Extract

The present study was carried out to investigate phytochemical, antioxidant; antimicrobial, thrombolytic activity and estimate total phenolic, total flavonoid content of Pandanus odoratissimus (p.odoratissimus) leaves of methanol extract. In thrombolytic activity, aqueous soluble fraction (AQSF) exhibited highest percentage (46.58 %) of potential to lyse blood clot compared to standard drug streptokinase (69.52 %). In antimicrobial assay, dichloromethane soluble fraction (DCMSF) explored the highest diameter of clear zone of inhibition against both gram positive (19.60 ± 0.12 mm) and gram negative (20.00 ± 0.20 mm) bacteria compared to standard antibiotic, Kanamycin (50.00 ± 0.19). Levels of antioxidant were determined by DPPH assay followed by calculated IC50 values of different Kupchan extracts. The methyl soluble fraction (MSF) showed the lowest level of IC50 value (36.70 ± 0.32 µg/mL) in comparison to ascorbic acid (12.48 ± 0.09 µg/mL) while MSF disclosed the maximum level (62.19 ± 0.26 mg of GAE/g of extract) of total phenolic content in the extracts of P. odoratissimus. This study was conducted to validate the P. odoratissimus leaves used as a folk medicine such as, antioxidant, thrombolytic, and antimicrobial potential.

IN VITRO ANTIFUNGAL ACTIVITY OF FLOWER EXTRACT OF PANDANUS ODORATISSIMUS AGAINST DERMATOPHYTIC FUNGI

Objective: The present study aimed to evaluate the antifungal activity of Pandanus odoratissimus oil against dermatophytic fungi, and it was compared against the two commonly used antifungal agent’s fluconazole and griseofulvin.Methods: A total of seven strains of dermatophytes were tested for antifungal activity using oil extracted from the flower of P. odoratissimus by using agar-well diffusion method and the zone of inhibition was compared with antifungal agent’s fluconazole and griseofulvin. Minimum inhibitory concentration (MIC) was determined using the tube-dilution method.Results and Conclusion: The zone of inhibition varied from 16.32 to 19.76 mm for fluconazole, 12.12–18.16 mm for griseofulvin, and 2.5–9.59 mm and 7.63–12.88 mm for 2.5 mg/ml and 5 mg/ml of P. odoratissimus extract, respectively. Epidermophyton floccosum and Trichophyton violaceum showed the lowest MIC value of 0.15 mg/ml. The results of our study have shown that the extract from P. odoratissimus can work significantly better against fungal diseases caused by dermatophytes. It was also found that it acts as a perfect alternative to the currently available antifungals such as fluconazole and griseofulvin.