Expression and Purification of His-chIFN-α and its Antiviral Activity Assay
2013 ◽
Vol 750-752
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pp. 1515-1519
In this paper, Chicken alpha interferon (IFN-α) gene was cloned into pUC19-His expression vector, then the recombinant expression vector was transformed into host bacteria E. coli BL21. The recombinant chicken IFN-α was induced to express by IPTG, then the protein expression was analyzed with SDS-PAGE. Under the condition that the recombinant protein was induced to express with 1 mM IPTG at 37 °C, the expressed protein was inclusion body. His-chIFN-α was purified by Ni-metal chelate affinity chromatography. His-chIFN-α was shown to inhibit the replication of Newcastle disease virus in CEF cells.
2015 ◽
Vol 16
(1)
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pp. 31
2013 ◽
Vol 310
◽
pp. 157-161
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2009 ◽
Vol 29
(1)
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pp. 45-49
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2015 ◽
Vol 16
(1)
◽
pp. 31
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2008 ◽
Vol 2008
◽
pp. 1-8
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2004 ◽
Vol 36
(4)
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pp. 309-313
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