recombinant expression vector
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Immunization with a Recombinant Expression Vector Encoding NS3/NS4A of Hepatitis C Virus Genotype 3a Elicits Cell-Mediated Immune Responses in C57BL/6 Mice

2016 ◽  
Vol 29 (3) ◽  
pp. 138-147 ◽  
Author(s):  
Mohammad Amin Behzadi ◽  
Abdolvahab Alborzi ◽  
Mehdi Kalani ◽  
Gholamreza Pouladfar ◽  
Mehdi Dianatpour ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Immune Responses ◽  
Expression Vector ◽  
Recombinant Expression ◽  
Virus Genotype ◽  
Recombinant Expression Vector ◽  
Genotype 3A

Expression and Purification of His-chIFN-α and its Antiviral Activity Assay

2013 ◽  
Vol 750-752 ◽  
pp. 1515-1519
Author(s):  
Yue Ma ◽  
Min Hui Long ◽  
Ai Po Diao
Keyword(s):  
Antiviral Activity ◽  
Expression Vector ◽  
Recombinant Expression ◽  
Metal Chelate ◽  
Activity Assay ◽  
Expression And Purification ◽  
E Coli ◽  
Sds Page ◽  
Recombinant Expression Vector ◽  
Metal Chelate Affinity Chromatography

In this paper, Chicken alpha interferon (IFN-α) gene was cloned into pUC19-His expression vector, then the recombinant expression vector was transformed into host bacteria E. coli BL21. The recombinant chicken IFN-α was induced to express by IPTG, then the protein expression was analyzed with SDS-PAGE. Under the condition that the recombinant protein was induced to express with 1 mM IPTG at 37 °C, the expressed protein was inclusion body. His-chIFN-α was purified by Ni-metal chelate affinity chromatography. His-chIFN-α was shown to inhibit the replication of Newcastle disease virus in CEF cells.

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