Aspergillus diversity from the Gcwihaba Cave in Botswana and description of one new species

A fungal survey of the Gcwihaba Cave from Botswana found Aspergillus to be one of the more common fungal genera isolated. The 81 Aspergillus strains were identified using CaM sequences and comparing these to a curated reference dataset. Nineteen species were identified representing eight sections (sections Candidi, Circumdati, Flavi, Flavipedes, Nidulantes, Nigri, Terrei and Usti). One strain could not be identified. Morphological characterisation and multigene phylogenetic analyses confirmed it as a new species in section Flavipedes and we introduce it below as A. okavangoensis. The new species is most similar to A. iizukae, both producing conidiophores with vesicles typically wider than 20 µm. The new species, however, does not produce Hülle cells and its colonies grow slower than those of A. iizukae on CYA at 37 °C (14–15 vs 18–21 mm) and CREA (15–16 vs 23–41mm).

Pygmaeomycetaceae, a new root associated family in Mucoromycotina from the pygmy pine plains

ABSTRACTA new genus, Pygmaeomyces, and two new species are described based on phylogenetic analyses, phenotypic and ecological characters. The species delimitation was based on concordance of gene genealogies. The Pygmaeomyces cultures were isolated from the roots of mountain laurel (Kalmia latifolia) and pitch pine (Pinus rigida) from the acidic and oligotrophic New Jersey Pygmy Pine Plains; however, they likely have a broader distribution because their internal transcribed spacer (ITS) sequences have high similarity with a number of environmental sequences from multiple independent studies. Based on the phylogeny and phenotypical characters, a new family Pygmaeomycetaceae is proposed to accommodate this new lineage in Mucoromycotina. Pygmaeomycetaceae corresponds to Clade GS23, which was identified based on a sequence-only soil fungal survey and was believed to be a distinct new class. Compared to the culture-based methods, we observed that sequence-only analyses tend to over-estimate the taxonomic level. Results from this work will facilitate ecological and evolutionary studies on root-associated fungi.

Penicillium diversity in Canadian bat caves, including a new species, P. speluncae

Penicillium species were commonly isolated during a fungal survey of bat hibernacula in New Brunswick and Quebec, Canada. Strains were isolated from arthropods, bats, rodents (i.e. the deer mouse Peromyscus maniculatus), their dung, and cave walls. Hundreds of fungal strains were recovered, of which Penicillium represented a major component of the community. Penicillium strains were grouped by colony characters on Blakeslee's malt extract agar. DNA sequencing of the secondary identification marker, beta-tubulin, was done for representative strains from each group. In some cases, ITS and calmodulin were sequenced to confirm identifications. In total, 13 species were identified, while eight strains consistently resolved into a unique clade with P. discolor, P. echinulatum and P. solitum as its closest relatives. Penicillium speluncae is described using macroand micromorphological characters, multigene phylogenies (including ITS, beta-tubulin, calmodulin and RNA polymerase II second largest subunit) and extrolite profiles. Major extrolites produced by the new species include cyclopenins, viridicatins, chaetoglobosins, and a microheterogenous series of cyclic and linear tetrapeptides.

A First Assessment of the Conservation of the Mummified Human Remains in the Museo Egizio in Turin in the Framework of the “Mummy Conservation Project”

The Museo Egizio in Turin holds a unique collection of 116 mummified or skeletal human bodies or body parts. This collection is currently under study by the "Mummy Conservation Project", a collaborative venture of the Museo Egizio, the Institute for Mummy Studies of Eurac Research, the Soprintendenza Archeologia del Piemonte, and the Horus Group, whose aim is to improve mummy conservation techniques. In the context of this project, an assessment of the state of preservation of the mummified human remains was carried out by monitoring basic physical parameters (temperature, relative humidity and water activity) and by performing a fungal survey. The latter revealed the presence on the mummified material of fungal spores and mycelia that could possibly pose a biodegradative threat. However, all the current physical parameters show that the mummies are stored under optimal environmental conditions, which will suppress any microbial up-growth. ملخص البحث: يحتوي المتحف المصري في تورينو على مجموعة فريدة تضمّ 116 قطعة ما بين مومياوات بشرية محنطة أو هياكل عظمية أو أجزاء بشرية محنطة. حالياً هذه المجموعة قيد الدراسة من قبل "مشروع الحفاظ على المومياوات"، هو مشروع تعاوني ما بين المتحف المصري ومعهد دراسات المومياء لأبحاث يوراك ومكتب الحفاظ على التراث الأثري في مقاطعة بييمونتي ومجموعة حورس، التي تهدف إلى تحسين تقنيات الحفاظ على المومياوات. في نطاق هذا المشروع، تم تقييم حالة الحفظ للبقايا البشرية المحنطة من خلال مراقبة العوامل الفيزيائية الأساسية (درجة الحرارة والرطوبة النسبية ونشاط الجزيئات المائية) وإجراء مسح للفطور. كشفت هذه الأخيرة عن وجود فطور وبكتريات فطرية على المواد المحنطة والتي يمكن أن تشكل تهديداً بالتحلل الحيوي. ومع ذلك تظهر جميع المقاييس الفيزيائية الحالية أن المومياوات يتم تخزينها في ظل ظروف بيئية مثالية، والتي سوف تثبط أي نمو للميكروبات.

Polypores from a Brazilian pine forest in Southern Brazil: pileate species

A fungal survey in the National Forest of São Francisco de Paula, in southern Brazil, displayed 38 pileate polypores species (eight Hymenochaetales and 30 Polyporales). Amauroderma coltricioides T.W. Henkel, Aime & Ryvarden and Inonotus fulvomelleus Murrill are recorded for the fist time from Brazil, whereas Antrodiella multipileata Log.-Leite & J.E. Wright and Junghuhnia minuta I. Lindblad & Ryvarden are new records to Rio Grande do Sul State. Keys to species and remarks on the taxa are presented.