Effects of Elevated Carbon Dioxide and Chronic Warming on Nitrogen (N)-Uptake Rate, -Assimilation, and -Concentration of Wheat

The concentration of nitrogen (N) in vegetative tissues is largely dependent on the balance among growth, root N uptake, and N assimilation. Elevated CO2 (eCO2) plus warming is likely to affect the vegetative-tissue N and protein concentration of wheat by altering N metabolism, but this is poorly understood. To investigate this, spring wheat (Triticum aestivum) was grown for three weeks at two levels of CO2 (400 or 700 ppm) and two temperature regimes (26/21 or 31/26 °C, day/night). Plant dry mass, plant %N, protein concentrations, NO3− and NH4+ root uptake rates (using 15NO3 or 15NH4), and whole-plant N- and NO3--assimilation were measured. Plant growth, %N, protein concentration, and root N-uptake rate were each significantly affected only by CO2, while N- and NO3−-assimilation were significantly affected only by temperature. However, plants grown at eCO2 plus warming had the lowest concentrations of N and protein. These results suggest that one strategy breeding programs can implement to minimize the negative effects of eCO2 and warming on wheat tissue N would be to target the maintenance of root N uptake rate at eCO2 and N assimilation at higher growth temperatures.

Effect of N management approaches and planting densities on nitrogen accumulation by transplanted rice

Field experiments were conducted during 1998 and 1999 in June-September with rice variety ASD18 at the wetland farm, Tamil Nadu Agricultural University, Coimbatore, India to find out theeffect of N management approaches and planting densities on N accumulation by transplanted rice in a split plot design.The main plot consisted of three plant populations (33, 66 and 100 hills m-2) and the sub-plot treatments of five N management approaches. The results revealed thatthe average N uptake in roots and aboveground biomass progressively increased with growth stages. The mean root and aboveground biomass Nuptake were 26.1 to 130.6 and 6.4 to 17.8 kg ha-1, respectively. The N uptake of grain and straw was higher in theSesbania rostratagreen manuring + 150 kg N treatment, but it was not effective in increasing the grain yield. The mean total N uptake was found to be significantly lower at 33 hills m-2(76.9 kg ha-1) and increased with an increase in planting density (100.9 and 117.2 kg ha-1at 66 and 100 hills m-2density). N application had a significant influence on N uptake and the time course of N uptake in all the SPAD-guided N approaches. A significant regression coefficient was observed between the crop N uptake and grain yield. The relationship between cumulative N uptake at the flowering stage and the grain yield was quadratic at all three densities. The N uptake rate (µN) was maximum during the active tillering to panicle initiation period and declined sharply after that. In general, µNincreased with an increase in planting density and the increase was significant up to the panicle initiation to flowering period.thereafter, the N uptake rate was similar at densities of 66 and 100 hills m-2.

Growth analysis of dry matter accumulation and N uptake of forage maize cultivars affected by N supply

The productivity of eight forage maize cultivars (Zea mays L.) in response to N was investigated in a 3-year field experiment located in Northern Germany. Nitrogen fertilizer applications were zero, 50 and 150 kg N/ha given each year shortly after sowing. Each cultivar was grown on the same plot, beginning in 1993, following the ploughing up of a 2-year old grass sward, to which slurry had been added. Plants were sampled regularly for dry matter (DM) production and N uptake. A non-linear regression equation was used to compare the data. Growth analysis and N uptake characteristics (maximum crop growth rate, duration of maximum crop growth rate, period until maximum crop growth rate, maximum N uptake rate, duration of maximum N uptake rate, period until maximum N uptake rate) which derived from the function were used to compare the cultivars.The cultivars DM yield and N uptake were highest in 1993 and declined in the next two years partly due to a decrease in soil N mineralization following the ploughing of the grass sward and partly due to the drier weather conditions during the summers of 1994 and 1995. Duration of the maximum crop growth rate was greater during the cool year of 1993. In contrast, maximum crop growth rate was at its highest in the dry vegetation period of 1995. A significant N×cultivar interaction for growth analysis characteristics (P<0·05) was found in 1995. Cultivars with a high maximum crop growth rate (above the average value of the eight cultivars tested) and a short duration of maximum crop growth rate (below the average) accumulated more DM than those genotypes which showed an inverse relationship.Increasing N yield was determined by increased maximum N uptake rather than by a greater duration of maximum N uptake. A significant N×genotype interaction for N uptake parameters (P<0·05) was found in 1994 and 1995. With some exceptions, cultivars with a high maximum N uptake rate (above average) accumulated more N per unit area compared to those genotypes which had low uptake rates. The exceptions had a longer duration of uptake, which could not, however, compensate for the lower rate. Maximum N uptake rate occurred earlier and duration of maximum N uptake rate increased compared to the start and duration of maximum crop growth rate. Especially in 1995, the amount of N taken up before the day of maximum crop growth rate accounted for 71% of total N uptake. The N uptake rate and the amount of accumulated N until the day of maximum crop growth rate were highly correlated with DM yield.This result indicates the availability of genotypic variability in crop growth and N uptake rate to assist the improvement of DM yield by selection.