scholarly journals Generation of pcdna 3.1+-gh as a recombinant expression vector of ostrich growth hormone cdna in saccharomyces cerevisiae

2015 ◽  
pp. 99-104
Author(s):  
H. Fathpour ◽  
A. Doosti ◽  
P. Ghasemi-Dehkordi ◽  
G. Shirazi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Hormone ◽  
Expression Vector ◽  
Recombinant Expression ◽  
Recombinant Expression Vector

scholarly journals Constitutive Optimized Production of Streptokinase inSaccharomyces cerevisiaeUtilizing Glyceraldehyde 3-Phosphate Dehydrogenase Promoter ofPichia pastoris

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Ravi N. Vellanki ◽  
Ravichandra Potumarthi ◽  
Kiran K. Doddapaneni ◽  
Naveen Anubrolu ◽  
Lakshmi N. Mangamoori
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pichia Pastoris ◽  
Expression Vector ◽  
Recombinant Expression ◽  
Expression System ◽  
Nitrogen Sources ◽  
Clot Lysis ◽  
Process Conditions ◽  
Scale Production ◽  
Activation Assay

A novel expression vector constructed from genes ofPichia pastoriswas applied for heterologous gene expression inSaccharomyces cerevisiae. Recombinant streptokinase (SK) was synthesized by cloning the region encoding mature SK under the control of glyceraldehyde 3-phosphate dehydrogenase (GAP) promoter ofPichia pastorisinSaccharomyces cerevisiae. SK was intracellularly expressed constitutively, as evidenced by lyticase-nitroanilide and caseinolytic assays. The functional activity was confirmed by plasminogen activation assay andin vitroclot lysis assay. Stability and absence of toxicity to the host with the recombinant expression vector as evidenced by southern analysis and growth profile indicate the application of this expression system for large-scale production of SK. Two-stage statistical approach, Plackett-Burman (PB) design and response surface methodology (RSM) was used for SK production medium optimization. In the first stage, carbon and organic nitrogen sources were qualitatively screened by PB design and in the second stage there was quantitative optimization of four process variables, yeast extract, dextrose, pH, and temperature, by RSM. PB design resulted in dextrose and peptone as best carbon and nitrogen sources for SK production. RSM method, proved as an efficient technique for optimizing process conditions which resulted in 110% increase in SK production, 2352 IU/mL, than for unoptimized conditions.

Immunization with a Recombinant Expression Vector Encoding NS3/NS4A of Hepatitis C Virus Genotype 3a Elicits Cell-Mediated Immune Responses in C57BL/6 Mice

2016 ◽  
Vol 29 (3) ◽  
pp. 138-147 ◽  
Author(s):  
Mohammad Amin Behzadi ◽  
Abdolvahab Alborzi ◽  
Mehdi Kalani ◽  
Gholamreza Pouladfar ◽  
Mehdi Dianatpour ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Immune Responses ◽  
Expression Vector ◽  
Recombinant Expression ◽  
Virus Genotype ◽  
Recombinant Expression Vector ◽  
Genotype 3A

Expression and Purification of His-chIFN-α and its Antiviral Activity Assay

2013 ◽  
Vol 750-752 ◽  
pp. 1515-1519
Author(s):  
Yue Ma ◽  
Min Hui Long ◽  
Ai Po Diao
Keyword(s):  
Antiviral Activity ◽  
Expression Vector ◽  
Recombinant Expression ◽  
Metal Chelate ◽  
Activity Assay ◽  
Expression And Purification ◽  
E Coli ◽  
Sds Page ◽  
Recombinant Expression Vector ◽  
Metal Chelate Affinity Chromatography

In this paper, Chicken alpha interferon (IFN-α) gene was cloned into pUC19-His expression vector, then the recombinant expression vector was transformed into host bacteria E. coli BL21. The recombinant chicken IFN-α was induced to express by IPTG, then the protein expression was analyzed with SDS-PAGE. Under the condition that the recombinant protein was induced to express with 1 mM IPTG at 37 °C, the expressed protein was inclusion body. His-chIFN-α was purified by Ni-metal chelate affinity chromatography. His-chIFN-α was shown to inhibit the replication of Newcastle disease virus in CEF cells.

scholarly journals Construction of a universal recombinant expression vector that regulates the expression of human lysozyme in milk

2018 ◽  
Vol 5 (3) ◽  
pp. 382
Author(s):  
Shen LIU ◽  
Shengzhe SHANG ◽  
Xuezhen YANG ◽  
Huihua ZHANG ◽  
Dan LU ◽  
...  
Keyword(s):  
Expression Vector ◽  
Recombinant Expression ◽  
Human Lysozyme ◽  
Recombinant Expression Vector
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