rsc complex
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2020 ◽  
Author(s):  
Richard W. Baker ◽  
Janice M. Reimer ◽  
Peter J. Carman ◽  
Tsutomu Arakawa ◽  
Roberto Dominguez ◽  
...  

AbstractChromatin remodelers regulate the position and composition of nucleosomes throughout the genome, producing different remodeling outcomes despite a shared underlying mechanism based on a conserved RecA DNA translocase. How this functional diversity is achieved remains unknown despite recent cryo-electron microscopy (cryo-EM) reconstructions of several remodelers, including the yeast RSC complex. To address this, we have focused on a RSC subcomplex comprising its ATPase (Sth1), the essential actin-related proteins (ARPs) Arp7 and Arp9, and the fungal-specific protein Rtt102. Combining cryo-EM and biochemistry of this subcomplex, which exhibits regulation of remodeling by the ARPs, we show that ARP binding induces a helical conformation in the HSA domain of Sth1, which bridges the ATPase domain with the bulk of the complex. Surprisingly, the ARP module is rotated by 120° in the subcomplex relative to full RSC about a pivot point previously identified as a regulatory hub in Sth1, suggesting that large conformational changes are part of Sth1 regulation and RSC assembly. We also show that an interaction between Sth1 and the nucleosome acidic patch, which appears to be conserved among SWI/SNF remodelers, enhances remodeling. Taken together, our structural data shed light on the assembly and function of the RSC complex.



Science ◽  
2019 ◽  
Vol 366 (6467) ◽  
pp. 833.3-833
Author(s):  
Steve Mao
Keyword(s):  


Science ◽  
2019 ◽  
Vol 366 (6467) ◽  
pp. 838-843 ◽  
Author(s):  
Youpi Ye ◽  
Hao Wu ◽  
Kangjing Chen ◽  
Cedric R. Clapier ◽  
Naveen Verma ◽  
...  

The RSC complex remodels chromatin structure and regulates gene transcription. We used cryo–electron microscopy to determine the structure of yeast RSC bound to the nucleosome. RSC is delineated into the adenosine triphosphatase motor, the actin-related protein module, and the substrate recruitment module (SRM). RSC binds the nucleosome mainly through the motor, with the auxiliary subunit Sfh1 engaging the H2A-H2B acidic patch to enable nucleosome ejection. SRM is organized into three substrate-binding lobes poised to bind their respective nucleosomal epitopes. The relative orientations of the SRM and the motor on the nucleosome explain the directionality of DNA translocation and promoter nucleosome repositioning by RSC. Our findings shed light on RSC assembly and functionality, and they provide a framework to understand the mammalian homologs BAF/PBAF and the Sfh1 ortholog INI1/BAF47, which are frequently mutated in cancers.



2018 ◽  
Vol 217 (7) ◽  
pp. 2445-2462 ◽  
Author(s):  
Tina L. Sing ◽  
Minnie P. Hung ◽  
Shinsuke Ohnuki ◽  
Godai Suzuki ◽  
Bryan-Joseph San Luis ◽  
...  

Ploidy is tightly regulated in eukaryotic cells and is critical for cell function and survival. Cells coordinate multiple pathways to ensure replicated DNA is segregated accurately to prevent abnormal changes in chromosome number. In this study, we characterize an unanticipated role for the Saccharomyces cerevisiae “remodels the structure of chromatin” (RSC) complex in ploidy maintenance. We show that deletion of any of six nonessential RSC genes causes a rapid transition from haploid to diploid DNA content because of nondisjunction events. Diploidization is accompanied by diagnostic changes in cell morphology and is stably maintained without further ploidy increases. We find that RSC promotes chromosome segregation by facilitating spindle pole body (SPB) duplication. More specifically, RSC plays a role in distributing two SPB insertion factors, Nbp1 and Ndc1, to the new SPB. Thus, we provide insight into a role for a SWI/SNF family complex in SPB duplication and ploidy maintenance.





2014 ◽  
Vol 56 (5) ◽  
pp. 653-666 ◽  
Author(s):  
Marla M. Spain ◽  
Suraiya A. Ansari ◽  
Rakesh Pathak ◽  
Michael J. Palumbo ◽  
Randall H. Morse ◽  
...  
Keyword(s):  
Pol Ii ◽  


Cell Reports ◽  
2013 ◽  
Vol 5 (6) ◽  
pp. 1714-1724 ◽  
Author(s):  
Rohith Srivas ◽  
Thomas Costelloe ◽  
Anne-Ruxandra Carvunis ◽  
Sovan Sarkar ◽  
Erik Malta ◽  
...  


PLoS ONE ◽  
2011 ◽  
Vol 6 (6) ◽  
pp. e21172 ◽  
Author(s):  
Edward J. Andress ◽  
Roman Holic ◽  
Mariola J. Edelmann ◽  
Benedikt M. Kessler ◽  
Veronica P. C. C. Yu
Keyword(s):  


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