IDENTIFICATION OF NON-TUBERCULOSIS MYCOBACTERIA ISOLATED FROM ENVIRONMENTAL OBJECTS IN THE REPUBLIC OF TATARSTAN

The purpose of this work was to identify mycobacteria of non-tuberculosis type isolated from environmental objects in the Republic of Tatarstan. The article presents the result of identification of mycobacteria of non-tuberculous type in samples of washout from environmental objects obtained from farms with an identified species list of circulating mycobacteria of non-tuberculous type from reacting to tuberculin cattle by polymerase chain reaction in real time. In the course of our research, we found that nonspecific allergic reactions to bovine tuberculin in these farms are caused by contamination with mycobacteria of a non-tuberculosis type of livestock premises and soils near farms.

Prevalence of Bovine Tuberculosis among Buffaloes Innorth Karnataka

The present study was undertaken to assess the prevalence of bovine tuberculosis among buffaloes in Belgaum, Dharwad and Bagalkote districts of North Karnataka, for a period of eight years (2002 to 2009). A total of 2184 buffaloes belonging to 30 organized farms were screened by single intradermal tuberculin test using bovine tuberculin Purified protein derivative. Overall prevalence of tuberculosis among buffaloes was found to be 0.96 per cent. Prevalence was higher in females (0.97%) than males (0.84%) and in Murrah (1.02 %) than Surthi (0.85 %). Analysis of the temporal pattern revealed a gradual declining trend over a period of eight years.

Mycobacterial infections in cattle in the CzechRepublic during 1990–1999

In the nineties, cattle stocks gradually decreased from 3 506 222 head of cattle in 1990 to 1 657 337 head in 1999. Skin testing of cattle was carried out annually using bovine tuberculin. Animals for sale were also simultaneously tested with avian tuberculin. In records from 1991 to 1999 a total of 14 611 393 bovine tuberculin skin tests and 611 405 simultaneous avian tuberculin skin tests are registered. A total of 1 457 (0.01%) animals reacted positively with bovine tuberculin and 1 790 (0.29%) with avian tuberculin. In the period monitored a total of 7 268 274 head of cattle were slaughtered and given veterinary hygienic examinations. Statistical data on the post-mortem detection of tuberculous lesions have been available for nine years since 1992 when tuberculous lesions were found in 1 186 (0.019%) out of 6 273 441 slaughtered animals. Mycobacteria were isolated from the organs of only 561 (17.5%) out of 3 202 culturally examined animals. M. bovis only was isolated from 48 (8.6%) animals originating from seven herds (two infected herds in 1991, 1992 and 1994 and one infected herd in 1995): four outbreaks were detected by annual skin testing, one outbreak by movement tuberculin skin testing and two outbreaks by the detection of tuberculous lesions at slaughter. M. avium complex strains of serotypes 1, 2 and 3 and of genotypes IS901+ and IS1245+ were isolated from 331 (59.0%) animals and strains of serotypes 4 to 6, 8 to 11 and 21 and of genotypes IS901- and IS1245+ were isolated from 132 (23.5%) animals. Potentially pathogenic bacteria of the M. chelonae, M. terrae, M. phlei and M. fortuitum species were isolated from 50 (8.9%) animals. Neither miliary nor generalised tuberculosis was found in any of the animals. Between 1996 and 1999, the proportion of cattle in which tuberculous lesions were recorded decreased.